Crystallinity and compositional changes in carbonated apatites: Evidence from 31P solid-state NMR, Raman, and AFM analysis

McElderry, John David P.; Zhu, Peizhi; Mroué, Kamal H.; Xu, Jiadi; Pavan, Barbara; Fang, Ming; Zhao, Guisheng; McNerny, Erin M.B.; Kohn, David H.; Franceschi, Renny T.; Holl, Mark M. Banaszak; Tecklenburg, Mary M; Ramamoorthy, Ayyalusamy; Morris, Michael D.

doi:10.1016/j.jssc.2013.08.011

Cited by 83 publications

(76 citation statements)

References 58 publications

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“…Although the specific mechanisms responsible are not clear, changes in crystallinity because of BAPN treatment is an interesting finding that warrants further investigation using more direct methods of evaluating changes to the crystalline structure. 27 Another study demonstrated no effects on crystallinity/maturity with BAPN treatment, 13 but these conflicting data may be due to differences in dose (B780 vs 300 mg kg À 1 ), method of delivery (dietary vs subcutaneous injection), animal model (rat vs mouse) and anatomical site (vertebral crosssection vs native surface of femur).…”

Section: Discussionmentioning

confidence: 99%

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Hammond¹,

Wallace²

2015

BoneKEy Reports

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This study evaluated the effects of reduced enzymatic crosslinking, exercise and the ability of exercise to prevent the deleterious impact of reduced crosslinking on collagen D-spacing. Eight-week-old female mice were divided into four weight-matched groups receiving daily injections of either phosphate-buffered saline (PBS) or 300 mg kg À 1 b-aminopropionitrile (BAPN) while undergoing normal cage activity (Sed) or 30 min per day of treadmill exercise (Ex) for 21 consecutive days. BAPN caused a downward shift in the D-spacing distribution in Sed BAPN compared with Sed PBS (Po0.001) but not in Ex BAPN (P ¼ 0.429), indicating that exercise can prevent changes in collagen morphology caused by BAPN. Exercise had no effect on D-spacing in PBS control mice (P ¼ 0.726), which suggests that exercise-induced increases in lysyl oxidase may be a possible mechanism for preventing BAPN-induced changes in D-spacing. The D-spacing changes were accompanied by an increase in mineral crystallinity/maturity due to the main effect of BAPN (P ¼ 0.016). However, no changes in nanoindentation, reference point indentation or other Raman spectroscopy parameters were observed. The ability of exercise to rescue BAPN-driven changes in collagen morphology necessitates further research into the use of mechanical stimulation as a preventative therapy for collagen-based diseases.

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Section: Discussionmentioning

confidence: 99%

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Hammond¹,

Wallace²

2015

BoneKEy Reports

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“…Positional disorder affects the length, and thus the strength and vibrational frequency, of C-O bonds (Bischoff et al, 1985). An increase in disorder leads to an increase in the distribution of C-O bond lengths, causing an increase in ν 1 peak width (Bischoff et al, 1985;Addadi et al, 2003;Lin et al, 2007;Wang et al, 2012;McElderry et al, 2013;Perrin et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy

DeCarlo¹,

D’Olivo²,

Foster³

et al. 2017

Biogeosciences

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Abstract. Quantifying the saturation state of aragonite ( Ar ) within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enable the determination of calcifying fluid pH and [CO ]/K sp ) has proved elusive. Here we test a new technique for deriving Ar based on Raman spectroscopy. First, we analysed abiogenic aragonite crystals precipitated under a range of Ar from 10 to 34, and we found a strong dependence of Raman peak width on Ar with no significant effects of other factors including pH, Mg/Ca partitioning, and temperature. Validation of our Raman technique for corals is difficult because there are presently no direct measurements of calcifying fluid Ar available for comparison. However, Raman analysis of the international coral standard JCp-1 produced Ar of 12.3 ± 0.3, which we demonstrate is consistent with published skeletal Mg/Ca, Sr/Ca, B/Ca, δ 11 B, and δ 44 Ca data. Raman measurements are rapid (≤ 1 s), high-resolution (≤ 1 µm), precise (derived Ar ± 1 to 2 per spectrum depending on instrument configuration), accurate ( ±2 if Ar < 20), and require minimal sample preparation, making the technique well suited for testing the sensitivity of coral calcifying fluid Ar to ocean acidification and warming using samples from natural and laboratory settings. To demonstrate this, we also show a high-resolution time series of Ar over multiple years of growth in a Porites skeleton from the Great Barrier Reef, and we evaluate the response of Ar in juvenile Acropora cultured under elevated CO 2 and temperature.

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“…Hydroxyapatite (HA) is the main inorganic component of human bone mineral, and the content of carbonate in human bone mineral is about 5-8 wt % [1, 2]. Carbonate ions in carbonated hydroxyapatites (CHA) substitute both the phosphate and hydroxyl sites of the HA structure and each is called A-type CHA and B-type CHA, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…The characteristic peaks at 428 and 588 cm−1 were assigned to υ2 and υ4 mode, respectively [28]. As the carbonate content increases, the strongest symmetric stretch υ1 mode of PO4 3− at 960 cm−1 becomes broader, indicating the decrease of crystallinity of apatite lattices [1,29]. The peak at 1070 cm−1 can be assigned to the B-type υ1 CO3 2− mode [30, 31].…”

mentioning

confidence: 99%

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

Xue¹,

Chen²,

Huang³

et al. 2016

Nano Online

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(http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Highly crystalline carbonated hydroxyapatite (CHA) nanorods with different carbonate contents were synthesized by a novel hydrothermal method. The crystallinity and chemical structure of synthesized nanorods were studied by Fourier transform infrared spectroscopy (FTIR), X-ray photo-electronic spectroscopy (XPS), X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscopy (TEM). The biocompatibility of synthesized CHA nanorods was evaluated by cell viability and alkaline phosphatase (ALP) activity of MG-63 cell line. The biocompatibility evaluation results show that these CHA nanorods are biologically active apatites and potentially promising bone-substitute biomaterials for orthopedic application.Keywords: Hydroxyapatite; Carbonated hydroxyapatite; Nanorods; Cell viability; Alkaline phosphatase activity; MG-63 cell BackgroundHydroxyapatite (HA) is the main inorganic component of human bone mineral, and the content of carbonate in human bone mineral is about 5-8 wt % [1, 2]. Carbonate ions in carbonated hydroxyapatites (CHA) substitute both the phosphate and hydroxyl sites of the HA structure and each is called A-type CHA and B-type CHA, respectively. Predominantly, the carbonate ions are present as B-type carbonates in natural bone minerals [3]. Synthetic CHAs have been widely used in a variety of biomedical applications including osteoconductive coatings [4][5][6], bone-substitute biomaterials [7], and vehicles for drug delivery [ 8]. Recently, hydoxyapatite nanorods have been prepared by an ethanol-induced method [9], liquid crystals [10], sonochemical synthesis [11], sol-gel method [12], and hydrothermal reaction [13,14]. However, few methods have been reported for the preparation of carbonated hydroxyapatite nanorods with different carbonate contents. Since carbonate ion substitution in the apaptite lattice plays a major role in the biochemistry and physical properties of biological apatites, it is important to develop convenient ways for the synthesis of CHA nanorods with different carbonate contents and understand how various carbonate contents affect the crystal structure and biocompatibility of CHA nanorods. Table 1 Synthesizing materials for preparing HA and CHA nanorods SamplesCaThe hydrothermal method is a typical process which has been widely used in synthesis of inorganic materials for its good repeatability and crystallinity control [15][16][17]. In this study, we developed a hydrothermal process to synthesize carbonated hydroxyapatite nanorods with different carbonate contents, using ethylene diamine tetraacetic aci...

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Crystallinity and compositional changes in carbonated apatites: Evidence from 31P solid-state NMR, Raman, and AFM analysis

Cited by 83 publications

References 58 publications

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Exercise prevents β-aminopropionitrile-induced morphological changes to type I collagen in murine bone

Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy

Hydrothermal Synthesis and Biocompatibility Study of Highly Crystalline Carbonated Hydroxyapatite Nanorods

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