Cultivation and characterization of snowbound microorganisms from the South Pole

Hayward, Mackenzie K.; Dewey, Emma D.; Shaffer, Kathryn N.; Huntington, Austin M; Burchell, Brad M.; Stokes, Lynn M.; Alexander, Brittney C.; George, Janessa E.; Kempher, Megan L.; Joye, Samantha B.; Madigan, Michael T.; Sattley, W. Matthew

doi:10.1007/s00792-021-01218-z

Cited by 8 publications

(6 citation statements)

References 71 publications

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“…Apart from extreme cold, the organisms that survive in polar regions have to adapt to desiccation, strong winds, low water availability, high UV radiation, multiple freeze–thaw cycles, etc. (Hayward et al 2021 ). Biodiversity recognized from the polar regions is still limited because of the extremely harsh conditions.…”

Section: Introductionmentioning

confidence: 99%

Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Ren

Ding

et al. 2022

Bioresour. Bioprocess.

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The polar psychrotrophic fungus Geomyces sp. WNF-15A can produce high-quality natural red pigment for the potential use as edible pigment. However, it shows low-temperature-dependent synthesis of red pigment, which limits its large-scale industrial applications due to the difficult and high-cost bioprocess control. This study aims to develop transposon-mediated mutagenesis methods to generate mutants that are able to synthesize red pigment at normal temperature. Four transposable systems, including single and dual transposable systems, were established in this fungus based on the Minos from Drosophila hydei and the Restless from Tolypocladium inflatum. A total of 23 production-dominant mutants and 12 growth-dominant mutants were thus obtained by constructed transposable systems. At 14 °C and 20 °C, the MPS1 mutant strain achieved the highest level of red pigment (OD520 of 43.3 and 29.7, respectively), which was increased by 78.4% and 128.7% compared to the wild-type, respectively. Of note, 4 mutants (MPS1, MPS3, MPS4 and MPD1) successfully synthesized red pigment (OD520 of 5.0, 5.3, 4.7 and 4.9, respectively) at 25 °C, which broke the limit of the wild-type production under normal temperature. Generally, the dual transposable systems of Minos and Restless were more efficient than their single transposable systems for mutagenesis in this fungus. However, the positive mutation ratios were similar between the dual and single transposable systems for either Minos or Restless. This study provides alternative tools for genetic mutagenesis breeding of fungi from extreme environments. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Ren

Ding

et al. 2022

Bioresour. Bioprocess.

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“…For instance, R. mucilaginosa was isolated from EE, such as the Peninsula (Troncoso et al 2017 ), Antarctic sea (Wang et al 2019 ), oilfield (Derguine-Mecheri et al 2021 ), and Sua pan (Loeto et al 2021 ). Similarly, N. albida has been isolated from Antarctic soil samples (Białkowska et al 2017 ), snow from the south pole (Hayward et al 2021 ), and hypersaline coastal waters (Fotedar et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Raklami,

Babalola,

Jemo

et al. 2024

FEMS Microbiology Letters

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In this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their ITS regions. Our research thoroughly characterizes plant growth-promoting (PGP) abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa’s growth. Four treatments were employed: i) control without inoculation (NI), ii) inoculation with L1, iii) inoculation with L2, and iv) inoculation with the mixture L1+L2. L1 isolated from Toubkal Mountain shared 99.83% sequence similarity to Rhodotorula mucilaginosa. Meanwhile, L2, thriving in the arid Merzouga desert, displayed a similar identity to Naganishia albida (99.84%). Yeast strains were tolerant to NaCl (2 M) and 60% PEG (polyethylene glycol P6000) in case of drought. Both strains could solubilize P and K (in the case of L2), produce IAA (up to 135 µl mL−1), have siderophores ability, and produce ACC deaminase. Isolates L1, L2, and their consortium showed that the single or combined strains inoculation of M. sativa improved plant growth, development, and nutrient assimilation. Those findings pave the way for harnessing yeast-based solutions in agricultural practices, contributing to enhanced crop productivity and environmental sustainability.

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“…Several Bacteria are capable of subzero growth, and microbial communities may exist in some of Earth's coldest environments, such as the interior of Antarctica (Madigan et al 2017;Hayward et al 2021). This indicates that extreme cold is not an insurmountable barrier for terrestrial (and presumably extraterrestrial) life.…”

Section: 'Ecophysiology Of Extremophiles' Special Issuementioning

confidence: 99%

“…2017; Hayward et al . 2021). This indicates that extreme cold is not an insurmountable barrier for terrestrial (and presumably extraterrestrial) life.…”

Section: ‘Ecophysiology Of Extremophiles’ Special Issuementioning

confidence: 99%

Astrobiology of life on Earth

Hallsworth

Mancinelli

Conley

et al. 2021

Environmental Microbiology

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Summary Astrobiology is mistakenly regarded by some as a field confined to studies of life beyond Earth. Here, we consider life on Earth through an astrobiological lens. Whereas classical studies of microbiology historically focused on various anthropocentric sub‐fields (such as fermented foods or commensals and pathogens of crop plants, livestock and humans), addressing key biological questions via astrobiological approaches can further our understanding of all life on Earth. We highlight potential implications of this approach through the articles in this Environmental Microbiology special issue ‘Ecophysiology of Extremophiles’. They report on the microbiology of places/processes including low‐temperature environments and chemically diverse saline‐ and hypersaline habitats; aspects of sulphur metabolism in hypersaline lakes, dysoxic marine waters, and thermal acidic springs; biology of extremophile viruses; the survival of terrestrial extremophiles on the surface of Mars; biological soils crusts and rock‐associated microbes of deserts; subsurface and deep biosphere, including a salticle formed within Triassic halite; and interactions of microbes with igneous and sedimentary rocks. These studies, some of which we highlight here, contribute to our understanding of the spatiotemporal reach of Earth'sfunctional biosphere, and the tenacity of terrestrial life. Their findings will help set the stage for future work focused on the constraints for life, and how organisms adapt and evolve to circumvent these constraints.

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Cultivation and characterization of snowbound microorganisms from the South Pole

Cited by 8 publications

References 71 publications

Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming

Astrobiology of life on Earth

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