CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

Zhu, Ling; Bu, Qingyun; Xu, Xiaosa; Paik, Inyup; Huang, Xi; Hoecker, Ute; Deng, Xing Wang; Huq, Enamul

doi:10.1038/ncomms8245

Cited by 113 publications

(146 citation statements)

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“…In this process, phy interaction is necessary for light-induced phosphorylation, polyubiquitylation and subsequent degradation (Leivar and Quail, 2011). Both CUL3-LRB and CUL4-COP1-SPA complexes have been shown to function as E3 ubiquitin ligases for the light-induced degradation of PIF3 and PIF1, respectively (Ni et al, 2014;Xu et al, 2015;Zhu et al, 2015;Zhu and Huq, 2014). In addition, …”

Section: Discussionmentioning

confidence: 99%

“…Previously, both PIF1 and PIF3 have been shown to be unstable in cop1-4 plants in the dark (Bauer et al, 2004;Zhu et al, 2015). We have examined the PIF5 level in cop1-4 seedlings grown in the dark.…”

Section: Pifs Are Degraded In the Dark Via The 26s Proteasome Pathwaymentioning

confidence: 96%

“…S1) (Bauer et al, 2004;Zhu et al, 2015). However, in imbibed seeds, the PIF1 level is much higher in cop1-4 and the quadruple mutant spaq (spa1 spa2 spa3 spa4) compared with wild type .…”

Section: Hfr1 Promotes the Degradation Of Pif1 And Pif5mentioning

confidence: 99%

“…CK2 (casein kinase II) and BIN2 have been shown to phosphorylate PIF1 and PIF4, respectively, in a light-independent manner (Bernardo-García et al, 2014;Bu et al, 2011b). In addition, both CUL3 and CUL4-based E3 ligase complexes mediate the light-induced ubiquitylation of PIF3 and PIF1, respectively, during dark-to-light transition (Ni et al, 2014;Zhu et al, 2015). HEMERA has also been shown to induce degradation of PIFs under de-etiolated conditions possibly in a transcription-coupled manner (Qiu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis

Kathare

Pham

et al. 2017

Development

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The phytochrome-mediated regulation of photomorphogenesis under red and far-red light conditions involves both positively and negatively acting factors. The positively acting factors (e.g. HY5/HFR1/LAF1 and others) are degraded in the dark to prevent photomorphogenesis. By contrast, the negatively acting factors (e.g. phytochromeinteracting factors or PIFs) are degraded in response to light to promote photomorphogenesis. Here, we show that the negatively acting factor PIF1 is also degraded in the dark by direct heterodimerization with the positively acting factor HFR1. Conversely, PIF1 also promotes the degradation of HFR1 in darkness. PIF1 enhances the poly-ubiquitylation of HFR1 by COP1 in vivo and in vitro. In addition, the reciprocal co-degradation of PIF1 and HFR1 is dependent on the 26S proteasome pathway in vivo. Genetic evidence shows that the hfr1 mutant partially suppresses the constitutive photomorphogenic phenotypes of cop1-6 pif1 and of the quadruple mutant pifq both in the dark and in far-red light conditions. Taken together, these data uncover a co-degradation mechanism between PIFs and HFR1 that underlies photomorphogenic development in Arabidopsis thaliana.

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Section: Discussionmentioning

confidence: 99%

Section: Pifs Are Degraded In the Dark Via The 26s Proteasome Pathwaymentioning

confidence: 96%

“…S1) (Bauer et al, 2004;Zhu et al, 2015). However, in imbibed seeds, the PIF1 level is much higher in cop1-4 and the quadruple mutant spaq (spa1 spa2 spa3 spa4) compared with wild type .…”

Section: Hfr1 Promotes the Degradation Of Pif1 And Pif5mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis

Kathare

Pham

et al. 2017

Development

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“…However, the degradation kinetics of native PIF1 under light is similar in both the wild type and hec1 hec2 double mutant. Reasoning that this might be due to extremely rapid degradation of native PIF1 Zhu et al, 2015), we used the HEC2 overexpression plants where both quantitative and regular RT-PCR assays showed increased level of HEC2 mRNA in two independent HEC2 overexpression lines (Supplemental Figure 4). Results show that the light-induced degradation of PIF1 is greatly reduced in HEC2 overexpression line under both dark and light conditions ( Figures 9A and 9B).…”

Section: Hec2 Reduces the Light-induced Degradation Of Pif1mentioning

confidence: 99%

A Negative Feedback Loop between PHYTOCHROME INTERACTING FACTORs and HECATE Proteins Fine-Tunes Photomorphogenesis in Arabidopsis

Zhu

Xin

et al. 2016

Plant Cell

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The phytochrome interacting factors (PIFs), a small group of basic helix-loop-helix transcription factors, repress photomorphogenesis both in the dark and light. Light signals perceived by the phytochrome family of photoreceptors induce rapid degradation of PIFs to promote photomorphogenesis. Here, we show that HECATE (HEC) proteins, another small group of HLH proteins, antagonistically regulate PIFs to promote photomorphogenesis. HEC1 and HEC2 heterodimerize with PIF family members. PIF1, HEC1, and HEC2 genes are spatially and temporally coexpressed, and HEC2 is localized in the nucleus. hec1, hec2, and hec3 single mutants and the hec1 hec2 double mutant showed hyposensitivity to light-induced seed germination and accumulation of chlorophyll and carotenoids, hallmark processes oppositely regulated by PIF1. HEC2 inhibits PIF1 target gene expression by directly heterodimerizing with PIF1 and preventing DNA binding and transcriptional activation activity of PIF1. Conversely, PIFs directly activate the expression of HEC1 and HEC2 in the dark, and light reduces the expression of these HECs possibly by degrading PIFs. HEC2 is partially degraded in the dark through the ubiquitin/26S-proteasome pathway and is stabilized by light. HEC2 overexpression also reduces the light-induced degradation of PIF1. Taken together, these data suggest that PIFs and HECs constitute a negative feedback loop to fine-tune photomorphogenesis in Arabidopsis thaliana.

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Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

Yoo

Han

Chen

2020

Annual Plant Reviews Online

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The biogenesis of photosynthetically active chloroplasts in flowering plants (angiosperms) is initiated by light through the red and far‐red photoreceptors, phytochromes, which activate photosynthesis‐associated genes encoded by both the nuclear and plastid genomes. Because photoactivated phytochromes localize to the nucleus but not the plastids, phytochromes ought to control plastid transcription through nucleus‐to‐plastid or anterograde signalling. However, the mechanism of anterograde signalling remains poorly understood. Recent advances in our understanding of phytochrome signalling and plastid transcription led to the discovery of the framework of an anterograde signalling pathway, which links light‐dependent regulation of nuclear repressors of chloroplast biogenesis to the control of the assembly and activity of the bacterial‐type plastidial RNA polymerase for transcribing plastid‐encoded photosynthesis genes. This article is aimed to gather and reanalyze the evidence in the literature relevant to the phytochrome control of chloroplast biogenesis to forge a model of anterograde signalling mechanisms for the regulation of plastid transcription by light.

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CUL4 forms an E3 ligase with COP1 and SPA to promote light-induced degradation of PIF1

Cited by 113 publications

References 50 publications

Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis

Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlies photomorphogenic development in Arabidopsis

A Negative Feedback Loop between PHYTOCHROME INTERACTING FACTORs and HECATE Proteins Fine-Tunes Photomorphogenesis in Arabidopsis

Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

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