2008
DOI: 10.1107/s1744309108014723
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Crystallization and preliminary X-ray diffraction experiments of arylmalonate decarboxylase fromAlcaligenes bronchisepticus

Abstract: Arylmalonate decarboxylase catalyses the enantioselective decarboxylation of -aryl--methylmalonates to produce optically pure -arylpropionates. The enzyme was crystallized with ammonium sulfate under alkaline pH conditions with the aim of understanding the mechanism of the enantioselective reaction. X-ray diffraction data collected to a resolution of 3.0 Å at cryogenic temperature showed that the crystals belonged to the orthorhombic space group P2 1 2 1 2 1 , with unit-cell parameters a = 83.13, b = 99.62, c … Show more

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Cited by 3 publications
(2 citation statements)
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“…A Hammett study12 of the reaction found a ρ value of +1.19, which is consistent with a negatively charged transition state 10. Recently, preliminary X-ray diffraction experiments and an X-ray crystal structure of AMDase were reported 13,14…”
Section: Enantioselective Protonation In Enzymatic Systemssupporting
confidence: 55%
“…A Hammett study12 of the reaction found a ρ value of +1.19, which is consistent with a negatively charged transition state 10. Recently, preliminary X-ray diffraction experiments and an X-ray crystal structure of AMDase were reported 13,14…”
Section: Enantioselective Protonation In Enzymatic Systemssupporting
confidence: 55%
“…Of the enzymatic approaches available, arylmalonate decarboxylases (AMDases) represent a direct way to access enantiopure α-aryl propionic acids from the corresponding malonic acids with only carbon dioxide as a byproduct (Figure A). One of the most well studied AMDases is from Bordetella bronchiseptica, which was first reported by Miyamoto and Ohta and subsequently investigated in terms of enzyme structure, , mechanism, engineering, and substrate scope. This enzyme has high selectivity to form R -configured products; however, one of the key challenges in this approach is the preparation of the required malonic acid substrates which are prone to spontaneous decarboxylation to give racemic propionic acids. Therefore, it is crucial to minimize this spontaneous process to be able to generate desired product in high enantiomeric excess.…”
Section: Introductionmentioning
confidence: 99%