Cu(II): a “signaling molecule” of the mangrove endophyte Fusarium oxysporum ZZF51?

Pan, Jia-Hui; Lin, Yongcheng; Tan, Ni; Gu, Yu‐Cheng

doi:10.1007/s10534-010-9350-0

Cited by 9 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of FA to chelate metal ions, such as iron or copper, has been known for a long time (Lakshminarayanan and Subramanian, 1955;Malini, 1966;Pan et al, 2010;Tamari and Kaji, 1952). In a recent study, FA has been shown to chelate different metal ions, including Fe 21 , Fe 31 , Cu 21 , Mn 21 and Zn 21 (Ruiz et al, 2015;Yin et al, 2015).…”

Section: Effect Of Ph and Nutrients On Fub1 Transcript Levels And Fa mentioning

confidence: 99%

See 1 more Smart Citation

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts

López‐Díaz

Rahjoo

Sulyok

et al. 2017

Molecular Plant Pathology

114

View full text Add to dashboard Cite

Fusaric acid (FA) is amongst the oldest identified secondary metabolites produced by Fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of FA and its function in fungal pathogenicity remain unknown. Here, we investigated the role of FA in Fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. Targeted deletion of fub1, encoding a predicted orthologue of the polyketide synthase involved in FA biosynthesis in F. verticillioides and F. fujikuroi, abolished the production of FA and its derivatives in F. oxysporum. We further showed that the expression of fub1 was positively controlled by the master regulator of secondary metabolism LaeA and the alkaline pH regulator PacC through the modulation of chromatin accessibility at the fub1 locus. FA exhibited strong phytotoxicity on tomato plants, which was rescued by the exogenous supply of copper, iron or zinc, suggesting a possible function of FA as a chelating agent of these metal ions. Importantly, the severity of vascular wilt symptoms on tomato plants and the mortality of immunosuppressed mice were significantly reduced in fub1Δ mutants and fully restored in the complemented strains. Collectively, these results provide new insights into the regulation and mode of action of FA, as well as on the function of this phytotoxin during the infection process of F. oxysporum.

show abstract

Section: Effect Of Ph and Nutrients On Fub1 Transcript Levels And Fa mentioning

confidence: 99%

“…FA has long been known for its ability to chelate metal ions (Lakshminarayanan and Subramanian, 1955;Malini, 1966;Pan et al, 2010;Tamari and Kaji, 1952). However, the regulation of FA biosynthesis by metals has not been studied so far.…”

Section: Chromatin-mediated Regulation Of Fa Productionmentioning

confidence: 99%

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts

López‐Díaz

Rahjoo

Sulyok

et al. 2017

Molecular Plant Pathology

114

View full text Add to dashboard Cite

show abstract

“…It has been reported that copper-fusaric acid complexes are biologically relevant as they upregulate fusaric acid production by Fusarium sp (Pan et al 2010). Given that copper chelators are known to induce an undulated notochord phenotype, we infer that the binding of copper by fusaric acid induces this phenotype despite weak affinity measured in vitro (Tilton et al 2006).…”

Section: Resultsmentioning

confidence: 70%

Fusaric acid induces a notochord malformation in zebrafish via copper chelation

et al. 2015

View full text Add to dashboard Cite

Over a thousand extracts were tested for phenotypic effects in developing zebrafish embryos to identify bioactive molecules produced by endophytic fungi. One extract isolated from Fusarium sp., a widely distributed fungal genus found in soil and often associated with plants, induced an undulated notochord in developing zebrafish embryos. The active compound was isolated and identified as fusaric acid. Previous literature has shown this phenotype to be associated with copper chelation from the active site of lysyl oxidase, but the ability of fusaric acid to bind copper ions has not been well described. Isothermal titration calorimetry revealed that fusaric acid is a modest copper chelator with a binding constant of 4.4 × 10(5) M(-1). These results shed light on the toxicity of fusaric acid and the potential teratogenic effects of consuming plants infected with Fusarium sp.

show abstract

“…and leaf spot pathogens (such as different species of Alternaria) were also recovered from apparently healthy prickly acacia plants. Many of these fungi were also previously reported as being endophytes (Teleha & Miller 2007;Pan et al 2010;Porras-Alfaro et al 2014;Swett & Gordon 2015).…”

Section: Major Findingsmentioning

confidence: 73%

Investigation of the fungi associated with dieback of prickly acacia (Vachellia nilotica subsp. indica) in Northern Australia

Haque¹

View full text Add to dashboard Cite

Prickly acacia (Vachellia nilotica subsp. indica), one of the most harmful weeds of the Australian rangelands, has been occasionally observed displaying natural dieback symptoms since 1970's.More recently in 2010, a prominent widespread dieback event was observed among the plants growing around Richmond and Julia Creek in north-western Queensland. Affected plants were found with disease symptoms such as; ashy internal staining, defoliation, blackening of shoot tips through to widespread plant mortality. It was hypothesized that a pathogenic fungus/i could be implicated with this phenomenon and a potential for biological control of this invasive species might ensue.Fungi isolated from dieback-affected and healthy stands of prickly acacia were putatively identified by partial sequencing of the Internal Transcribed Spacer (ITS) region of genomic DNA.Botryosphaeriaceae was the best represented family of fungi associated with dieback. Among the Botryosphaeriaceae fungi, Cophinforma was found to be the most prevalent genus with 60% of the total isolates identified as Cophinforma spp. following BLAST searches. Cophinforma was also isolated from the healthy plants growing in the trial site at Richmond. Natural dieback on prickly acacia was previously observed in the surrounding areas. Phylogenetic analysis of the ITS sequences revealed the potential existence of new species of Cophinforma in Australia. Apart from Cophinforma sp., a range of other Botryosphaeriaceae and non-Botryosphaeriaceae fungi were isolated from both dieback-affected and healthy prickly acacia plants growing at different locations.Based on a seedling pathogenicity screening, a sub-set of 40 potentially pathogenic isolates across the range of species were selected to challenge the juvenile plants following a stem inoculation technique under glasshouse conditions. In the glasshouse trials, the test-isolates of Cophinforma sp.(isolated from both dieback-affected and healthy prickly acacia) and an isolate of Lasiodiplodia pseudotheobromae strain NT039 (isolated from dieback-affected Parkinsonia aculeata) consistently caused significant stem lesions, gummosis and leaf mortality. Cophinforma sp., Fusarium acuminatum, Neofusicoccum parvum and Tiarosporella graminis isolated from healthy prickly acacia also caused similar symptoms in glasshouse inoculation trials. The remaining species tested in the glasshouse trials were mostly non-pathogenic to the juvenile plants.In another study, given the observation that several fungi are often isolated from a single piece of dieback-affected stem, it was investigated whether typical dieback symptoms in prickly acacia could be replicated with combinations of different fungi. Therefore, the effect of inoculating with iii two isolates of Cophinforma sp. and one isolate of L. pseudotheobromae singly and in combination under glasshouse and field conditions was tested. In the glasshouse trial, significant stem lesions were observed with each of the test-isolates and their combinations. However, there was no signifi...

show abstract

Cu(II): a “signaling molecule” of the mangrove endophyte Fusarium oxysporum ZZF51?

Cited by 9 publications

References 21 publications

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts

Fusaric acid induces a notochord malformation in zebrafish via copper chelation

Investigation of the fungi associated with dieback of prickly acacia (Vachellia nilotica subsp. indica) in Northern Australia

Contact Info

Product

Resources

About