Cultivation and bioactivity assessment of Grifola frondosa fruiting bodies on olive oil press cakes substrates

Abstract

Grifola frondosa, a medicinal and edible mushroom, was cultivated on substrates composed of olive oil press cakes with different supplements. Crude extracts were prepared from mature fruiting bodies and tested for their capacity to stimulate splenocyte proliferation. Two wild-growing fruiting bodies were extracted for comparison. Olive oil press cakes reduced the mushroom yield, and the best biological efficiency was obtained on substrates supplemented with wheat bran and without olive oil press cakes. All extracts were capable of inducing splenocyte proliferation and were half as effective as the positive control (6.0 microg/mL phytohaemagglutinin). No correlation between substrate composition and bioactivity could be established. Extracts from wild-growing G. frondosa were superior to cultivated ones in respect to biological activity.

Influence of Olive Oil Press Cakes on Shiitake Culinary-Medicinal Mushroom, Lentinus edodes (Berk.) Singer (Higher Basidiomycetes) Fruiting Bodies Production and Effect of their Crude Polysaccharides on CCRF-CEM Cell Proliferation

“…Strain characteristics tend to strongly influence mycelial growth as well as quantity and quality of produced fruiting bodies. 26,27 Reduction of fruiting bodies yields on substrates containing higher proportions of OOPC is in accordance with the findings of other authors, who tested OOPC as a substrate component for cultivation of other mushroom species, such as Grifola frondosa, 17 Pleurotus pulmonarius, 18 P. ostreatus, 19 and Ganoderma lucidum. For a better understanding of the influence the substrate has on fruiting bodies yield and mycelium growth rate, different fungal strains should be used.…”

“…16 They also appear to be a suitable source of nutrients for fruiting bodies cultivation. OOPC, being the major waste product in olive oil production, have been successfully used as a cultivating substrate component for different mushroom species, such as Grifola frondosa, 17 Pleurotus pulmonarius, 18 P. ostreatus, 19 and Ganoderma lucidum. In our study, we have cultivated L. edodes on substrates supplemented with different proportions of OOPC.…”

Research progress in submerged mycelial culture of Grifola frondosa, a culinary-medicinal mushroom

“…Extracts from wild-growing G. frondosa were superior to cultivated ones in respect to biological activity. Gregori et al, 2009. Table 3.…”

Growth Characteristics and Ergosterol Content of Grifola frondosa in Various Solid-state Substrates

“…Periodical mixing (80 rpm) by horizontal blades attached to a central shaft, for two minutes/day, and aeration over a hollow shaft of the impeller (5 L h -1 air flow) were applied. The same principles of process optimization as inoculation, sterilization, mixing, aeration, temperature, and humidity were applied as in our previous works 6,10 .…”

“…Grifola frondosa mycelium and its polysaccharides can be produced in liquid media 2 as well as solid-state cultivation on sunflower seed huls 3 and different grain types 3,4 , while fruiting bodies are usually cultivated on broadleaf sawdust-based substrates with addition of different supplements, such as wheat bran, crushed cord seeds 5 , olive oil press cakes 6 and others. Commercially, polypropylene bags or bottles are used as substrate containers from which fruiting bodies emerge.…”