CspC and CspD are essential for Caulobacter crescentus stationary phase survival

Balhesteros, Heloise; Mazzon, Ricardo Ruiz; Silva, Carolina A. P. T. da; Lang, Elza A. S.; Marques, Marilis V.

doi:10.1007/s00203-010-0602-8

Cited by 18 publications

(23 citation statements)

References 49 publications

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“…The cspA, cspC, and cspD mutant strains were constructed previously (4,31). cspB mutant and cspA cspB double mutant strains were obtained by allelic replacement using the NA1000 and cspA mutant strains, respectively, as background.…”

Section: Methodsmentioning

confidence: 99%

“…The C. crescentus genome contains four paralogues of csp genes, two of them (cspA and cspB) being cold induced, encoding proteins containing a single cold shock domain (CSD), and the other two (cspC and cspD) being stationary phase induced, encoding proteins with two CSDs (4,31). Recently, we demonstrated that stationary-phase induction of C. crescentus cspD is regulated by the levels of the second messenger ppGpp and by the SpdR/SpdS two-component system (7).…”

mentioning

confidence: 99%

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Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

et al. 2012

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Cold shock proteins (CSPs) are nucleic acid binding chaperones, first described as being induced to solve the problem of mRNA stabilization after temperature downshift. Caulobacter crescentus has four CSPs: CspA and CspB, which are cold induced, and CspC and CspD, which are induced only in stationary phase. In this work we have determined that the synthesis of both CspA and CspB reaches the maximum levels early in the acclimation phase. The deletion of cspA causes a decrease in growth at low temperature, whereas the strain with a deletion of cspB has a very subtle and transient cold-related growth phenotype. The cspA cspB double mutant has a slightly more severe phenotype than that of the cspA mutant, suggesting that although CspA may be more important to cold adaptation than CspB, both proteins have a role in this process. Gene expression analyses were carried out using cspA and cspB regulatory fusions to the lacZ reporter gene and showed that both genes are regulated at the transcriptional and posttranscriptional levels. Deletion mapping of the long 5=-untranslated region (5=-UTR) of each gene identified a common region important for cold induction, probably via translation enhancement. In contrast to what was reported for other bacteria, these cold shock genes have no regulatory regions downstream from ATG that are important for cold induction. This work shows that the importance of CspA and CspB to C. crescentus cold adaptation, mechanisms of regulation, and pattern of expression during the acclimation phase apparently differs in many aspects from what has been described so far for other bacteria.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

et al. 2012

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“…Phenotypic analyses of cspC and cspD null mutants, as well as the deletion of both genes, showed that cell viability is severely decreased in the cspC and cspCD mutants upon entry into stationary phase (3). The cspC cells show aberrant morphology at stationary phase, and this phenotype is more severe in the double mutant, suggesting that cspD can at least in part compensate for the lack of cspC (3).…”

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confidence: 99%

SpdR, a Response Regulator Required for Stationary-Phase Induction of Caulobacter crescentus cspD

et al. 2010

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The cold shock protein (CSP) family includes small polypeptides that are induced upon temperature downshift and stationary phase. The genome of the alphaproteobacterium Caulobacter crescentus encodes four CSPs, with two being induced by cold shock and two at the onset of stationary phase. In order to identify the environmental signals and cell factors that are involved in cspD expression at stationary phase, we have analyzed cspD transcription during growth under several nutrient conditions. The results showed that expression of cspD was affected by the medium composition and was inversely proportional to the growth rate. The maximum levels of expression were decreased in a spoT mutant, indicating that ppGpp may be involved in the signalization for carbon starvation induction of cspD. A Tn5 mutant library was screened for mutants with reduced cspD expression, and 10 clones that showed at least a 50% reduction in expression were identified. Among these, a strain with a transposon insertion into a response regulator of a two-component system showed no induction of cspD at stationary phase. This protein (SpdR) was able to acquire a phosphate group from its cognate histidine kinase, and gel mobility shift assay and DNase I footprinting experiments showed that it binds to an inverted repeat sequence of the cspD regulatory region. A mutated SpdR with a substitution of the conserved aspartyl residue that is the probable phosphorylation site is unable to bind to the cspD regulatory region and to complement the spdR mutant phenotype.

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“…This suggests that a mechanism must be in place to promote hypercurvature in stationary phase cells that is in addition to inhibition of division through reduced FtsZ levels. Caulobacter cells in stationary phase differentially regulate expression of many genes to promote survival and, perhaps, morphological changes through transcriptional regulators such as SpdR [98–100]. …”

Section: Shapeshifting During Environmental Stressmentioning

confidence: 99%

Shapeshifting to Survive: Shape Determination and Regulation in Caulobacter crescentus

Woldemeskel

Goley

2017

Trends in Microbiology

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Bacterial cell shape is a genetically encoded and inherited feature that is optimized for efficient growth, survival and propagation of bacteria. In addition, bacterial cell morphology is adaptable to changes in environmental conditions. Work in recent years has demonstrated that individual features of cell shape, such as length or curvature, arise through the spatial regulation of cell wall synthesis by cytoskeletal proteins. However, the mechanisms by which these different morphogenetic factors are coordinated and how they may be globally regulated in response to cell cycle and environmental cues are only beginning to emerge. Here, we have summarized recent advances that have been made to understand morphology in the dimorphic Gram-negative bacterium, Caulobacter crescentus.

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CspC and CspD are essential for Caulobacter crescentus stationary phase survival

Cited by 18 publications

References 49 publications

Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

Cold Shock Genes cspA and cspB from Caulobacter crescentus Are Posttranscriptionally Regulated and Important for Cold Adaptation

SpdR, a Response Regulator Required for Stationary-Phase Induction of Caulobacter crescentus cspD

Shapeshifting to Survive: Shape Determination and Regulation in Caulobacter crescentus

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