Cultivation and characteristics of partial nitrification granular sludge in a sequencing batch reactor inoculated with heterotrophic granules

Wang, Jianfang; Qian, Feiyue; Liu, Xiaopeng; Liu, Wenru; Wang, Shuyong; Shen, Yun

doi:10.1007/s00253-016-7797-9

Cited by 19 publications

(6 citation statements)

References 42 publications

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“…Additionally, it was easy to distinguish them, and it was found that this was typical bacteria for the different functioning bacteria. For the phosphorus removal bacteria, they were short and rod-shaped, which could be seen directly from the SEM observations, which was similar to Wang et al's experiment [36]. It was observed that the abundances of Betaproteobacteria and Alphaproteobacteria were the highest in the bacteria community (the same as our results), and their SEM observations had large amounts of rod-shaped bacteria.…”

Section: Cod Removalssupporting

confidence: 91%

“…Dechloromonas (3.74% and 2.48%) and Simplicispira (4.47% and 3.21%) were the most common DPAOs for the biological wastewater treatment, which could use NO 3 − -N as the electron acceptor in an anoxic environment for oxidized organic matter [41][42][43], and their abundances both decreased after 0.5 h of the critical aerobic phase. Zoogloea was an easily adapted bacteria because they could adjust themselves either in an aerobic environment or anoxic environment for nitrifying and denitrifying [36,44]. Although the unique phenomenon had not occurred after 0.5 h, the abundance of Zoogloea was mildly enhanced (from 4.87% to 5.77%), which guaranteed the DNRA for NO 3 − -N removal [26].…”

Section: Sludge Structure and Microbial Community Compositionmentioning

confidence: 99%

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Optimization of Wastewater Phosphorus Removal in Winter Temperatures Using an Anaerobic–Critical Aerobic Strategy in a Pilot-Scale Sequencing Batch Reactor

Liu

Yao

Cong

et al. 2019

Water

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Biological phosphorus removal using an anaerobic-aerobic sequencing batch reactor (SBR) in a low temperature can be difficult to remove, and aeration always accounts for nearly half of the total electricity costs at many wastewater treatment plants. In this study, a pilot-scale anaerobic-critical aerobic SBR (A-CA SBR) was developed for synthetic domestic wastewater. More importantly, the phase, whose concentration of diffused oxygen was controlled at 1.0-1.5 mg/L, was defined as a critical aerobic phase, which reduced expenses during the operation. To be specific, half of the ammonia was removed within 10 days and no NO 3 − -N was accumulated during the process.From the SEM and metagenome analysis, Rhodocyclus, Zooglea, Dechloromonas, and Simplicispira had the ability to remove phosphorus and NO 3 − -N simultaneously, which proved the existence of a potential double-layer sludge structure under an A-CA operational condition. All of the results disclose that the pilot-scale A-CA SBR is a reliable manipulation strategy for phosphorus removal under low temperatures, which can hopefully apply to practical wastewater remediation.Among all of the influenced parameters, temperature is a vital factor in the phosphorus uptake process. Li et al. reported that 20 • C or possibly lower was suitable for P removal of microorganisms [2], and Liu and Li showed that temperatures below the optimum range had more significant effects on growth rate than those above the optimum range, because the activities of the microorganisms sharply decreased with the decreasing temperature. So, it was more difficult to realize phosphorus removal at a low temperature [10]. Additionally, Liu et al. [11] investigated the influence of temperature on denitrifying phosphorus removal (DPR), and found that more than 80% of phosphorus removal from DPR was obtained in the range of 20-30 • C, and the removal efficiencies went down when the temperature was below 10 • C. However, in the north of China, temperature changes drastically when the season varies. Especially in winter, wastewater is at low temperatures (11-13 • C) for a long period, which results in a negative impact on the performance of phosphorus removal [12]. Therefore, it is crucial to find a solution to treat wastewater in low temperatures properly.It has been noted that most studies have been focused on lab-scale SBR [3,7,13,14], and pilot-scale SBR are reported on less. In this study, the phase whose concentration of diffused oxygen was controlled at 1.0-1.5 mg/L was defined as a critical aerobic phase. A pilot-scale anaerobic-critical aerobic (A-CA) SBR, which had a good adaption, high efficiency, and strong practicality [15], was designed to remove phosphorus in wastewater under cold temperatures. During the operation process, a sludge structure of the inner anoxic zone and outer-critical aerobic layer was found, and metagenome analysis proved the microorganism's variation. In addition, the optimization of the length of the critical aerobic phase was investigated in order further to obta...

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Section: Cod Removalssupporting

confidence: 91%

Section: Sludge Structure and Microbial Community Compositionmentioning

confidence: 99%

Optimization of Wastewater Phosphorus Removal in Winter Temperatures Using an Anaerobic–Critical Aerobic Strategy in a Pilot-Scale Sequencing Batch Reactor

Liu

Yao

Cong

et al. 2019

Water

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“…Four representative granule samples (S1-S4) belonging to the different size fractions (Fractions I-IV) were obtained for the microbial community analysis, which was conducted by highthroughput pyrosequencing using MiSeq technology (Illumina, US), as described by Wang et al [20]. In order to enhance the DNA extraction, the different size granules were preprocessed by ultrasound at 1 W/ml for 5 min in an ice bath, respectively [18].…”

Section: Dna Extraction Pcr and Pyrosequencingmentioning

confidence: 99%

Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor

Qian¹,

Chen²,

Wang³

et al. 2017

Journal of Microbiology and Biotechnology

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The differentiations in nitrogen-converting activity and microbial community structure between granular size fractions in a continuous completely autotrophic nitrogen removal over nitrite (CANON) reactor, having a superior specific nitrogen removal rate of 0.24 g/(g VSS·d), were investigated by batch tests and high-throughput pyrosequencing analysis, respectively. Results revealed that a high dissolved oxygen concentration (>1.8 mg/l) could result in efficient nitrite accumulation with small granules (0.2-0.6 mm in diameter), because aerobic ammonium-oxidizing bacteria (genus ) predominated therein. Meanwhile, intermediate size granules (1.4-2.0 mm in diameter) showed the highest nitrogen removal activity of 40.4 mg/(g VSS·h) under sufficient oxygen supply, corresponding to the relative abundance ratio of aerobic to anaerobic ammonium-oxidizing bacteria (genus Kuenenia) of 5.7. Additionally, a dual substrate competition for oxygen and nitrite would be considered as the main mechanism for repression of nitrite-oxidizing bacteria, and the few spp. did not remarkably affect the overall performance of the reactor. Because all the granular size fractions could accomplish the CANON process independently under oxygen limiting conditions, maintaining a diversity of granular size would facilitate the stability of the suspended growth CANON system.

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“…However, it is not always the case that the low air pressure facilitated the high SND efficiency. The previous experimental results show that the specific NH 4 + ‐N removal rate increased from 4.98 to 9.26 g/(m 2 d) when air pressure increased from 2 to 20 kPa in an MABR with a biofilm thickness of approximately 600 μm . In addition, Li et al .…”

Section: Resultsmentioning

confidence: 79%

Application of a composite membrane aerated biofilm with controllable biofilm thickness in nitrogen removal

Zeng

Yang

Wang

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: The manipulation of aeration and biofilm thickness remain two key factors for membrane aerated biofilm. A composite membrane aerated biofilm (CMAB) reactor coated with microorganisms embedded in hydrogel was developed to quantitatively control the biofilm thickness by introducing the microbial immobilization technique. RESULTS: Results show that the thin gel film exhibited high dispersion coefficients of oxygen and substrates, and the crosslinking solution of sodium nitrate (NaNO 3 ) contributed to the highest specific ammonium-nitrogen (NH 4+ -N) removal rate of 615.33 mg/(gVSS d) with respect to partial nitrification. Correspondingly, the microbial activity of this thin gel film was also the strongest. Therefore, with the simultaneous nitrification and denitrification, the gel thickness and air pressure co-determined its efficiency. The optimum biofilm thickness and air pressure was 0.1 mm and 1 kPa, respectively, achieving the nitrogen removal rate of 1594.95 mg/(gVSS d). Furthermore, two abundant denitrifiers of Rhizobium and Thauera dominated the bacterial community of all four samples, and their proportion varied according to the aeration condition. Nitrosomonas tended to enrich in the thin CMAB under low air pressure and few Nitrospira existed.CONCLUSION: The thickness of developed CMAB can be quantitatively controlled, and the precise regulation of simultaneous nitrification and denitrification might be achieved by adjusting the gel film thickness and air pressure. The CMAB is supposed to greatly facilitate the application of membrane aerated biofilm for nitrogen removal.

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Cultivation and characteristics of partial nitrification granular sludge in a sequencing batch reactor inoculated with heterotrophic granules

Cited by 19 publications

References 42 publications

Optimization of Wastewater Phosphorus Removal in Winter Temperatures Using an Anaerobic–Critical Aerobic Strategy in a Pilot-Scale Sequencing Batch Reactor

Optimization of Wastewater Phosphorus Removal in Winter Temperatures Using an Anaerobic–Critical Aerobic Strategy in a Pilot-Scale Sequencing Batch Reactor

Differentiation in Nitrogen-Converting Activity and Microbial Community Structure between Granular Size Fractions in a Continuous Autotrophic Nitrogen Removal Reactor

Application of a composite membrane aerated biofilm with controllable biofilm thickness in nitrogen removal

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