Crystallisation of calcium oxalate dihydrate in normal urine in presence of sodium copper chlorophyllin

Tawashi, R.; Cousineau, Marie‐Marthe; Denis, Gustave

doi:10.1007/bf00255940

Cited by 11 publications

(5 citation statements)

References 8 publications

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“…For microscopy, 1 ml of the urine sample was centrifuged at 3,000 rpm for 10 min, and then 950 μl of the supernatant was discarded, and 10 μl of the vortex-mixed sediment was then transferred to a hemocytometer. The type and number of the crystals were identified and counted per high power field (HPF) using an Olympus light microscope (Tawashi et al, 1982).…”

Section: Urine Collection and Analysismentioning

confidence: 99%

Effect of curcumin in the prevention of experimentally induced nephrolithiasis in rats by ethylene glycol and Vitamin D3

Gandhi¹,

Balaraman²

2009

Oriental Pharmacy and Experimental Medicine

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Curcumin (CMN) is known to have beneficial role in anorexia, coryza, cough, diabetic wounds, and hepatic disorders apart from its inherent antioxidant effects. Therefore, the present study was aimed to evaluate antioxidant effect of CMN in prevention of nephrolithiasis in rats-induced by ethylene glycol (EG) and Vitamin D3 (Vit. D3). Male Wistar rats (175-200 g) were randomized in groups like control, EG + Vit. D3 induced nephrolithiatiatic rats, CMN treated rats, CMN + EG + Vit. D3 treated rats, Vit. E + EG + Vit. D3 treated rats. Urine was collected weekly throughout the experimental protocol and estimated for calcium oxalate (CaO) count. After completion of experimental protocol serum was estimated for blood urea nitrogen and creatinine. Both the kidneys were excised and used to evaluate levels of biomarkers of oxidative stress and calcium oxalate crystal deposition by histopathological studies. Administration of EG and Vit. D3 to rats resulted in increased oxidative stress, hyperoxaluria and renal deposition of CaO crystals. Supplementation with CMN improves kidney function, reduces elevated oxidative stress, urinary oxalate level and renal deposition of CaO which shows its protective action in nephrolithiasis. The increased deposition of stone in the kidney and stone forming constituents of nephrolithiatic rats were effectively lowered by treatment of CMN.

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Section: Urine Collection and Analysismentioning

confidence: 99%

Effect of curcumin in the prevention of experimentally induced nephrolithiasis in rats by ethylene glycol and Vitamin D3

Gandhi¹,

Balaraman²

2009

Oriental Pharmacy and Experimental Medicine

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“…Recently, sodium copper(II) chlorophyllin has been applied to pharmaceuticals for stomach and the epithelium [2] and as an inhibitor for oral oder [3], urinary odor [4] and the crystallisation of calcium oxalate stones in urine [5] owing to its antioxidative [6] and antimutagenic [71 properties. Pheophytin is fluorescent on exposed to light, but metallochlorophylls have no fluorescence when the central metal ions are paramagnetic.…”

Section: Metallochlorophyllsmentioning

confidence: 99%

Determination of Zinc(ii) Chlorophylls and Their Derivatives by High Performance Liquid Chromatography With Fluorometric Detection

Yamashita¹,

Inoue²

1991

Anal. Sci.

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Abstract-An HPLC method with fluorometric detection was developed for the determination of zinc (II) chlorophyll a and b ( Zn-chl-a, b ) and zinc(II) chlorophyll ins ( Zn-chins ). The analytical values by the HPLC method with UV and fluorometric detection were compared with those obtained by the spectrophotometric method. The values obtained by the HPLC method using a fluorometric detector were the most reproducible of three analytical methods. It was confirmed that the interferences from the absorption of coexistent pigments are removed by separation using HPLC and the overlapping of peaks from other metallochlorophylls is avoided by using a fluorescence spectromonitor. chlorin e6 ( Zn-chl-e6 ) and zinc(II) rhodin g7 ( Zn-rho-g7 ). The good separation was achieved with C18-bonded silica column and a mobile phase of acetone-methanol for Zn-chls and of methanol-water for Zn-chins. Tailing of peaks was observed for Zn-chlns due to dissosiation of protons in the carboxylic acid of these metallochlorophyllins.The pH value of the mobile phase was adjusted to 3.2 with acetic acid to prevent the release of protons in carboxyl groups. Under these HPLC conditions, Zn-chls and Zn-chins in unknown samples were determined by reversed-phase HPLC with fluorometric detection. EXPERIMENTAL Reagents and apparatusAll chemicals were of analytical-reagent grade and used as supplied without further purification. Organic solvents were purchased from Kanto Chemical Co. Inc. and of LC quality.The semi-preparative HPLC is a liquid chromatograph (Yanaco) equipped with a Develosil ODE column (36 cm x2.4 cm i.d., Nomura Chemical Co. ,Aichi), a VL-611 sample injector (1000mm3 loop, Jasco) and an M-315 (Shodex) variable wavelength detector. The flow rate of the mobile phase for Znchls, acetone-methanol, was 8.0 cm3min-'. The flow rate of the mobile phase for Zn-chins, methanol-H2O containing acetic acid (1% v.), was 3.0 cm3min' . The analytical HPLC instrumentation consisted of a Yanaco L-4000W pump, a Rheodyne 7120 injector with a lOOmm3 injection loop and a Shodex M-315 variable wavelength detector or a Shimadzu RF -530 fluorescence spectromonitor. A prepacked column purchased from GL Sciences was Inertsil ODS-2 which was reversed phase, 5-,u m spherical, octadesil silica in a 250 mm x4.6 mm i.d. column. The column temperature was set at 35 °C by dipping in a thermostat and the flow rate was 1.4 cm3min-' for Zn-chls and 1.0 cm3min' for Zn-chlns. Solvents used as a mobile phase were degassed every time before use by ultrasonically vibrating their containers.Absorption spectra were recorded with a U-2000 spectrophotometer (Hitachi) using 1-cm quartz cells. Preparation of zinc(II) chioro h llsThe preparation of Pheophytin a and b was described in the previous paper [8].Zinc(II) chlorophylls ( Zn-chls ) were prepared by refluxing a mixture of pheophytins dissolved in chloroform and zinc(II) acetate dissolved in methanol at 60 °C for 30 min. All operations were carried out in the dark in a nitrogen atmosphere. The formation and purity of Zn-c...

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“…Recent studies of calcium oxalate crystallization in a batch system are investigating, inter alia, the amount of water of crystallization, depending on the supersaturation ratio. , It could be deduced that calcium oxalate monohydrate (COM) or whewellite was the predominant crystal system . Furthermore, different promoters, inhibitors, , or processes were investigated in the production of calcium oxalate. , …”

Section: Introductionmentioning

confidence: 99%

“…22,23 It could be deduced that calcium oxalate monohydrate (COM) or whewellite was the predominant crystal system. 24 Furthermore, different promoters, 25 inhibitors, 26,27 or processes were investigated in the production of calcium oxalate. 28 Our aim is to control the crystallization of calcium oxalate and to obtain heterogeneous fragments which can be used as reference materials for kidney stone calculi.…”

Section: Introductionmentioning

confidence: 99%

Calcium Oxalate Crystallization: Influence of pH, Energy Input, and Supersaturation Ratio on the Synthesis of Artificial Kidney Stones

et al. 2021

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The removal of kidney stones can lead to small residual fragments remaining in the human body. Residual stone fragments can act as seeds for kidney stone crystallization and may necessitate another intervention. Therefore, it is important to create a consistent model with a particle size comparable to the range of kidney stone fragments. Thus, the size-determining parameters such as supersaturation ratio, energy input, and pH value are examined. The batch crystallizations were performed with supersaturation ratios between 5.07 and 6.12. The compositions of the dried samples were analyzed with Raman spectroscopy, infrared spectroscopy, and X-ray diffraction (XRD). The samples were identified as calcium oxalate monohydrate with spectroscopic analysis, while calcium oxalate dihydrate being the most prominent crystalline species at all supersaturation ratios for the investigated conditions. The aggregate size, obtained with analytical centrifugation, varied between 2.9 and 4.3 μm, while the crystallite domain size, obtained from XRD, varied from 40 to 61 nm. Our results indicate that particle sizes increase with increasing supersaturation, energy input, and pH. All syntheses yield a high particle heterogeneity and represent an ideal basis for reference materials of small kidney stone fragments. These results will help better understand and control the crystallization of calcium oxalate and the aggregation of such pseudopolymorphs.

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Crystallisation of calcium oxalate dihydrate in normal urine in presence of sodium copper chlorophyllin

Cited by 11 publications

References 8 publications

Effect of curcumin in the prevention of experimentally induced nephrolithiasis in rats by ethylene glycol and Vitamin D3

Effect of curcumin in the prevention of experimentally induced nephrolithiasis in rats by ethylene glycol and Vitamin D3

Determination of Zinc(ii) Chlorophylls and Their Derivatives by High Performance Liquid Chromatography With Fluorometric Detection

Calcium Oxalate Crystallization: Influence of pH, Energy Input, and Supersaturation Ratio on the Synthesis of Artificial Kidney Stones

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