Crystallization and Preliminary X-ray Diffraction Studies of β-Cryptogein, a Toxic Elicitin Secreted by the Phytopathogenic Fungus Phytophthora cryptogea

Crystal structure of a fungal elicitor secreted by Phytophthora cryptogea, a member of a novel class of plant necrotic proteins

“…CRY crystallizes in the space group P4 1 22 with cell dimensions a = b = 46.5 Å, c = 134.9 Å [16]. An extensive search for heavy-atom derivatives yielded only one, a poorly phasing platinum derivative.…”

“…CRY was crystallized in concentrated sodium chloride as previously described by Guilloteau et al [16]. Use of macroseeding procedures made it possible to systematically grow well shaped and sufficiently large crystals overnight through the pH range 4.0 to 10.0, while eliminating twinning which is frequent in this crystalline form.…”