Crystallization and preliminary study of endoglucanase (EGIII) from<i>Trichoderma reesei</i>

A Trichoderma reesei cDNA encoding a previously unknown protein with a C-terminal cellulosebinding domain was obtained by complementation screening of a 7: reesei cDNA library in a secl yeast mutant impaired in protein secretion. The 7: reesei protein shows amino acid similarity over its entire length to the Agaricus hisporus cellulose-induced protein CELl whose function is not known. These two proteins form a new glycosyl hydrolase family, number 61. Expression of the 7: reesei cDNA in yeast showed that it encoded a protein with endoglucanase activity and thus the protein was named EGIV and the corresponding gene eg14. Polyclonal antibodies were prepared against EGIV produced in Escherichia c-oli and detected a 56-kDa protein in the 7: reesei culture supernatant. Northern hybridisation revealed that 7: reesei eg14 is regulated in the same manner as other cellulase genes of this fungus.

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cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast

Saloheimo

Nakari-Setälä

Tenkanen

et al. 1997

European Journal of Biochemistry

170

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Crystallization and preliminary study of endoglucanase (EGIII) fromTrichoderma reesei

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cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast

cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast

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