2011
DOI: 10.1016/j.str.2011.02.001
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Crystal Structure of Type III Glutamine Synthetase: Surprising Reversal of the Inter-Ring Interface

Abstract: Glutamine synthetases are ubiquitous, homo-oligomeric enzymes essential for nitrogen metabolism. Unlike types I and II, which are well described both structurally and functionally, the larger, type IIIs are poorly characterized despite their widespread occurrence. An understanding of the structural basis for this divergence and the implications for design of type-specific inhibitors has, therefore, been impossible. The first crystal structure of a GSIII enzyme, presented here, reveals a conservation of the GS … Show more

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Cited by 51 publications
(70 citation statements)
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“…S2 in the supplemental material). In addition, GSIII-1 and GSIII-2 displayed strong conservation of all five GS conserved regions and four GS type III-specific conserved regions (11,44). Furthermore, key conserved amino acid residues essential for function of the GSIII protein in signature motifs I to IV and motif C in R. albus 8 were all highly conserved within the P. ruminicola 23 GSIII amino acid sequence (2).…”
Section: Discussionmentioning
confidence: 98%
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“…S2 in the supplemental material). In addition, GSIII-1 and GSIII-2 displayed strong conservation of all five GS conserved regions and four GS type III-specific conserved regions (11,44). Furthermore, key conserved amino acid residues essential for function of the GSIII protein in signature motifs I to IV and motif C in R. albus 8 were all highly conserved within the P. ruminicola 23 GSIII amino acid sequence (2).…”
Section: Discussionmentioning
confidence: 98%
“…GSIII was first described in Bacteroides fragilis (25) and also assembles as a homododecamer, comprising larger subunits (M r , ca. 75,000) than those of GSI or GSII (2,40,44). GSIII have been identified in Butyrivibrio fibrisolvens, Ruminococcus albus 8, and some cyanobacteria (2,11,22,35).…”
mentioning
confidence: 99%
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“…Each B. subtilis GS subunit contains 19 b strands and 15 a helices. All GS structures solved to date are dodecamers or decamers that are comprised of two stacked rings (either hexamers or pentamers) (Liaw and Eisenberg 1994;Eisenberg et al 2000;Unno et al 2006;van Rooyen et al 2011). However, because GS active sites are formed at the interfaces between two subunits within a single ring, hexamer, or pentamer, it has been unclear what role the double-ring structure may play in GS function.…”
Section: Regulation Of Glnr Function By Its Nitrogen Sensor Domainmentioning
confidence: 99%