Culex vishnui

Maquart, Pierre‐Olivier; Boyer, Sébastien

doi:10.1016/j.pt.2022.01.003

Cited by 4 publications

(3 citation statements)

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“…In Asia, for example, cryptic members of the Cx. vishnui species group confound tracking of Japanese encephalitis virus transmission (53). The morphological differences between the Culex species bitaeniorhynchus , tritaeniorhynchus , vishnui , and pseudovishnui are often elusive, the former three having been morphologically identified in our study but later revealed by COI barcoding to be another species.…”

Section: Discussionmentioning

confidence: 99%

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Decoding rRNA sequences for improved metagenomics of sylvatic mosquito species

Koh

Frangeul

Blanc

et al. 2022

Preprint

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RNAseq-based metagenomics on mosquitoes are frequently performed for surveillance or microbiome discovery studies to understand the disease ecology of arboviruses. A major hurdle to overcome in these studies is the depletion of abundant rRNA, which is commonly achieved using oligo-based protocols. The lack of complete reference rRNA sequences in sequence databases for most mosquito vectors makes such studies feasible for only a narrow range of species, biasing the knowledge on mosquitoes and their viruses. We performed total RNAseq and mitochondrial cytochrome c oxidase I gene sequencing on specimens of 29 mosquito species to compare the functionality of rRNA sequences as an RNA barcode for taxonomy and phylogeny. Using a score-based strategy to bioinformatically filter out contaminating rRNA reads, we successfully assembled full-length 28S and 18S rRNA sequences for all specimens. Phylogenetic reconstructions revealed a high degree of congruity between DNA and RNA barcodes. This assembly strategy presented here and the expansion of the rRNA reference library in public databases by 232 new complete 28S and 18S rRNA sequences provide new tools to improve surveillance and microbiome discovery for a more holistic understanding of arbovirus-transmission by mosquitoes in designing preemptive measures.

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Section: Discussionmentioning

confidence: 99%

“…vishnui , and Cx. pseudovishnui , which are important vectors of JEV (Maquart & Boyer, 2022). The former two were morphologically identified in our study but later revealed by COI barcoding to be a sister species.…”

Section: Discussionmentioning

confidence: 99%

Decoding rRNA sequences for improved metagenomics of sylvatic mosquito species

Koh

Frangeul

Blanc

et al. 2022

Preprint

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“…In Thailand, it is estimated that 15% (22/147) of hospitalised encephalitis cases were caused by JEV despite vaccine being introduced since 2000 (Olsen et al, 2010). JEV is maintained by an enzootic transmission cycle between aquatic birds and swine, with Culex species acting as main vectors (Maquart & Boyer, 2022). Humans can be affected but are considered to be dead‐end host for the virus cycle (Dash et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Phylogeny and spatial distribution of Japanese encephalitis virus vector species in Cambodia

Dusadeepong

Maquart

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et al. 2023

Medical Vet Entomology

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In Southeast Asia, despite the use of Japanese encephalitis vaccines and vaccination coverage, Japanese encephalitis (JE) transmission is still a major public health issue. The main vectors of this virus are mosquitoes from the genus Culex, which diversity and density are important in Southeast Asia. The main vector species of Japanese encephalitis virus (JEV) in Cambodia belong to the Vishnui subgroup. However, their morphological identification solely based on the adult stage remains challenging, making their segregation and detection difficult.In order to identify and describe the distribution of the three main JEV vector species in Cambodia, namely Culex vishnui, Cx. pseudovishnui and Cx. tritaeniorhynchus, mosquito samplings were carried out throughout the country in different environments. Phylogenetic analysis of the cytochrome c oxidase subunit I (coI) gene using maximum‐likelihood tree with ultrafast bootstrap and phylogeographic analysis were performed. The three main Culex species are phylogenetically separated, and represent two distinct clades, one with Cx. tritaeniorhynchus and the second with Cx. vishnui and Cx. pseudovishnui, the latter appearing as a subgroup of Cx. vishnui. The phylogeographic analysis shows a distribution of the Vishnui subgroup on the entire Cambodian territory with an overlapped distribution areas leading to a sympatric distribution of these species. The three JEV vector species are geographically well‐defined with a strong presence of Cx. pseudovishnui in the forest. Combined with the presence of Cx. tritaeniorhynchus and Cx. vishnui in rural, peri‐urban, and urban areas, the presence of JEV‐competent vectors is widespread in Cambodia.

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