CUBIC-plus: An optimized method for rapid tissue clearing and decolorization

Ren, Zhengyu; Wu, Yuzheng; Wang, Zhenning; Hu, Yang; Lu, Jie; Liu, Jinhui; Chen, Yalan; Yao, Maojin

doi:10.1016/j.bbrc.2021.06.075

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…(2021). 19 We shake 3 days for the oviduct and 6 days for the mammary gland. Wash the organs with PBS to wash off CUBIC reagent-1, put them into fresh PBS, shake gently (100 rpm) at room temperature for 30 min.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

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Protocol for integrating tissue clearing and light-sheet imaging to analyze cancer initiation in mosaic analysis with double markers mouse models

Zeng,

Ball,

Zhou

et al. 2024

STAR Protocols

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…The suggested time provided by Ren et al. (2021) 19 is based on organs obtained from 8-week-old female mice. If tissues are harvested from older mice, increasing the duration of shaking with CUBIC reagent-1 by 1.5–2 times is suggested to improve transparency.…”

Section: Troubleshootingmentioning

confidence: 99%

Protocol for integrating tissue clearing and light-sheet imaging to analyze cancer initiation in mosaic analysis with double markers mouse models

Zeng,

Ball,

Zhou

et al. 2024

STAR Protocols

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“…Similarly, in HeLa subcutaneous xenografts, lectin was used to map the distribution of nanoparticles in tumors cleared with CUBIC (56). Dextran can also be used to label blood vasculature, as shown in A549 lung tumors cleared with CUBIC-plus (57). Dextran permeates through fenestrated vessels and can hence be used to evaluate the integrity and permeability of the vasculature, as demonstrated in GBM xenografts cleared in ECi (38).…”

Section: Visualizing the Tumor Vasculature With Tissue Clearingmentioning

confidence: 99%

Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time

Almagro

Messal

2023

Front. Immunol.

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Volume imaging visualizes the three-dimensional (3D) complexity of tumors to unravel the dynamic crosstalk between cancer cells and the heterogeneous landscape of the tumor microenvironment (TME). Tissue clearing and intravital microscopy (IVM) constitute rapidly progressing technologies to study the architectural context of such interactions. Tissue clearing enables high-resolution imaging of large samples, allowing for the characterization of entire tumors and even organs and organisms with tumors. With IVM, the dynamic engagement between cancer cells and the TME can be visualized in 3D over time, allowing for acquisition of 4D data. Together, tissue clearing and IVM have been critical in the examination of cancer-TME interactions and have drastically advanced our knowledge in fundamental cancer research and clinical oncology. This review provides an overview of the current technical repertoire of fluorescence volume imaging technologies to study cancer and the TME, and discusses how their recent applications have been utilized to advance our fundamental understanding of tumor architecture, stromal and immune infiltration, vascularization and innervation, and to explore avenues for immunotherapy and optimized chemotherapy delivery.

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“…In recent years, tissue clearing technologies have developed rapidly. Therefore, thick tissues can be cleared and become transparent; thus, more complete and abundant information, including the vascular network, could be obtained [1,[11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…For transgenic animals, the acquisition and maintenance costs are high [20,24,34]. In contrast, probe labeling is gradually favored by the academic community because of its simple operation process, low cost and good labeling effect [14,20,35]. However, there are many kinds of probes and different ways to label, all of which may deliver different labeling effects.…”

Section: Introductionmentioning

confidence: 99%

A simple and effective vascular network labeling method for transparent tissues of mice

Bai

Dai

et al. 2023

Journal of Biophotonics

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Vascular network labeling in transparent tissues provides more complete information on blood vessels. To achieve a fast and efficient method for vascular network labeling in transparent tissues, we compared various vascular labeling methods under different tissue clearing protocols. FITC-Dextran labeling and CUBIC cleaning treatment were found to be the best options for vascular network labeling in cleared mouse tissues. Satisfactory labeling of vascular networks in various organs can be achieved by selecting FITC-Dextran with different molecular weights and different administration methods.

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CUBIC-plus: An optimized method for rapid tissue clearing and decolorization

Cited by 10 publications

References 24 publications

Protocol for integrating tissue clearing and light-sheet imaging to analyze cancer initiation in mosaic analysis with double markers mouse models

Protocol for integrating tissue clearing and light-sheet imaging to analyze cancer initiation in mosaic analysis with double markers mouse models

Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time

A simple and effective vascular network labeling method for transparent tissues of mice

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