Crystallization, Luminescence and Cytocompatibility of Hexagonal Calcium Doped Terbium Phosphate Hydrate Nanoparticles

Gómez‐Morales, Jaime; Fernández-Penas, Raquel; Romero‐Castillo, Ismael; Verdugo‐Escamilla, Cristóbal; Choquesillo-Lazarte, Duane; D’Urso, Annarita; Prat, María

doi:10.3390/nano11020322

Cited by 13 publications

(8 citation statements)

References 54 publications

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“…These NPs were cytocompabible after 7 days of incubation also on human osteoblasts, both primary, which were obtained from explants of human trabecular bone fragments, and differentiated with an appropriate protocol from human mesenchymal stem cells. These data are in line with previous ones, reporting the properties of other lanthanide-doped nanocrystals [ 27 , 69 ]. The absence of toxicity after long incubation time allowed us to do experiments lasting up to 5 days to assess the effects of the DF loaded on Tb-doped NPs.…”

Section: Discussionsupporting

confidence: 93%

“…The presence of carbonate (CO 3 2− ) is attested by the small band at 873 cm − 1 (υ 2 CO 3 ) and υ 3 CO 3 mode, with bands ~1417 cm − 1 and 1468 cm −1 . The band at ~1590 cm −1 observed in both samples, is ascribed to the antisymmetric stretching frequencies of the carboxylate groups of the citrate, indicating the citrate molecules are adsorbed on the apatite surface, as previously reported [ 27 ]. On the other hand, the complementary characterization by Raman ( Figure S2b ) reveals the main band at 960 cm −1 which corresponds to the antisymmetric vibration mode of phosphate groups (υ 1 PO 4 ) of the apatite phase [ 45 ].…”

Section: Resultssupporting

confidence: 82%

“… TEM micrographs of cAp ( a ) and cAp-Tb NPs ( b ) prepared by thermal decomplexing method [ 27 , 29 ]. …”

Section: Figurementioning

confidence: 99%

“…The apatite surfaces can be modified (charged to improve the interactions with living cells, particularly the osteoblasts) [ 19 ]. In addition, Ap NPs have already been shown to be valuable nanocarriers for different types of molecules [ 20 ], including nucleic acids [ 21 ], proteins [ 22 ], antibiotics [ 3 ], chemotherapeutics [ 23 , 24 ], fluorophores [ 25 ], and luminescent moieties [ 26 , 27 ]. Among their advantageous properties are high biocompatibility, good biodegradability, high loading capacity with the ability to bind moieties through both surface calcium and phosphate groups by isothermal adsorption.…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

et al. 2022

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Luminescent nanoparticles are innovative tools for medicine, allowing the imaging of cells and tissues, and, at the same time, carrying and releasing different types of molecules. We explored and compared the loading/release ability of diclofenac (COX-2 antagonist), in both undoped- and luminescent Terbium3+ (Tb3+)-doped citrate-coated carbonated apatite nanoparticles at different temperatures (25, 37, 40 °C) and pHs (7.4, 5.2). The cytocompatibility was evaluated on two osteosarcoma cell lines and primary human osteoblasts. Biological effects of diclofenac-loaded-nanoparticles were monitored in an in vitro osteoblast’s cytokine–induced inflammation model by evaluating COX-2 mRNA expression and production of PGE2. Adsorption isotherms fitted the multilayer Langmuir-Freundlich model. The maximum adsorbed amounts at 37 °C were higher than at 25 °C, and particularly when using the Tb3+ -doped particles. Diclofenac-release efficiencies were higher at pH 5.2, a condition simulating a local inflammation. The luminescence properties of diclofenac-loaded Tb3+ -doped particles were affected by pH, being the relative luminescence intensity higher at pH 5.2 and the luminescence lifetime higher at pH 7.4, but not influenced either by the temperature or by the diclofenac-loaded amount. Both undoped and Tb3+-doped nanoparticles were cytocompatible. In addition, diclofenac release increased COX-2 expression and decreased PGE2 production in an in vitro inflammation model. These findings evidence the potential of these nanoparticles for osteo-localized delivery of anti-inflammatory drugs and the possibility to localize the inflammation, characterized by a decrease in pH, by changes in luminescence.

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Section: Discussionsupporting

confidence: 93%

Section: Resultssupporting

confidence: 82%

“… TEM micrographs of cAp ( a ) and cAp-Tb NPs ( b ) prepared by thermal decomplexing method [ 27 , 29 ]. …”

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

et al. 2022

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“…10 A549 cells contain multilamellar cytoplasmic inclusion bodies typical of those found in type II alveolar epithelial cells. 10 A549 cells have been widely used in reports estimating pulmonary toxicity of NMs, eg silica (SiO 2 ), iron oxides (Fe x O y ), zinc oxide (ZnO), titanium dioxide (TiO 2 ), [11][12][13] silver (Ag) 14 nanoparticles and other specifically functionalized nanoparticles, 15 nanofibers, 16,17 nanosheets 18 and carbon-based nanomaterials. 19,20 A variety of TiO 2 NMs, eg nanoparticles, 21 nanofibers 17 and nanotubes, 22 has been developed.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Báčová¹,

Knotek²,

Kopecká³

et al. 2022

IJN

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Purpose Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO 2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO 2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO 2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO 2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO 2 P25 for biological testing in A549 cells and to evaluate their biological effects. Methods We determined the size and crystallinity of TiO 2 P25. We used four techniques for TiO 2 P25 dispersion. We estimated the colloid stability of TiO 2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO 2 P25 (0–100 µg.mL −1 ) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy. Results We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO 2 P25. Under these conditions, TiO 2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO 2 P25 in A549 cells were met, similar results on the biological effects of TiO 2 P25 were obtained in two independent cell laboratories. Conclusion We optimized the experimental conditions of TiO 2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO 2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO 2 P25 as a reference material.

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Divalent copper ions-doped strontium magnesium phosphate nanopowder: synthesis and characterization

Rajendrakumar,

Anjaneyulu,

Vasu

et al. 2023

J Mater Sci: Mater Electron

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Crystallization, Luminescence and Cytocompatibility of Hexagonal Calcium Doped Terbium Phosphate Hydrate Nanoparticles

Cited by 13 publications

References 54 publications

Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Divalent copper ions-doped strontium magnesium phosphate nanopowder: synthesis and characterization

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