Cultivation and properties of bovine mammary cell cultures

Ebner, Kurt E.; Hoover, Charles R.; Hageman, Elizabeth C.; Larson, B.L.

doi:10.1016/0014-4827(61)90046-5

Cited by 68 publications

(4 citation statements)

References 6 publications

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“…The cells were prepared from fresh lactating tissue obtained from Holstein cows immediately post mortem. The method of preparation, washing, and dispersal has been thoroughly described (8,9). The composition of the culture media and the incubation and harvesting methods have been reported (9).…”

Section: Methodsmentioning

confidence: 99%

The absorption of fatty acids by functional bovine mammary cells

Kinsella

1970

Lipids

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Freshly dispersed bovine mammary cells rapidly absorbed long chain fatty acids from the culture medium. Differences in the rates of absorption were observed, i.e., palmitic > stearic > oleic > myristic > linoleic acid. The preponderance of the fatty acids absorbed were esterified into triglycerides (>75%) and the remainder were mostly incorporated into phospholipids. The cells secreted triglycerides into the culture medium. Of the phospholipid classes, phosphatidylcholine always contained most of the radioactivity in all experiments with labeled fatty acids. These observations are related to the metabolism of mammary cells in vivo.

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Section: Methodsmentioning

confidence: 99%

The absorption of fatty acids by functional bovine mammary cells

Kinsella

1970

Lipids

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“…The MECs used in culture models are broadly classified into primary cells and cell lines. Primary MECs are isolated from mammary glands of cows, sows, goats, mice, rats, rabbits, Guinea pigs, and humans [ 79 – 86 ]. They are commonly isolated by enzymatic treatment, mainly with collagenase.…”

Section: Overview Of Mec Culture Modelsmentioning

confidence: 99%

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

Kobayashi

2023

J Mammary Gland Biol Neoplasia

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Mammary epithelial cells (MECs) are the only cell type that produces milk during lactation. MECs also form less-permeable tight junctions (TJs) to prevent the leakage of milk and blood components through the paracellular pathway (blood-milk barrier). Multiple factors that include hormones, cytokines, nutrition, and temperature regulate milk production and TJ formation in MECs. Multiple intracellular signaling pathways that positively and negatively regulate milk production and TJ formation have been reported. However, their regulatory mechanisms have not been fully elucidated. In addition, unidentified components that regulate milk production in MECs likely exist in foods, for example plants. Culture models of functional MECs that recapitulate milk production and TJs are useful tools for their study. Such models enable the elimination of indirect effects via cells other than MECs and allows for more detailed experimental conditions. However, culture models of MECs with inappropriate functionality may result in unphysiological reactions that never occur in lactating mammary glands in vivo. Here, I briefly review the physiological functions of alveolar MECs during lactation in vivo and culture models of MECs that feature milk production and less-permeable TJs, together with a protocol for establishment of MEC culture with functional TJ barrier and milk production capability using cell culture inserts.

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“…Following initial characterization of isolation methods and culture procedures (Ebner et al, 1961), Larson (1965 reviewed the status of regulation of milk protein biosynthesis and especially the utility of results obtained by various culture techniques. Subsequently, Larson (1972) showed the stimulatory effect of methionine on milk protein synthesis, and Rao et al (1975) demonstrated the effects of cell density on synthesis of lactose by bovine mammary cells.…”

Section: Cell and Tissue Culturementioning

confidence: 99%

A 100-Year Review: Mammary development and lactation

Akers

2017

Journal of Dairy Science

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What is old is new again-and with respect to the study of the mammary development and function in dairy animals, the expression resonates. Many of the mammary and milk production questions raised in the early years of the Journal of Dairy Science apply today. To be sure, scientists have filled in many details regarding, for example, identification of hormones and growth factors important in the control of mammary growth, the onset of copious milk production at calving, and maintenance of lactation. Early years focused on identification and subsequent availability of classic mammogenic, lactogenic, and galactopoietic hormones (e.g., steroids, prolactin, and growth hormone). The advent of sensitive assays to measure concentrations of these hormones and, subsequently, myriad growth factors in blood, milk, and tissues, allowed creation of multiple hypotheses to explain mammary cell proliferation and regulation of function. It is also apparent that we understand many of the fundamentals of milk removal, milking frequency, milking management, and milk ejection for successful lactation. However, some questions remain. Are the principles that were identified when cows produced markedly less milk still valid for the high-producing cows of today and the future? What mechanism(s) explain the positive effects of early increased milking frequency on subsequent milk production? Can the persistency of lactation be improved (secretory cell number vs. secretory cell function) or does early management "program" future mammary development or productivity (epigenetics, immune responsiveness, other)? The explosion of tools and techniques (Southern and Northern blots, PCR, and the "-omics" revolution) has driven an almost overwhelming evaluation of cellular and molecular functions in the mammary gland and other tissues. One key may be the discovery of a "Rosetta stone" that will allow understanding of this mass of detailed information on gene expression, cell signaling, and so on. Many scientists can now better appreciate the difficulty of the dairy farmer seeking to process DHIA or Dairy Comp 305 data, milking data, weights, feeding reports, pedometer readings, or genomic evaluations to manage their operations.

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Cultivation and properties of bovine mammary cell cultures

Cited by 68 publications

References 6 publications

The absorption of fatty acids by functional bovine mammary cells

The absorption of fatty acids by functional bovine mammary cells

Culture Models to Investigate Mechanisms of Milk Production and Blood-Milk Barrier in Mammary Epithelial Cells: a Review and a Protocol

A 100-Year Review: Mammary development and lactation

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