Cu/Zn- and Mn-Superoxide Dismutase Distribution and Concentration in Adrenal Tumors

Activity, thermostability and isozymes of superoxide dismutase in 17 edible mushrooms

“…The dyeing density of SOD band increased with increasing concentration of SOD, there was a linear relationship within the range of 0.5-500 ng SOD (Fig. 5A, 7-15), which meant that the sensitivity of SOD detection on PGGE gel reached the level of an enzyme-linked immunosorbent (Inagaki et al, 1989;Michalski, 1996;Iwase et al, 2006;Kawaguchi et al, 1990) and chemiluminescence assay (Corbisier et al, 1987).…”

Species specific isotope dilution versus internal standardization strategies for the determination of Cu, Zn-superoxide dismutase in red blood cells

“…13 Also, a decrease in SOD levels (in e.g., malignant lymphoma patients, chronic alcoholism or medullar tumours where the concentration decreases from 353 AE 62 ng g À1 in normal tissues to 123 AE 13 ng mL À1 ) can be observed. 14,15 In addition to biological methods, some other alternatives have been recently proposed with the final aim of Cu, Zn-SOD absolute quantification by inductively coupled plasma mass spectrometric (ICP-MS) consisting of determining the Cu bound to the biomolecule and calculating the protein content from the known metal:protein stoichiometry. Some of these strategies are based on the appropriate gel electrophoretic separation of the protein followed by the elemental detection of the Cu and Zn on the ablated protein spots by ICP-MS. 16 In order to compensate for potential losses during the analytical procedure, Feldmann et al have used the synthesized isotopically labelled 65 Cu, 68 Zn-SOD that permits also the absolute quantification of the protein.…”

Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis

“…Three distinct isoforms of SOD, i.e., copper-zinc superoxide dismutase (Cu/Zn-SOD), manganese SOD (Mn-SOD), and extracellular SOD, have been identiWed in mammals (Zelko et al 2002), of which Cu/Zn-SOD and Mn-SOD are the major intracellular antioxidant enzymes and have generated great interest as potential targets of human carcinogenesis (Iwase et al 2006;Morandini et al 1999). A paradigm for SOD function in cancer is based on the potency of a cellular defense mechanism against ROS, including true free radicals, the superoxide anion and the hydroxyl radical, and non-radical compounds, such as hydrogen peroxide (H 2 O 2 ) (Hileman et al 2001;Kinnula and Crapo 2004).…”