2007
DOI: 10.1074/jbc.m704555200
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Crystal Structure of Tapes japonica Lysozyme with Substrate Analogue

Abstract: Tapes japonica lysozyme (TJL) is classified as a member of the recently established i-type lysozyme family. In this study, we solved the crystal structure of TJL complexed with a trimer of N-acetylglucosamine to 1.6 Å resolution. Based on structure and mutation analyses, we demonstrated that Glu-18 and Asp-30 are the catalytic residues of TJL. Furthermore, the present findings suggest that the catalytic mechanism of TJL is a retaining mechanism that proceeds through a covalent sugar-enzyme intermediate. On the… Show more

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Cited by 61 publications
(44 citation statements)
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References 47 publications
(74 reference statements)
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“…In the cDNA sequence analysis of VPL, two extra amino acids, F-S, have been shown to be present at the C-terminus. 16) This extra sequence did not occur in the expressed protein 15) and a similar sequence has been reported for VPL cDNA. 46) Goto et al 16) have reported the crystal structure of the VPL-(GlcNAc)3 complex.…”
Section: Determination Of the Complete Amino Acid Sequence Of Mll-amentioning
confidence: 94%
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“…In the cDNA sequence analysis of VPL, two extra amino acids, F-S, have been shown to be present at the C-terminus. 16) This extra sequence did not occur in the expressed protein 15) and a similar sequence has been reported for VPL cDNA. 46) Goto et al 16) have reported the crystal structure of the VPL-(GlcNAc)3 complex.…”
Section: Determination Of the Complete Amino Acid Sequence Of Mll-amentioning
confidence: 94%
“…The lytic activity of bivalve lysozymes is sensitive to the salt concentrations of the assay solution. 16,19) Similarly, with increasing salt concentrations, oyster and blue mussel lysozyme activities against Micrococcus luteus increased at salt concentrations of up to 0.1 M salt. At higher salt concentrations, the lytic activity decreased.…”
Section: The Complete Amino Acid Sequence and Enzymatic Properties Ofmentioning
confidence: 99%
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“…Склонность к образованию димерных комплексов показана и для высокогомологичного Дест-Лиз лизоцима из морского моллюска Tapes japonica [9]. Более того, авторы показали, что в присутствии 0,15 М NaCl, то есть в условиях, вызывающих диссоциацию димера, наблюдается резкое увеличение активности фермента, что указывает на важную функцию солевого компонента.…”
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