Cu/Zn-superoxide dismutase from the fungal strain Humicola lutea 103 improves ram spermatozoa functions in vitro

Stefanov, R.; Angelova, Maria; Stefanova, Tsvetanka; Subev, M.; Dolashka, Pavlina; Voelter, Wolfgang; Zachariev, Z.

doi:10.1111/j.1439-0272.2004.tb02977.x

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…Furthermore, addition of antioxidants analogues of superoxide dismutase in alpaca semen extender prevents loss of motility and DNA fragmentation (Santiani et al, 2013). In this way, it is probably that high levels of viable spermatozoa producing superoxide anion and hydrogen peroxide could be related to low sperm motility, as reported in ram semen (Peris, Bilodeau, Dufour, & Bailey, 2007;Stefanov et al, 2004).…”

Section: Discussionmentioning

confidence: 72%

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Evangelista‐Vargas

Santiani

2017

Reprod Domestic Animals

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The objective of this study was to detect changes in intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) production and lipid peroxidation during cryopreservation of alpaca spermatozoa. Twelve alpaca semen samples were conventionally cryopreserved. Intracellular superoxide anion and hydrogen peroxide were evaluated by fluorescence microscopy using dihydroethidium (DHE)/YO-PRO-1 and dichlorofluorescein diacetate (H2DCFDA)/propidium iodide (PI), respectively. Evaluations were performed during cooling curve at (1) 25°C, (2) 15°C, (3) 5°C/0 min, (4) 5°C/15 min, (5) 5°C/30 min and (6) after freezing/thawing. Evaluation of lipid peroxidation by measuring malondialdehyde (MDA) was performed at 25°C, 5°C/30 min and after thawing. Maximum percentages of total spermatozoa producing superoxide anion and hydrogen peroxide were found at 5°C/30 min (62.8 ± 6.3% and 30.5 ± 5.6%, respectively), and these results were higher (p < .05) than initial (25°C: 10.8 ± 3.8% and 6.8 ± 0.7%, respectively) and after thawing (29.8 ± 9.5% and 7.5 ± 1.8%, respectively) values. However, considering only viable spermatozoa, production of superoxide anion and hydrogen peroxide during overall stabilization at 5°C (>76% and >91%, respectively) and after thawing (74.9 ± 5.0% and 78.9 ± 2.2%, respectively) was higher (p < .05) than initial values at 25°C (38.7 ± 3.1% and 53.6 ± 2.0%, respectively). Lipid peroxidation at 25°C, 5°C/30 min, and post-thawing were 346.5 ± 99.8, 401.1 ± 64.8 and 527.7 ± 142.8 ng/ml MDA, respectively. These results showed that high percentage of viable alpaca spermatozoa produces intracellular reactive species oxygen (ROS) during the cryopreservation process of alpaca semen.

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Section: Discussionmentioning

confidence: 72%

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Evangelista‐Vargas

Santiani

2017

Reprod Domestic Animals

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“…Thus, the addition of SOD together with copper and zinc has been tested in cryopreservation extenders. Three different concentrations of SOD (30, 60 and 120 U ⁄ ml) were added in the incubation of ram semen for 5 h (39°C) and found that the higher concentrations of SOD were more effective than the lowest concentration (30 U ⁄ ml) (Stefanov et al 2004). The authors state that the exogenous administration of SOD suppressed the formation of H 2 O 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Superoxide dismutase (SOD) has enzymatic action and plays an important antioxidant role by catalysing the dismutation of the superoxide anion radical in H 2 O 2 and O 2 in the presence of the H proton (Ferreira and Matsubara 1997). Cryopreservation processes are associated to a reduction in organic levels of GSH (Gardo´n et al 2003) and SOD (Stefanov et al 2004), likely due to the dilution of the semen, thereby favouring the production of ROS and consequently leading to damage to sperm cells (Guerra et al 2004).…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione

Silva

Soares

Batista

et al. 2011

Reprod Domestic Animals

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The aim of the present study was to evaluate the in vitro and in vivo effect of the addition of superoxide dismutase (SOD) and reduced glutathione (GSH) to ram semen freezing extender. Significant differences (p < 0.05) were detected between groups regarding total motility (TM), straightness (STR) and wobble (WOB), for which the GSH 7 mM group had lesser TM and better STR than the other groups and the GSH 5 and 7 mM groups had higher wobble values than the control, SOD 25 and 100 U/ml groups. The ultrastructural analysis revealed that the acrosome was better preserved after freezing in the SOD 100 U/ml and GSH 2 and 5 mM (p < 0.05) groups than the other groups, whereas mitochondria in both the control group and the 7 mM GSH group suffered the greatest damage. The plasma membrane remained preserved after freezing, regardless of the group. For in vivo fertilization, the SOD group achieved better results than the GSH group (p > 0.05). It can therefore be concluded that the addition of SOD 100 U/ml and GSH 2 and 5 mM preserves the acrosome integrity of frozen ram spermatozoa, while the addition of SOD 100 U/ml to Tris egg-yolk extender offers protection to the membranes of sperm cells after thawing.

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“…The group of enzymes referred to this catalyzes oxide-reduction reaction, contributing to the maintenance of intracellular balance and the energy is released for the cell metabolism [6]. The enzyme system NADH-tetrazolium reductase can be attached to them and used as marker for the functional activity of sperm mitochondrion [12,14]. The aim of this study was to analyze the activity of NADH-tetrazolium reductase enzyme system in ram semen during liquid and cryopreservation in relation to their viability.…”

Section: Introductionmentioning

confidence: 99%

Activity of Nadh-Tetrazolium Reductase in Ram Semen During Liquid and Cryopreservation

Stefanov¹

2010

JCEA

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The investigations for the determination of NADH-tetrazolium reductase enzyme system activity in ram semen during liquid and cryopreservation in relation with their viability, were carried out. The semen samples with lower motility of spermatozoa after collection, possess lower values of mean cytochemical coefficient for NADH-tetrazolium reductase activity ( р<0.5, р<0.5) after liquid and cryopreservation.KEY WORDS: spermatozoa, NADH-tetrazolium reductase, motility, sperm metabolism РЕЗЮМЕПроведени са изследвания за определяне активността на НАДН-тетразолий редуктазна ензимна система в сперматозоиди от коч при краткротрайно съхранение и криоконсервация, във връзка с виталитета им. При сперма с понижен мотилитет на сперматозоидите при получаване, след краткротрайно съхранение и след криоконсервация, са установени достоверно по-ниски стойности на СЦК за НАДН-ТР-активност ( р<0.5, р<0.5). Rossen STEFANOV ПОДРОБНО РЕЗЮМЕПроведени са изследвания за определяне активността на НАДН-тетразолий редуктазна ензимна система в сперматозоиди от коч при краткротрайно съхранение и криоконсервация, във връзка с виталитета им. В експериментите включихме 14 еякулата, получени по метода на изкуствена вагина от три разплодника изравнени по възраст (2 години), поставени при еднакви условия на хранене, гледане и полово използване, съобразени с нормативните изисквания. Еякулатите разделяхме на четири части, разредени в съотношение 1+3, като при първите ( А и В) използвахме среда за съхраняване при 4°С , а при вторите( С и D) -среда на Нагазе-Нива за криоконсервация. В пробите от групи А иС включихме еякулати с подвижност 65-70%, а в групите В и D такива с подвижност над 70% Установено е, че при пресни еякулати от коч с понижен витатитет (под 70%), както и след криоконсервацията им, стойности по отношение на мотилитета на сперматозоиди след инкубация при 39°С за 300 мин. са достоверно по-ниски (р<0.01, р<0.01). При сперма с понижен мотилитет на сперматозоидите при получаване (под 70%), след краткротрайно съхранение и след криоконсервация, са установени достоверно по-ниски стойности на СЦК за НАДН-ТР-активност ( р<0.5, р<0.5). Стойностите на СЦК за НАДН-ТР-активност в сперматозоидите могат да се използват като критерий за динамика на метаболитни процеси и виталитета на сперматозоидите от коч.

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Cu/Zn-superoxide dismutase from the fungal strain Humicola lutea 103 improves ram spermatozoa functions in vitro

Cited by 6 publications

References 27 publications

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa

In Vitro and In Vivo Evaluation of Ram Sperm Frozen in Tris Egg‐yolk and Supplemented with Superoxide Dismutase and Reduced Glutathione

Activity of Nadh-Tetrazolium Reductase in Ram Semen During Liquid and Cryopreservation

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