In the present study, Copper nanoparticles (Cu-NPs) are produced using Nerium Oleander leaf extract via a green process. The prepared samples were examined by powder‒X-ray diffraction (P-XRD), ultraviolet-visible (UV-Vis) spectroscopy; Fourier transforms infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM) and antibacterial screenings. According to XRD measurements, the Cu-NPs have a monoclinic form. According to UV-Vis study, the minimal absorbance between 250 and 1000 nm is due to by the formation of high-quality samples. The functional groups of Cu-NPs were verified by FT-IR spectroscopy. According to the FE-SEM analysis, the Cu-NPs are clusters of smaller cylindrical objects with good uniformity. The antibacterial results show that the samples are fairly effective against Escherichia coli and Salmonella typhi bacterium. The findings indicate that Cu-NPs can be used in the medical sector for surgical tools.