CSF flow cytometry greatly improves diagnostic accuracy in CNS hematologic malignancies

Abstract

The diagnostic value of flow cytometry is more than twice that of cytomorphology. However, cytomorphologic examination of the CSF has additional diagnostic and possibly prognostic value, and should still be performed in conjunction with flow cytometry.

Cerebrospinal fluid chimerism analysis in patients with neurological symptoms after allogeneic cell transplantation

“…15 Several reports have compared cytology with FC showing a broad range of concordant results. 4,9,10 Our FC findings and chimerism analysis in the group of patients relapsing in the CNS showed a high concordance degree. With the exception of three patients, agreement was observed between FC and chimerism analysis in 11 patients.…”

“…Characterization of CSF cells using flow cytometry (FC) improved the detection of malignant and non-malignant cells and constitutes a useful diagnostic complement. 4 The reported use of molecular biology techniques in CSF cells in patients undergoing alloHCT is scarce, and these reports mostly refer to the pediatric population involving small patient cohorts. 5,6 Donor-recipient chimerism routinely used to evaluate hematopoietic chimerism following alloHCT usually does not constitute a part of the CSF analysis in patients with neurological symptoms.…”

“…7,8 Furthermore, whereas several reports compare cytology with FC in the identification of CSF cells, there is no comparison between FC and chimerism analysis in this type of sample. 4,9,10 In this study, we evaluated the potential contribution of CSF donor-recipient chimerism analysis in patients with neurological symptoms following alloHCT. …”

Presentaciones orales

“…Además, en el caso de linfomas no Hodgkin B agresivos, se ha descrito (10) que el punto de corte entre los casos en los que la citología convencional resulta positiva o sugerente de infiltración y aquéllos en los que es negativa, se situaría en una infiltración por células B neoplásicas superior a 20% del número global de células o de una o más células B neoplásicas por µl, lo cual apoya la utilización de la citometría de flujo como técnica de elección para el estudio de infiltración neoplásica en muestras con escaso número de células (2,3,7,11).…”

Detection and outcome of occult leptomeningeal disease in diffuse large B-cell lymphoma and Burkitt lymphoma

“…5,6 Several studies now show that CSF flow cytometry (FCM), which is significantly more sensitive than cytology, detects a relatively high rate of occult disease. 4,7,8 The ability to detect occult leptomeningeal involvement early in the disease raises uncertainties regarding optimal treatment. FCM provides an opportunity to 'prospectively' identify patients with leptomeningeal disease, thereby enabling the assessment of intrathecal chemotherapy on CNS recurrence and survival, which is an area of controversy.…”