CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway

Abstract

Objective The conditioned medium of calcined tooth powder (CTP‐CM) is a type of biomimetic mineralized material and well contributing to bone healing and bone formation in vivo. However, little is known about the effect of CTP‐CM on human periodontal ligament stem cells (hPDLSCs) as well as the underlying mechanisms. Methods ALP activity assay was conducted to select the concentration with the highest ALP level, which was used for the following experiments. Cell proliferation was measured by cell counting kit‐8 assay and flow cytometry analysis. Expression levels of osteogenic markers in CTP‐CM‐induced hPDLSCs were evaluated with real‐time quantitative reverse transcription polymerase chain reaction (qRT‐PCR), immunofluorescence staining, and Western blot. Mineralization of CTP‐CM‐induced hPDLSCs was evaluated by alizarin red staining. Furthermore, the involvement of NF‐κB pathway was examined by immunofluorescence staining and Western blot. Results 20 μg/ml was selected for the further experiments. Functional studies demonstrated that CTP‐CM exerted almost no influence on the proliferation of hPDLSCs and CTP‐CM increased the osteogenic differentiation of hPDLSCs. Mechanistically, CTP‐CM leads to activation of NF‐κB signaling pathway. When treated with BMS345541, the osteogenic differentiation of CTP‐CM‐treated hPDLSCs was significantly attenuated. Conclusion CTP‐CM can promote the osteogenic differentiation of hPDLSCs via activating NF‐κB pathway.

Extracellular vesicles from the inflammatory microenvironment regulate the osteogenic and odontogenic differentiation of periodontal ligament stem cells by miR-758-5p/LMBR1/BMP2/4 axis

“…PDLSCs ate more iDPSC-EV than DPSC-EV, indicating increased mineralization potential, consistent with the prior finding that EVs from MSCs favor PDLSCs for regeneration [41,42]. We chose to observe the third and seventh days of inducement to illustrate a dynamic detin and bone formation process [33,34].…”

“…The groups were marked as D0, D3 with DPSC-EV, D3 with iDPSC-EV, D7 with DPSC-EV, and D7 with iDPSC-EV. The evaluation was done by time and intergroup contrast at the same time point [33,34].…”

Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression

“…Our findings indicated that butyrate-a major subgingival bacterial metabolite-exhibited an important inhibitory effect on PDLSC osteogenic differentiation and periodontal tissue regeneration. It has been confirmed that many signalling pathways, such as the Smad, MAPK, and NF-κB pathways, are related to MSC osteogenic differentiation [31][32][33]. Here, we only explored the effect of NaB on the Smad pathway.…”

PD-1 Suppresses the Osteogenic and Odontogenic Differentiation of Stem Cells from Dental Apical Papilla via Targeting SHP2/NF-κB Axis

“…NF-κB signaling pathway is an important regulator of cellular gene transcription and can regulate differentiation [55]. When cells are induced by in ammatory complement, oxidative stress and other factors, leading to NF-κB pathway activation [56]. The activation of NF-κB pathway is characterized by the phosphorylation of P65 and IκBα in the cytoplasm, the translocation of P65 into the nucleus [57].…”