Since low-power Wi-Fi sensors are connected to the Internet, effective radio spectrum use is crucial for developing an efficient Medium Access Control (MAC) protocol for Wi-Fi sensor networks. A connectivity-based multipolling mechanism was employed for Access Points to grant uplink transmission opportunities to Wi-Fi nodes with a reduced number of multipolling frame transmissions. The existing connectivity-based multipolling mechanism in IEEE 802.11 wireless LANs with many nodes may require excessive time to derive the optimal number of serially connected sequences due to the backtracking algorithm based on the Traveling Salesman Problem model. This limitation hinders the real-time implementation of the connectivity-based multipolling mechanism in Wi-Fi sensor networks. In this study, an efficient node insertion algorithm is proposed, by which the number of derived serially connected multipolling sequences that cover nodes in Wi-Fi sensor networks converges to only one as the number of Wi-Fi sensors increases in Wi-Fi sensor networks. As verified by simulation experiments for Wi-Fi sensor networks, the proposed node insertion algorithm produces a near-optimal number of multipolling sequences that cover the nodes in Wi-Fi sensor networks. This study proposes a node insertion algorithm for the real-time implementation of the connectivity-based multipolling mechanism in MAC protocol for Wi-Fi sensor networks.