Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa

Rasheed, Masooma; Garnett, James A.; Pérez‐Dorado, Inmaculada; Muhl, Daniela; Filloux, Alain; Matthews, Stephen

doi:10.1016/j.bbapap.2016.07.010

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…Pseudomonas aeruginosa is the most frequent gram‐negative bacterium associated with nosocomial and life‐threatening chronic infections (Rasheed et al., ). Oxygen is vital for such organism due to its absolutely aerobic nature.…”

Section: Resultsmentioning

confidence: 99%

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The antibacterial effect of whey protein–alginate coating incorporated with the lactoperoxidase system on chicken thigh meat

Molayi

Ehsani

Yousefi

2018

Food Science & Nutrition

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Nowadays, the environmental problems due to the use of synthetic films and packages have caused the production of natural edible coatings or films. The aim of this study was to produce an edible whey protein–alginate coating with different concentrations of lactoperoxidase system to control the microbial load and increase the shelf life of chicken thigh meat stored in refrigerated condition (4 ± 1°C). So, after the provision of the alginate–whey protein coating incorporated with the lactoperoxidase system (at concentrations of 2%, 4%, and 6% in alginate–whey protein solution), microbial experiments were conducted for the period of 8 days. Three batches of organisms, including total aerobic mesophilic bacteria, Enterobacteriaceae, and Pseudomonas aeruginosa in samples, were tested by culturing in appropriate conditions. Results indicated that the coating had a substantial inhibitory effect on all lots. Also, the antimicrobial activity of coating increased with increase in lactoperoxidase system concentration in alginate–whey protein coating.

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Section: Resultsmentioning

confidence: 99%

“…So, it can prevent the growth of absolute aerobic bacteria depending on its concentration in coatings or films. Regarding the relationship between P. aeruginosa and edible films, it should be mentioned that P. aeruginosa is often used as a model organism for the study of biofilms (Rasheed et al., ).…”

Section: Resultsmentioning

confidence: 99%

The antibacterial effect of whey protein–alginate coating incorporated with the lactoperoxidase system on chicken thigh meat

Molayi

Ehsani

Yousefi

2018

Food Science & Nutrition

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“…Phyre2, a sequence/structure comparison tool [ 34 ], revealed that there are distinct adhesins present in the UshC and YraJ subgroups. While some are more similar to the LpfD adhesin [ 35 ], others are more similar to the Pseudomonas adhesin CupB6 ([ 36 ]; figure 4 b ; electronic supplementary material, table S5). This dichotomy is further reflected in the chaperones that assemble the adhesin subunits (electronic supplementary material, figure S2), which cluster similarly to the phylogenetic history of the usher proteins, largely consistent with an LGT event acting upon the entire chaperone–usher operon, although the ordering of the inner branches is not stable with low support values.…”

Section: Resultsmentioning

confidence: 99%

Constraints on lateral gene transfer in promoting fimbrial usher protein diversity and function

et al. 2017

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Fimbriae are long, adhesive structures widespread throughout members of the family Enterobacteriaceae. They are multimeric extrusions, which are moved out of the bacterial cell through an integral outer membrane protein called usher. The complex folding mechanics of the usher protein were recently revealed to be catalysed by the membrane-embedded translocation and assembly module (TAM). Here, we examine the diversity of usher proteins across a wide range of extraintestinal (ExPEC) and enteropathogenic (EPEC) Escherichia coli, and further focus on a so far undescribed chaperone–usher system, with this usher referred to as UshC. The fimbrial system containing UshC is distributed across a discrete set of EPEC types, including model strains like E2348/67, as well as ExPEC ST131, currently the most prominent multi-drug-resistant uropathogenic E. coli strain worldwide. Deletion of the TAM from a naive strain of E. coli results in a drastic time delay in folding of UshC, which can be observed for a protein from EPEC as well as for two introduced proteins from related organisms, Yersinia and Enterobacter. We suggest that this models why the TAM machinery is essential for efficient folding of proteins acquired via lateral gene transfer.

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“…Part of the chaperone-usher system in P. aeruginosa , known to assemble pili on the bacterial surface, the fimbrial subunit CupB6 (Target 37) of P. aeruginosa is the tip adhesion subunit that is attached to the main pilin shaft. The surface-exposed polyproline helix of CupB6 is part of the adhesion domain, and is believed to mediate a range of protein-protein interactions (Rasheed et al, 2016). In S. aureus , Target ID 37 or ‘biofilm-associated surface protein’, mediates intercellular adhesion and biofilm formation, with the N-terminal lobe of the protein identified to have a significant role (Ma et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

‘Targeting’ the Search: An Upgraded Structural and Functional Repository of Antimicrobial Peptides for Biofilm Studies (B-AMP v2.0) with a Focus on Biofilm Protein Targets

Ravichandran

Avatapalli

Narasimhan

et al. 2022

Preprint

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Bacterial biofilms, often as multispecies communities, are recalcitrant to conventional antibiotics, making the treatment of biofilm infections a challenge. There is a push towards developing novel anti-biofilm approaches, such as antimicrobial peptides (AMPs), with activity against specific biofilm targets. In previous work, we developed Biofilm-AMP, a structural and functional repository of AMPs for biofilm studies (B-AMP v1.0) with more than 5000 structural models of AMPs and a vast library of AMP annotations to existing biofilm literature. In this study, we present an upgraded version of B-AMP, with a focus on existing and novel bacterial biofilm targets. B-AMP v2.0 hosts a curated collection of 2502 biofilm protein targets across 473 bacterial species, with structural protein models and functional annotations from PDB, UniProt, and PubMed databases. The biofilm targets can be searched for using the name of the source organism, and function and type of protein, and results include designated Target IDs (unique to B-AMP v2.0), UniProt IDs, 3D predicted protein structures, PDBQT files, pre-defined protein function, and relevant scientific literature. To present an example of the combined applicability of both, the AMP and biofilm target libraries in the repository, we present two case studies. In the first case study, we expand an in silico pipeline to evaluate AMPs against a single biofilm target in the multidrug resistant, bacterial pathogen Corynebacterium striatum, using 3D protein-peptide docking models from previous work and Molecular Dynamics simulations (~1.18 microseconds). In the second case study, we build an in silico pipeline to identify candidate AMPs (using AMPs with both anti-Gram positive and anti-Gram negative activity) against two biofilm targets with a common functional classification in Pseudomonas aeruginosa and Staphylococcus aureus, widely-encountered bacterial co-pathogens. With its enhanced structural and functional capabilities, B-AMP v2.0 serves as a comprehensive resource for AMP investigations related to biofilm studies. B-AMP v2.0 is freely available at https://b-amp.karishmakaushiklab.com and will be regularly updated with structural models of AMPs and biofilm targets, as well as 3D protein-peptide interaction models for key biofilm-forming pathogens.

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Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa

Cited by 5 publications

References 34 publications

The antibacterial effect of whey protein–alginate coating incorporated with the lactoperoxidase system on chicken thigh meat

The antibacterial effect of whey protein–alginate coating incorporated with the lactoperoxidase system on chicken thigh meat

Constraints on lateral gene transfer in promoting fimbrial usher protein diversity and function

‘Targeting’ the Search: An Upgraded Structural and Functional Repository of Antimicrobial Peptides for Biofilm Studies (B-AMP v2.0) with a Focus on Biofilm Protein Targets

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