CsMYC2 is involved in the regulation of phenylpropanoid biosynthesis induced by trypsin in cucumber (Cucumis sativus) during storage

Wang, Jie; Tian, Pingping; Sun, Jiaju; Li, Bairu; Jia, Jingyu; Yuan, Jin-Peng; Li, Xin; Gu, Shaobin; Pang, Xinyue

doi:10.1016/j.plaphy.2023.01.041

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…By conducting GSEA analysis on the 83 genes enriched in the MAPK signaling pathway, the results indicated that in addition to being annotated in the MAPK pathway itself (MAP04016), they were also annotated in the Plant-Pathogen Interaction pathway (MAP04626) and Plant Hormone Signal Transduction pathway (MAP04075) (Table 1). A total of 9 genes were enriched in the leading edge subset of the MAP04075 pathway, among which the screenedMYCtranscription factor (Table S3) has been previously reported by our laboratory [29]. Moreover, from the leading edge subset of the MAP04626 pathway, a total of 8 genes were screened (Table S4).…”

Section: Gsea Analysis Of Mapk Pathway-related Genesmentioning

confidence: 97%

“…The samples were placed at 25°C. Every 24 hours, observations, photographs, records, and measurements of water loss rates were conducted [29]. After one week of storage, the C. sativus epidermis that had undergone treatment was excised using a surgical blade, mixed thoroughly, and rapidly placed in liquid nitrogen for 3-5 minutes.…”

Section: Experimental Materials and Reagentsmentioning

confidence: 99%

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CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

Sun,

wang,

Chen

et al. 2023

Preprint

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Background The Mitogen-Activated Protein Kinase (MAPK) cascade pathway extensively participates in the physiological regulation processes of plants. Trypsin, as an inducer of resistance, significantly enhanced the storage quality of Cucumis sativus (C. sativus). However, the mechanism by which trypsin regulated the synthesis of phenylpropanoid compounds through the MAPK pathway to influence fruit resistance remained to be elucidated. Methods To investigate the molecular mechanism underlying trypsin-induced C. sativus resistance, we conducted a combined transcriptomic and widely targeted metabolomic analysis, validated through Virus-Induced Gene Silencing (VIGS). Results Transcriptomic results revealed that a total of 83 differentially expressed genes (DEGs)were enriched in the MAPK pathway, with 48 genes significantly downregulated and 35 genes significantly upregulated. GSEA analysis further identified the WRKY33 transcription factor from the leading edge subset. Bioinformatics analysis indicated that CsWRKY33 shared high homology with WRKY22 in Arabidopsis. The combined analysis of transcriptomics and widely targeted metabolomics demonstrated significant upregulation in the synthesis of compounds such as vanillin, dihydrocharcone-4'-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid. Co-expression network analysis showed that these key metabolites were negatively regulated by CsWRKY33. VIGS results showed that silencing CsWRKY33 enhanced fruit resistance and extended storage time. Conclusion This study revealed that trypsin could downregulate the expression of CsWRKY33 and promote the synthesis of compounds with high antioxidant and antibacterial activity, such as vanillin, dihydrocharcone-4'-O-glucoside, and 2-hydroxy-3-phenylpropanoic acid. This enhancement led to increasing fruit disease resistance and delayed senescence and decay.

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Section: Gsea Analysis Of Mapk Pathway-related Genesmentioning

confidence: 97%

Section: Experimental Materials and Reagentsmentioning

confidence: 99%

CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

Sun,

wang,

Chen

et al. 2023

Preprint

Self Cite

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“…The FPKM values for genes and the peak values of metabolites were used as a matrix for Pearson's partial correlation analysis [53]. Through O2PLS analysis, the internal connection between the two omics data was mined, the degree of correlation between the two omics data was determined, and the hub genes and metabolites that cause this correlation were determined [54,55].…”

Section: Integrated Metabolomic and Transcriptomic Analysesmentioning

confidence: 99%

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

Tian,

Liu,

Chen

et al. 2024

IJMS

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As a highly economic berry fruit crop, blueberry is enjoyed by most people and has various potential health benefits, many of which are attributed to the relatively high concentrations of flavonoids. To obtain more accurate and comprehensive transcripts, the full-length transcriptome of half-highbush blueberry (Vaccinium corymbosum/angustifolium cultivar Northland) obtained using single molecule real-time and next-generation sequencing technologies was reported for the first time. Overall, 147,569 consensus transcripts (average length, 2738 bp; N50, 3176 bp) were obtained. After quality control steps, 63,425 high-quality isoforms were obtained and 5030 novel genes, 3002 long non-coding RNAs, 3946 transcription factor genes (TFs), 30,540 alternative splicing events, and 2285 fusion gene pairs were identified. To better explore the molecular mechanism of flavonoid biosynthesis in mature blueberry fruit, an integrative analysis of the metabolome and transcriptome was performed on the exocarp, sarcocarp, and seed. A relatively complete biosynthesis pathway map of phenylpropanoids, flavonoids, and proanthocyanins in blueberry was constructed. The results of the joint analysis showed that the 228 functional genes and 42 TFs regulated 78 differentially expressed metabolites within the biosynthesis pathway of phenylpropanoids/flavonoids. O2PLS analysis results showed that the key metabolites differentially accumulated in blueberry fruit tissues were albireodelphin, delphinidin 3,5-diglucoside, delphinidin 3-O-rutinoside, and delphinidin 3-O-sophoroside, and 10 structural genes (4 Vc4CLs, 3 VcBZ1s, 1 VcUGT75C1, 1 VcAT, and 1 VcUGAT), 4 transporter genes (1 VcGSTF and 3 VcMATEs), and 10 TFs (1 VcMYB, 2 VcbHLHs, 4 VcWD40s, and 3 VcNACs) exhibited strong correlations with 4 delphinidin glycosides. These findings provide insights into the molecular mechanisms of flavonoid biosynthesis and accumulation in blueberry fruit.

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A comprehensive physiological and -Omic analysis of trypsin-mediated protection of green pepper fruits from chilling injury

Zhao,

Wang,

Yue

et al. 2024

Food Chemistry

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CsMYC2 is involved in the regulation of phenylpropanoid biosynthesis induced by trypsin in cucumber (Cucumis sativus) during storage

Cited by 5 publications

References 41 publications

CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

CsWRKY33: A Crucial Transcription Factor in the MAPK Pathway Inhibited by Trypsin, Participating in the Suppression of Cucumis sativus Senescence

Integrated Untargeted Metabolome, Full-Length Sequencing and Transcriptome Analyses Reveal the Mechanism of Flavonoid Biosynthesis in Blueberry (Vaccinium spp.) Fruit

A comprehensive physiological and -Omic analysis of trypsin-mediated protection of green pepper fruits from chilling injury

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