Cultivar Identification Technique Applied for Hoshi-imo, Steamed and Dried Sweetpotato Slices, Based on Retrotransposon Insertion Polymorphisms

Ooe, Natsuko; Tahara, Makoto; Yamashita, Hiroki; Marutani, Yuu; Kuranouchi, Toshikazu

doi:10.1270/jsbbr.6.169

Cited by 4 publications

(1 citation statement)

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“…The use of retrotransposons for cultivar discrimination has been reported for rice (Oryza sativa) (Fukuchi et al 1993), dried sweet potato (Ipomoea batatas), which is made by heating sweet potato (Ooe et al 2004), and red bean paste, which is a heat-treated product of the adzuki bean (Vigna angularis) (Yamashita et al 2008).…”

Section: Introductionmentioning

confidence: 99%

DNA markers based on retrotransposon insertion polymorphisms can detect short DNA fragments for strawberry cultivar identification

et al. 2020

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In this study, DNA markers were developed for discrimination of strawberry (Fragaria × ananassa L.) cultivars based on retrotransposon insertion polymorphisms. We performed a comprehensive genomic search to identify retrotransposon insertion sites and subsequently selected one retrotransposon family, designated CL3, which provided reliable discrimination among strawberry cultivars. Through analyses of 75 strawberry cultivars, we developed eight cultivar-specific markers based on CL3 retrotransposon insertion sites. Used in combination with 10 additional polymorphic markers, we differentiated 35 strawberry cultivars commonly cultivated in Japan. In addition, we demonstrated that the retrotransposon-based markers were effective for PCR detection of DNA extracted from processed food materials, whereas a SSR marker was ineffective. These results indicated that the retrotransposon-based markers are useful for cultivar discrimination for processed food products, such as jams, in which DNA may be fragmented or degraded.

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Section: Introductionmentioning

confidence: 99%

DNA markers based on retrotransposon insertion polymorphisms can detect short DNA fragments for strawberry cultivar identification

et al. 2020

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Sweet Potato

Shimada¹,

Otani²

Transgenic Crops IV

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Effect of floral morphology on pollination in Brassica rapa L

Syafaruddin

Horisaki²,

Niikura³

et al. 2006

Euphytica

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Flower structure, especially the anther-stigma separation (ASS), is well known to affect pollination efficiency, and thus to potentially increase or decrease seed production in crops. Therefore, investigating the relationship between flower characteristics and pollination ability is crucial to a full understanding of mechanisms to improve F 1 seed production in Brassica rapa. We used image analysis to measure three flower characteristics: short stamen height (SSH); long stamen height (LSH); and pistil height (PH) in seven cultivars. We calculated the ratio of PH to LSH as an index of anther-stigma separation (ASS). We investigated the number of pollen grains (NPG) deposited on the stigma and the seed-set percentage (SSP) under open-pollination and self-pollination conditions (with and without insects, respectively). Nested ANOVA indicated significant differences between the seven cultivars in the floral characteristics except for PH. Moreover, much larger variation was observed in NPG and SSP than in floral characteristics. Although stepwise multiple regression analysis indicated that plants with relatively high PHs produced more seed under self-pollination, the number of seeds that resulted from self-pollination did not affect seed production because of incompatibility. Therefore, the effect of the spatial position of pistils on the F 1 seed production was low. Possibly other factors such as the total pollen production and visiting times of pollinators were important factors in the low yields observed in some cultivars.

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Cultivar Identification Technique Applied for Hoshi-imo, Steamed and Dried Sweetpotato Slices, Based on Retrotransposon Insertion Polymorphisms

Cited by 4 publications

References 18 publications

DNA markers based on retrotransposon insertion polymorphisms can detect short DNA fragments for strawberry cultivar identification

DNA markers based on retrotransposon insertion polymorphisms can detect short DNA fragments for strawberry cultivar identification

Sweet Potato

Effect of floral morphology on pollination in Brassica rapa L

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