Cultivable Anaerobic Microbiota of Infected Root Canals

Sato, Takuichi; Yamaki, Kouya; Ishida, Naoko; Hara, Kazuhiro; Takeuchi, Yasuhisa; Shoji, Megumi; Sato, Emika; Matsuyama, Junko; Shimauchi, Hidetoshi; Takahashi, Nobuhiro

doi:10.1155/2012/609689

Cited by 19 publications

(16 citation statements)

References 18 publications

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“…Another study found a positive association between P. micra and severe gingival overgrowth in patients taking cyclosporin A following organ transplantation, where P. micra was found in 66% of subgingival samples (Romito et al, 2004). Parvimonas micra was found to be present in 5.9% of isolates in a study looking at the profiling of the microbiota in infected root canals (Sato et al, 2012). Sato et al used restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal RNA genes and sequencing, for identification of live bacterial cells.…”

Section: Clinical Importancementioning

confidence: 99%

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

2013

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Among the Gram-positive anaerobic bacteria associated with clinical infections, the Gram-positive anaerobic cocci (GPAC) are the most prominent and account for approximately 25-30% of all isolated anaerobic bacteria from clinical specimens. Still, routine culture and identification of these slowly growing anaerobes to the species level has been limited in the diagnostic laboratory, mainly due to the requirement of prolonged incubation times and time-consuming phenotypic identification. In addition, GPAC are mostly isolated from polymicrobial infections with known pathogens and therefore their relevance has often been overlooked. However, through improvements in diagnostic and in particular molecular techniques, the isolation and identification of individual genera and species of GPAC associated with specific infections have been enhanced. Furthermore, the taxonomy of GPAC has undergone considerable changes over the years, mainly due to the development of molecular identification methods. Existing species have been renamed and novel species have been added, resulting in changes of the nomenclature. As the abundance and significance of GPAC in clinical infections grow, knowledge of virulence factors and antibiotic resistance patterns of different species becomes more important. The present review describes recent advances of GPAC and what is known of the biology and pathogenic effects of Anaerococcus, Finegoldia, Parvimonas, Peptoniphilus and Peptostreptococcus, the most important GPAC genera isolated from human infections.

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Section: Clinical Importancementioning

confidence: 99%

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

2013

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“…Periapical periodontitis was diagnosed based on clinical features, for example, sensitivity (tenderness) to percussion/occlusion and radiographical findings, as described previously [1]. Selected teeth had clinically no obvious margin leakages, had enough coronal structure for adequate isolation with a rubber dam, and were free of periodontal pockets deeper than 4 mm.…”

Section: Methodsmentioning

confidence: 99%

Rapid Quantification of Bacteria in Infected Root Canals Using Fluorescence Reagents and a Membrane Filter: A Pilot Study on Its Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment

Sato

Yamaki

Ishida

et al. 2012

International Journal of Dentistry

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Objective. The bacterial examination has been performed during the course of the root canal treatment. In the present pilot study, the new developed method, using fluorescence reagents and a membrane filter, was applied to the detection and quantification of bacteria in infected root canals, in order to evaluate the outcomes of the treatment. Methods. Six infected root canals with periapical lesions from 5 subjects were included. Informed consent was obtained from all subjects (age ranges, 23–79 years). Samples from infected root canals were collected at the beginning of the treatment (termed #25 First), the end of the first day of treatment (termed #55 First), and the next appointment day (termed #55 Second). Then, the bacterial count (CFU) was measured using fluorescence reagents (4′,6′-diamidino-2-phenylindole and propidium iodide) and the polycarbonate membrane filter by Bioplorer. Results. The mean ± SD of CFU in the sample of “#25 First” was (1.0 ± 1.4) × 105. As the root canal treatment progressed, the CFU decreased as 7.9 × 103 (#55 First) and 4.3 × 102 (#55 Second). Conclusion. In the present pilot study, rapid detection and quantification of bacteria in infected root canals were found to be successfully performed using fluorescence reagents and a membrane filter (Bioplorer analysis).

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“…Proteolytic bacteria predominate in the root‐canal flora, which changes, over time, to a more anaerobic microbiota . Rupf et al.…”

Section: Disease Relationshipsmentioning

confidence: 99%

Interaction between endodontics and periodontics

Rotstein¹

2017

Periodontology 2000

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Endodontic-periodontal lesions present challenges to the clinician regarding diagnosis, treatment planning and prognosis. Etiologic factors, such as bacteria and viruses, as well as contributing factors, such as trauma, root resorptions, perforations, cracks and dental malformations, play an important role in the development and progression of such lesions. Treatment and prognosis of endodontic-periodontal lesions vary, depending on the etiology, pathogenesis and correct diagnosis of each specific condition. This chapter will appraise the interrelationship between endodontic and periodontal lesions and provide biological and clinical evidence for diagnosis, prognosis and decision-making in the treatment of these conditions.

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Cultivable Anaerobic Microbiota of Infected Root Canals

Cited by 19 publications

References 18 publications

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

Rapid Quantification of Bacteria in Infected Root Canals Using Fluorescence Reagents and a Membrane Filter: A Pilot Study on Its Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment

Interaction between endodontics and periodontics

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