Crystallization of lysozyme from lysozyme – ovalbumin mixtures: Separation potential and crystal growth kinetics

Study on the influence of lysozyme crystallization conditions on crystal properties in crystallizers of varied sizes when temperature is the manipulated variable

“…Similar observation has also been reported in solvent freeze-out method in protein crystallization, despite the encouraging results obtained from lysozyme purified from lysozyme-ovalbumin mixture, as reported by Diaz Borbon and Ulrich [20]. Maosoongnern et al, [21] conducted seeded isothermal crystallization of lysozyme-ovalbumin mixture in a 100-ml vessel for 24 h and obtained comparable purification results with a yield of 80%. Protein crystallization based on gas diffusion has also been investigated [22][23][24]; it is however considerably difficult to control and difficult to achieve process scale-up.…”

Improving the reproducibility of size distribution of protein crystals produced in continuous slug flow crystallizer operated at short residence time

“…Anything but chromatography (ABC) methodologies, despite being argued to be changed to "Anything and Chromatography" (AAC) [78], has been widely investigated in the DSP of biopharmaceuticals [7,69]. Currently, membrane-based technology [77,[79][80][81], aqueous two-phase system (ATPS) [69,[82][83][84][85][86], magnetic separation [12,[87][88][89][90], precipitation [6,87,[91][92][93][94], and crystallization [6,26,38,84,[95][96][97][98] provide promising alternatives. Besides, to enhance the processing flexibility, better respond to fluctuating market demands, continuous manufacturing strategies have been sought as an effort to achieve process intensification in biologics manufacturing [11,70,[99][100][101][102].…”

“…Specifically, the nucleation and crystal growth of Van might be affected, which will ultimately result in change of the yield, crystal size, and crystal shape. In fact, several studies have investigated the effect of impurities on the batch crystallization of proteins [97,148,[353][354][355][356], and this is a promising future research direction for us to explore.…”

Protein crystal occurrence domains in selective protein crystallisation for bio-separation

“…Judge et al studied protein crystallisation in the presence of protein impurities, 23,34 and preferential separation of lysozyme from lysozyme-ovalbumin mixtures was achieved by seeded batch crystallisation. 35 Nonetheless, in the above cases, only a limited range of mixture composition was covered and only one of the proteins in the solution could be crystallised out under the solution conditions. Systematic knowledge of the crystallisation behaviour of the target protein from the mixture is still absent.…”