Crystallization for the Downstream Processing of Proteins

Developing the biofacility of the future based on continuous processing and single-use technology

“…Consequently, alternative UO for the capture step or intermediate purification/polishing like aqueous two phase extraction (Muendges et al, 2015;Rosa et al, 2011;Schwienheer et al, 2015), precipitation (Sim et al, 2012;Hammerschmidt et al, 2014) or crystallization (Schmidt et al, 2005) have not been taken into account. Although, these methods might have advantages regarding continuous operation mode and process economics, they do not fulfill the requirement of being a platform technology in antibody production.…”

Future of antibody purification

“…Another approach used in low cost industrial enzymes is crystallization, although its use as a unit operation for therapeutic protein purification has been limited [134][135][136], primarily due to the difficulty of crystallization from impure process streams and scalability. Crystallization in protein formulation can be more readily envisioned [137], and though still limited in its application, has provided the impetus for development of large-scale crystallization processes.…”

Downstream Processing in Industrial Biotechnology

“…Even in the case of protein products, crystallization, which was originally limited to determination of their three -dimensional structure, has now been extended to its application as a downstream processing technique for recovery directly from relatively impure solutions and even from fermentation broths [28] . The possibility of replacing competing methods such as chromatography with a relatively simple and inexpensive technique makes crystallization an attractive purifi cation technique, with further advantages of formulation and storage.…”