Cultivation and characterization of symbiotic bacteria from the gut of Reticulitermes chinensis

Abstract

Cultivation and characterization of symbiotic bacteria from the gut of Reticulitermes chinensis.

Dynamics of the intestinal bacterial community in black soldier fly larval guts and its influence on insect growth and development

“…Two other bacteria ( Citrobacter and Ochrobacterium ) that were not very abundant in the guts of BSFL also promote BSF growth and development. Citrobacter is frequently detected and isolated from the guts of various insects, like termites, BSF, and the oriental fruit fly (Fang et al ., 2016; Guo et al ., 2017; Cai et al ., 2018). Previous studies have shown that Citrobacter promotes cellulose degradation (Fontes‐Perez et al ., 2015) and enhances the resistance of the oriental fruit fly to insecticides (Cheng et al ., 2017).…”

“…The PCR mixture and amplification procedure were as described by Fang et al . (2016). Strains were classified using amplified ribosomal DNA restriction analyses.…”

“…Providencia (BSF1), Klebsiella (BSF3), and Dysgonomonas (BSF6) were predominant in almost all the larval instars, and have also been found in the intestines of BSFL under different rearing conditions (Zheng et al, 2013a;Cifuentes et al, 2020;Klammsteiner et al, 2021). Citrobacter (BSF2), Proteus (BSF4), and Ochrobactrum (BSF5) were not very abundant in different larval instars but were frequently isolated from BSF and other insect intestines (Fang et al, 2016;Cheng et al, 2017;Cai et al, 2018;Mazza et al, 2020). The characteristics of carbon utilization and the enzyme activities of BSF1-BSF6 were determined using an API 50CH fermentation kit (bioMérieux) and an API ZYM The enzyme activity tests showed that BSF1 and BSF3-BSF5 have higher esterase activities than BSF2 and BSF6, whereas BSF2, BSF3, and BSF6 have more enzyme activities associated with cellulose degradation than BSF1 and BSF4 (Table 4).…”

“…The universal bacterial primers 27F (5 -AGAGTTTGATCCTGGCTCAG-3 ) and 1492R (5 -TACGGYTACCTTGTTACGACTT-3 ) (Weisburg et al, 1991) were used for the amplification of the 16S rDNAs of isolated strains. The PCR mixture and amplification procedure were as described by Fang et al (2016). Strains were classified using amplified ribosomal DNA restriction analyses.…”

Yokenella regensburgei—Past, Present and Future

“…Subsequent studies investigating the reservoir of Y. regensburgei have increased the understanding of its animal reservoir to include insects such as southern green stink bug (Nezara viridula), brown stink bug (Euschistus heros), giant spiny stick insect (Eurycantha calcarata), termites (Reticulitermes chinensi), boxelder bug (Boisea trivittata). Addditionally, Y. regensburgei has also been isolated from soil samples in China, thus greatly expanding its known natural reservoir [15,[18][19][20][21][22][23]. However, veterinary infections produced by this bacterium were not reported until recently.…”