Cultivable fungal community associated with the tropical orchid Dichaea andina

Alomía, Yasmin A.; Otero, J. Túpac; Jersáková, Jana; Stevenson, Pablo R.

doi:10.1016/j.funeco.2022.101158

Cited by 4 publications

(2 citation statements)

References 77 publications

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“…Si bien se encuentra una amplia diversidad de hongos endófitos en los tejidos vegetales (Barolo et al, 2023;Alomía et al, 2022;Wu et al, 2020;Rojas et al, 2020;Novotná et al, 2018;Scott et al, 2018;Arnold et al, 2003), no todos tendrán potencial como agentes de biocontrol. Para evaluar el potencial biocontrol de estos microorganismos, se parte de la selección del material vegetal, aislamiento e identificación de los hongos endófitos, y posteriormente se realizan ensayos in vitro, los cuales se basan principalmente en cultivos duales (Figura 2) (Peters et al, 2020;Villavicencio-Vásquez et al, 2018;Manosalva y Mosquera-Espinosa, 2014).…”

Section: Hongos Endófitos Como Agentes De Biocontrol (Acb)unclassified

Biocontrol de Fusarium spp. en el cultivo de vainilla: Un nuevo modelo de estudio

Franco-Galindo,

Mosquera-Espinosa

2023

Temas Agrarios

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Biological control is a potential and sustainable option to deal with crop production and yield problems affected by insects or pathogenic microorganisms. In vanilla crop, obtaining the natural scent present in its fruits is limited, mainly by diseases that affect commercial crops. The main disease of vanilla crop is root and stem rot (RSR), caused by F. oxysporum f. sp. radicis-vanillae and F. oxysporum f. sp. vanillae. We found that the use of endophytic fungi (EF) isolated from healthy or asymptomatic plants requires systematic research for the selection of the most effective species to control pathogens, particularly in this crop. Additionally, there is no evidence reported with respect to this topic, in spite of, the use of these microorganisms is a natural alternative in other crops, compared to the use of agrochemicals used in traditional agriculture. This review addresses the perspective, and raises the need for research on the potential use of endophytic fungi as biological control agents of fungal pathogens in cultivated plants, emphasizing the relationship Endophytic fungi-Vanilla-pathogenic Fusarium.

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Section: Hongos Endófitos Como Agentes De Biocontrol (Acb)unclassified

Biocontrol de Fusarium spp. en el cultivo de vainilla: Un nuevo modelo de estudio

Franco-Galindo,

Mosquera-Espinosa

2023

Temas Agrarios

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“…The internal transcribed spacers of the rDNA gene (ITS1-5.8S-ITS2) have become the most commonly sequenced region for the identification of fungi due to its high variation [31]. Besides mycorrhizal fungi, culture-independent techniques also identify endophytes with roles in plant nutrition not well studied yet, mostly recognized as fungal pathogens, but with increasing evidence of benefits for plant protection and growth, even in some epiphytic orchids (see [32]). When applied alone, the culture-independent approach suffers from biases [33], such as primer mismatches [34] or primer cross-contaminations [35].…”

Section: Introductionmentioning

confidence: 99%

Diversity of Mycorrhizal Fungi in Temperate Orchid Species: Comparison of Culture-Dependent and Culture-Independent Methods

Mennicken,

Paula,

Vogt-Schilb

et al. 2024

JoF

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Many orchid species are endangered due to anthropogenic pressures such as habitat destruction and overharvesting, meanwhile, all orchids rely on orchid mycorrhizal fungi (OMF) for seed germination and seedling growth. Therefore, a better understanding of this intimate association is crucial for orchid conservation. Isolation and identification of OMF remain challenging as many fungi are unculturable. In our study, we tested the efficiency of both culture-dependent and culture-independent methods to describe OMF diversity in multiple temperate orchids and assessed any phylogenetic patterns in cultivability. The culture-dependent method involved the cultivation and identification of single pelotons (intracellular hyphal coils), while the culture-independent method used next-generation sequencing (NGS) to identify root-associated fungal communities. We found that most orchid species were associated with multiple fungi, and the orchid host had a greater impact than locality on the variability in fungal communities. The culture-independent method revealed greater fungal diversity than the culture-dependent one, but despite the lower detection, the isolated fungal strains were the most abundant OMF in adult roots. Additionally, the abundance of NGS reads of cultured OTUs was correlated with the extent of mycorrhizal root colonization in orchid plants. Finally, this limited-scale study tentatively suggests that the cultivability character of OMF may be randomly distributed along the phylogenetic trees of the rhizoctonian families.

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Orchid Mycorrhizas in South America: Tropical and Subtropical Ecosystems

Alomía

Otero

2022

Fungal Biology

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Cultivable fungal community associated with the tropical orchid Dichaea andina

Cited by 4 publications

References 77 publications

Biocontrol de Fusarium spp. en el cultivo de vainilla: Un nuevo modelo de estudio

Biocontrol de Fusarium spp. en el cultivo de vainilla: Un nuevo modelo de estudio

Diversity of Mycorrhizal Fungi in Temperate Orchid Species: Comparison of Culture-Dependent and Culture-Independent Methods

Orchid Mycorrhizas in South America: Tropical and Subtropical Ecosystems

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