Cudratricusxanthone A Inhibits Lipid Accumulation and Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes

Kwon, Hyo-Shin; Jeong, Gil‐Saeng; Jang, Byeong‐Churl

doi:10.3390/ijms22020505

Cited by 2 publications

(4 citation statements)

References 57 publications

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“…It is further documented that TNF-α is a strong inducer of COX-2 in various cells, including preadipocytes (15,16). Indeed, the authors of the present study as well as other groups have demonstrated that the short-term exposure of TNF-α leads to induction of COX-2 expression in 3T3-L1 preadipocytes (16)(17)(18). Thus, inhibition of the TNF-α-induced COX-2 expression in preadipocytes is considered as a possible target to manage obesity inflammation.…”

Section: Introductionsupporting

confidence: 70%

“…A wealth of information illustrates that preadipocytes, the predominant cell types present in the AT, are able to express and secrete numerous pro-inflammatory cytokines [including TNF-α, interleukin (IL)-1β and IL-6] and enzymes (COX-2) (49-51), which can confer and aggravate obese inflammation, via the autocrine, paracrine and endocrine ways. As aforementioned, the authors and other groups have previously demonstrated that TNF-α is an inducer of COX-2 in not only 3T3-L1 preadipocytes but also in mature or differentiated adipocytes (15)(16)(17)(18)(19). To date, the regulation of pro-inflammatory cytokine-induced expression of COX-2 by LNX in 3T3-L1 preadipocytes remains unknown.…”

Section: Discussionmentioning

confidence: 89%

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Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes

Perumal,

Do,

Choi

et al. 2023

Biomed Rep

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Nutmeg is the seed derived from Myristica fragrans . Nutmeg seeds contain alkylbenzene derivatives such as myristicin, which are toxic to the human organism, and lignan compounds such as nectandrin B, which possess anti-aging and anti-diabetic properties. However, the anti-adipogenic, prolipolytic and anti-inflammatory effects of lignan-enriched nutmeg extract (LNX) on preadipocytes remain unclear. In the present study, the effects of LNX on lipid accumulation, glycerol release and inflammatory cyclooxygenase-2 (COX-2) expression in differentiated 3T3-L1 preadipocytes were investigated. Oil red O staining demonstrated that treatment with LNX resulted in a concentration-dependent reduction in lipid accumulation in differentiating 3T3-L1 preadipocytes without affecting cell growth. Mechanistically, LNX treatment at 6 µg/ml led to a reduction in phosphorylation levels of signal transducer and activator of transcription 3 (STAT3), whereas it did not influence the peroxisome proliferator-activated receptor gamma (PPAR-γ) and CCAAT enhancer binding protein alpha (C/EBP-α) expression levels during 3T3-L1 preadipocyte differentiation. In addition, LNX treatment at 6 µg/ml led to a decrease in fatty acid synthase (FAS) expression levels on day (D) 2, but not D5 and D8, during preadipocyte differentiation. Treatment with LNX at 6 µg/ml did not affect the expression levels of perilipin A during preadipocyte differentiation. In differentiated 3T3-L1 adipocytes, LNX treatment at 6 µg/ml did not stimulate glycerol release and hormone-sensitive lipase phosphorylation, which are known lipolysis hallmarks. Furthermore, LNX treatment at the doses tested had no effect on tumor necrosis factor alpha-induced COX-2 expression in 3T3-L1 preadipocytes. Collectively, these results demonstrated that LNX has an anti-adipogenic effect on differentiating 3T3-L1 preadipocytes, which is mediated by the downregulation of STAT3 phosphorylation and FAS expression.

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Section: Introductionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 89%

Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes

Perumal,

Do,

Choi

et al. 2023

Biomed Rep

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“…Of interest, it has been previously shown that TNF-α is a strong inducer of iNOS in many types of cells, including (pre) adipocytes [24]. Supporting this, we also have recently demonstrated the ability of TNF-α to induce iNOS expression in 3T3-L1 (pre)adipocytes [25]. However, at present, the inducer(s) and its regulation of iNOS expression in (pre)adipocytes remain unclear.…”

Section: Introductionsupporting

confidence: 62%

“…Aforementioned, we and others have previously shown that TNF-α, another pro-inflammatory cytokine, up-regulates the expression of iNOS in (pre)adipocytes [24,25]. Given that (pre)adipocytes express and secrete not only TNF-α but also other pro-inflammatory cytokines, and they are exposed to many endogenic and exogenic stimuli, we herein have investigated the effects of endogenic (IL-1β, IL-10, IL-12, IFN-γ) and exogenic (LPS) stimuli on the expression of iNOS in 3T3-L1 preadipocytes.…”

Section: Discussionmentioning

confidence: 95%

Interleukin 1β Up-Regulates mRNA Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes: Role of JAKs/STATs, PKCs, and Src

2021

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Recent evidence suggests obesity as a low or systemic chronic inflammation. (Pre) adipocytes in the adipose tissue (AT) express and secrete a variety of cytokines and adipokines. Inducible nitric oxide synthase (iNOS) is an inflammatory enzyme involved in the production of NO. Until now, the inducer(s) of iNOS expression in (pre)adipocytes remains unclear. In this study, we investigated the effects of proinflammatory cytokines [interleukin-1β (IL-1β), IL-10, IL-12, interferon-γ (IFN-γ)], adipokines [retinol-binding protein 4 (RBP4), adiponectin, leptin, and resistin], and lipopolysaccharide (LPS), a bacterial cell wall component, on the expression of iNOS in 3T3-L1 preadipocytes. Notably, treatment with IL-1β at 20 ng/mL for 4 h markedly increased iNOS mRNA expression in 3T3-L1 preadipocytes, but that with IL-10 (10 ng/mL), IL-12 (5 ng/mL), IFN-γ (10 ng/mL), RBP4 (5 ug/mL), adiponectin (100 ng/mL), leptin (100 ng/mL), and resistin (100 ng/mL), and LPS (1 ug/mL) for 4 h had little or no effect on it. Results of dose-response and time-course experiments confirmed the ability of IL-1β at 20 ng/mL for 4 h to maximally induce iNOS mRNA expression in 3T3-L1 preadipocytes. Importantly, pharmacological inhibition studies demonstrated that treatment with AG490 [an inhibitor of Janus-activated kinases (JAKs) and signal transducer and activator of transcription proteins (STATs)], GO6976 (an inhibitor of PKCs), or PP1 (an Src kinase inhibitor) suppressed IL-1βinduced iNOS mRNA expression in 3T3-L1 preadipocytes, pointing out the involvement of JAKs/STATs, PKCs, and Src in the process. This work advocates that IL-1β is a major and strong inducer of iNOS expression in 3T3-L1 preadipocytes.

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Cudratricusxanthone A Inhibits Lipid Accumulation and Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes

Cited by 2 publications

References 57 publications

Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes

Anti‑adipogenic effect and underlying mechanism of lignan‑enriched nutmeg extract on 3T3‑L1 preadipocytes

Interleukin 1β Up-Regulates mRNA Expression of Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes: Role of JAKs/STATs, PKCs, and Src

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