CTBP1 and metabolic syndrome induce an mRNA and miRNA expression profile critical for breast cancer progression and metastasis

Farré, Paula Lucía; Scalise, Georgina Daniela; Duca, Rocío Belén; Dalton, Guillermo Nicolás; Massillo, Cintia; Porretti, Juliana; Graña, Karen Daniela; Gardner, Kevin; Luca, Paola De

doi:10.18632/oncotarget.24486

Cited by 22 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of CTBP1 in EMT and metastasis has been stated before in hepatocellular carcinoma 20 and breast cancer 18,21 . More importantly, Wang et al 22 reported that mice injected through the tail vein with CTBP1-depleted PC3 cells had less metastasis development compared to control.…”

Section: Discussionmentioning

confidence: 63%

See 1 more Smart Citation

CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice

et al. 2019

Self Cite

View full text Add to dashboard Cite

About 20% of prostate cancer (PCa) patients progress to metastatic disease. Metabolic syndrome (MeS) is a pathophysiological disorder that increases PCa risk and aggressiveness. C-terminal binding protein (CTBP1) is a transcriptional corepressor that is activated by high-fat diet (HFD). Previously, our group established a MeS/PCa mice model that identified CTBP1 as a novel link associating both diseases. Here, we integrated in vitro (prostate tumor cell lines) and in vivo (MeS/PCa NSG mice) models with molecular and cell biology techniques to investigate MeS/CTBP1 impact over PCa progression, particularly over cell adhesion, mRNA/miRNA expression and PCa spontaneous metastasis development. We found that CTBP1/MeS regulated expression of genes relevant to cell adhesion and PCa progression, such as cadherins, integrins, connexins, and miRNAs in PC3 xenografts. CTBP1 diminished PCa cell adhesion, membrane attachment to substrate and increased filopodia number by modulating gene expression to favor a mesenchymal phenotype. NSG mice fed with HFD and inoculated with CTBP1-depleted PC3 cells, showed a decreased number and size of lung metastases compared to control. Finally, CTBP1 and HFD reduce hsa-mir-30b-5p plasma levels in mice. This study uncovers for the first time the role of CTBP1/MeS in PCa progression and its molecular targets.

show abstract

Section: Discussionmentioning

confidence: 63%

“…We previously reported that CTBP1 regulates miRNAs 15,17,18 . Concisely, RNA samples obtained from the xenografts tumors described above were used to hybridize a miRNA expression microarray (Affymetrix) 15 .…”

Section: Resultsmentioning

confidence: 99%

CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Of the eight miRNAs, only let-7e-3p and miR-1249-3p have been associated with breast cancer. Let-7e-3p has been identified as one of the miRNAs involved in breast cancer development that is regulated by C-terminal binding protein 1 (CTBP1) and metabolic syndrome [30]. Besides, let-7e-3p has been suggested to play a tumor suppressor role especially in estrogen receptor (ER)-negative or basal-like tumors [31], and low expression of let-7e-3p in breast cancer tissues has been associated with poor prognosis [31].…”

Section: Discussionmentioning

confidence: 99%

A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms

Loke

Munusamy

Koh

et al. 2019

Cancers

View full text Add to dashboard Cite

Although mammography is the gold standard for breast cancer screening, the high rates of false-positive mammograms remain a concern. Thus, there is an unmet clinical need for a non-invasive and reliable test to differentiate between malignant and benign breast lesions in order to avoid subjecting patients with abnormal mammograms to unnecessary follow-up diagnostic procedures. Serum samples from 116 malignant breast lesions and 64 benign breast lesions were comprehensively profiled for 2,083 microRNAs (miRNAs) using next-generation sequencing. Of the 180 samples profiled, three outliers were removed based on the principal component analysis (PCA), and the remaining samples were divided into training (n = 125) and test (n = 52) sets at a 70:30 ratio for further analysis. In the training set, significantly differentially expressed miRNAs (adjusted p < 0.01) were identified after correcting for multiple testing using a false discovery rate. Subsequently, a predictive classification model using an eight-miRNA signature and a Bayesian logistic regression algorithm was developed. Based on the receiver operating characteristic (ROC) curve analysis in the test set, the model could achieve an area under the curve (AUC) of 0.9542. Together, this study demonstrates the potential use of circulating miRNAs as an adjunct test to stratify breast lesions in patients with abnormal screening mammograms.

show abstract

“…MetS is an important risk factor for cardiovascular disease incidence and mortality [4] and chronic kidney disease progression [5,6]. Moreover, a previous study suggested that MetS is associated with an increased risk of breast cancer [7,8] and Parkinson’s disease [9]. MetS is a comprehensive condition caused by genetic predisposition and environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Association between Heavy Metals, Bisphenol A, Volatile Organic Compounds and Phthalates and Metabolic Syndrome

Shim

Ock

Kim

et al. 2019

IJERPH

View full text Add to dashboard Cite

The incidence of metabolic syndrome (MetS), which causes heart disease and stroke, has increased significantly worldwide. Although many studies have revealed the relationship between heavy metals (cadmium, mercury, and lead), the sum of metabolites of di(2-ethylhexyl) phthalate (DEHP), and MetS, the results remain inconsistent. No study has reported the association between various volatile organic compounds (VOCs) and phthalate metabolites with MetS. This cross-sectional study of a representative sample of adult South Koreans aimed to evaluate the relationship between heavy metals, VOC metabolites, phthalate metabolites, bisphenol A and MetS after adjusting for demographic variables. Data from the Korean National Environmental Health Survey II (2012–2014) (n = 5251) were used in the analysis. Multiple logistic regression analysis was performed for MetS with log-transformed hazardous material quartiles after covariate adjustment. Urine muconic acid (MuA) and mono- (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) levels were significantly associated with MetS after adjusting for confounders (odds ratio: 1.34 and 1.39, respectively). Urine MuA and MEHHP levels were significantly associated with MetS. Because of the rarity of this study, which investigated the relationship between each VOC and phthalate metabolite with MetS and the strict definition of all indirect measures of MetS components, further research is needed.

show abstract

CTBP1 and metabolic syndrome induce an mRNA and miRNA expression profile critical for breast cancer progression and metastasis

Cited by 22 publications

References 35 publications

CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice

CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice

A Circulating miRNA Signature for Stratification of Breast Lesions among Women with Abnormal Screening Mammograms

Association between Heavy Metals, Bisphenol A, Volatile Organic Compounds and Phthalates and Metabolic Syndrome

Contact Info

Product

Resources

About