Crystallization of the effector-binding domains of BenM and CatM, LysR-type transcriptional regulators from<i>Acinetobacter</i>sp. ADP1

Clark, Todd J.; Haddad, Sandra; Neidle, Ellen L.; Momany, Cory

doi:10.1107/s0907444903021589

Cited by 19 publications

(25 citation statements)

References 21 publications

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“…It is also possible that the two CatM substitutions affect transcription by different mechanisms. More information about effector-induced conformational changes in CatM and its variants should be provided by current structural studies (7,9). Structural investigations, which may also reveal the basis for the inability of CatM to respond to benzoate, will complement the physiological characterization of the regulatory mutations in this report.…”

Section: Discussionmentioning

confidence: 99%

CatM Regulation of the benABCDE Operon: Functional Divergence of Two LysR-Type Paralogs in Acinetobacter baylyi ADP1

Ezezika

Collier-Hyams

Dale

et al. 2006

Appl Environ Microbiol

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Two LysR-type transcriptional regulators, BenM and CatM, control benzoate consumption by the soil bacterium Acinetobacter baylyi ADP1. These homologs play overlapping roles in the expression of multiple genes. This study focuses on the benABCDE operon, which initiates benzoate catabolism. At this locus, BenM and CatM each activate transcription in response to the catabolite cis,cis-muconate. BenM, but not CatM, additionally responds to benzoate as an effector. Regulation by CatM alone is insufficient for growth on benzoate as the sole carbon source. However, three point mutations independently increased CatM-activated benA transcription and enabled growth on benzoate without BenM. Two mutations generate variants with one amino acid change in the 303-residue CatM, CatM(V158M) and CatM(R156H). These substitutions affected regulation of benA differently than that of catB, another CatM-regulated gene involved in benzoate catabolism. In relation to CatM, CatM(V158M) increased cis,cis-muconate-dependent transcription of benA but decreased that of catB. CatM(R156H) increased effector-independent expression of catB compared to CatM. In contrast, cis,cis-muconate was required with CatM(R156H) to activate unusually high benA expression. Thus, induction by cis,cis-muconate depends on both the sequence of CatM and the promoter. A point mutation at position ؊40 of the benA promoter enhanced CatM-activated gene expression and altered regulation by CatM(R156H). BenM and CatM bound to the same locations on ben region DNA. The frequency with which spontaneous mutations allow CatM to substitute for BenM might predict that one regulator would be sufficient for controlling benzoate consumption. This prediction is discussed in light of current and previous studies of the BenM-CatM regulon.

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Section: Discussionmentioning

confidence: 99%

CatM Regulation of the benABCDE Operon: Functional Divergence of Two LysR-Type Paralogs in Acinetobacter baylyi ADP1

Ezezika

Collier-Hyams

Dale

et al. 2006

Appl Environ Microbiol

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“…Crystals of BenM and CatM devoid of their N-terminal DNA binding domain have also been obtained, but their structures have not been resolved yet (29). CbnR crystallized as a tetramer (Fig.…”

Section: Structure and Conformationmentioning

confidence: 99%

Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds

Tropel

Meer

2004

Microbiol Mol Biol Rev

345

338

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Human activities have resulted in the release and introduction into the environment of a plethora of aromatic chemicals. The interest in discovering how bacteria are dealing with hazardous environmental pollutants has driven a large research community and has resulted in important biochemical, genetic, and physiological knowledge about the degradation capacities of microorganisms and their application in bioremediation, green chemistry, or production of pharmacy synthons. In addition, regulation of catabolic pathway expression has attracted the interest of numerous different groups, and several catabolic pathway regulators have been exemplary for understanding transcription control mechanisms. More recently, information about regulatory systems has been used to construct whole-cell living bioreporters that are used to measure the quality of the aqueous, soil, and air environment. The topic of biodegradation is relatively coherent, and this review presents a coherent overview of the regulatory systems involved in the transcriptional control of catabolic pathways. This review summarizes the different regulatory systems involved in biodegradation pathways of aromatic compounds linking them to other known protein families. Specific attention has been paid to describing the genetic organization of the regulatory genes, promoters, and target operon(s) and to discussing present knowledge about signaling molecules, DNA binding properties, and operator characteristics, and evidence from regulatory mutants. For each regulator family, this information is combined with recently obtained protein structural information to arrive at a possible mechanism of transcription activation. This demonstrates the diversity of control mechanisms existing in catabolic pathways

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“…CatM was initially identified as a repressor of the catBCIJFD operon encoding proteins required to convert benzoate into tricarboxylic acid cycle intermediates (Romero-Arroyo et al, 1995;Collier et al, 1998;Clark et al, 2003). It was later reclassified as a transcriptional activator and found to be part of a more complex regulatory network involving BenM.…”

Section: Lysr Family Transcriptional Regulatorsmentioning

confidence: 99%

Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins

2008

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The LysR family of transcriptional regulators represents the most abundant type of transcriptional regulator in the prokaryotic kingdom. Members of this family have a conserved structure with an Nterminal DNA-binding helix-turn-helix motif and a C-terminal co-inducer-binding domain. Despite considerable conservation both structurally and functionally, LysR-type transcriptional regulators (LTTRs) regulate a diverse set of genes, including those involved in virulence, metabolism, quorum sensing and motility. Numerous structural and transcriptional studies of members of the LTTR family are helping to unravel a compelling paradigm that has evolved from the original observations and conclusions that were made about this family of transcriptional regulators.

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Crystallization of the effector-binding domains of BenM and CatM, LysR-type transcriptional regulators fromAcinetobactersp. ADP1

Cited by 19 publications

References 21 publications

CatM Regulation of the benABCDE Operon: Functional Divergence of Two LysR-Type Paralogs in Acinetobacter baylyi ADP1

CatM Regulation of the benABCDE Operon: Functional Divergence of Two LysR-Type Paralogs in Acinetobacter baylyi ADP1

Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds

Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins

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