2023
DOI: 10.1002/prot.26498
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Crystal structure of the dimerized of porcine circovirus type II replication‐related protein Rep′

Abstract: Porcine circovirus type 2 (PCV2) can cause porcine circovirus-associated disease (PCVAD), which causes significant economic losses to the global pig industry annually. There are no effective antiviral drugs used to control and treat PCV2, and prevention is mainly obtained through vaccination. PCV2 genome replicates through the rolling circle replication (RCR) mechanism involving Rep and Rep 0 , so analyzing the holistic structure of Rep and Rep 0 will help us better understand the replication process of PCV2. … Show more

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Cited by 4 publications
(2 citation statements)
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“…Among them, the endonuclease domain exhibits endonuclease activity, nicking and ligating the viral origin of the replication sequence; the oligomeric domain facilitates the formation of viral multimeric complexes; the ATPase domain has ATPase-dependent helicase activity, which unwinds double-stranded genomes by hydrolysing ATP [12]. In contrast to Rep, Rep' has only nucleic acid endonuclease activity and can form a homodimer through its C-terminal domain [13]. When Rep unwinds double-stranded genomes, it functions as a helicase and the protein assembles into hexamers, which fulfil the characteristics of the superfamily 3 (SF3) helicase [12,14].…”
Section: Introductionmentioning
confidence: 99%
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“…Among them, the endonuclease domain exhibits endonuclease activity, nicking and ligating the viral origin of the replication sequence; the oligomeric domain facilitates the formation of viral multimeric complexes; the ATPase domain has ATPase-dependent helicase activity, which unwinds double-stranded genomes by hydrolysing ATP [12]. In contrast to Rep, Rep' has only nucleic acid endonuclease activity and can form a homodimer through its C-terminal domain [13]. When Rep unwinds double-stranded genomes, it functions as a helicase and the protein assembles into hexamers, which fulfil the characteristics of the superfamily 3 (SF3) helicase [12,14].…”
Section: Introductionmentioning
confidence: 99%
“…Other parts of the Rep structure have only analysed using cryo-electron microscopy at a lower resolution [12]. Furthermore, the X-ray crystallography method has resolved the structures of the entire Rep' protein [13]. Currently, the complete structure of PCV2 Rep has yet to be fully elucidated, and there are still many aspects of the PCV2 replication mechanism that still need to be clarified.…”
Section: Introductionmentioning
confidence: 99%