Cultivation and Utilization of Shiitake Mushroom

Atíla, Funda

doi:10.1007/978-3-030-74779-4_13

Cited by 7 publications

(4 citation statements)

References 136 publications

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“…The cultivation of shiitake on sugarcane bagasse (Rossi et al) [17], sunflower husks [18], barley straw and vineyard pruning [19], maize cobs [20], chickpea straw, corn stalk, alfalfa hay [21], rice straw [22] and hardwood sawdust [23] as growing substrates was studied. Atila [24] reported that shiitake mushrooms show selectivity towards a growing substrate with a low cellulose/lignin ratio. Among the various agrowastes available in India, wheat straw is one potential substrate that has great significance in mushroom production due to its chemical composition and large availability [16].…”

Section: Introductionmentioning

confidence: 99%

“…Among the various agrowastes available in India, wheat straw is one potential substrate that has great significance in mushroom production due to its chemical composition and large availability [16]. Several researchers [19,21,24] reported that the lignocellulosic composition varies greatly depending on the cultivation process and will have a significant influence on the biological efficiency of the shiitake crop. Taking into account of these notable findings, we have attempted the cultivation of different strains of shiitake on both sawdust and wheat straw exposed to various degrees of heat treatment with the objective to find an optimum level of heat treatment to obtain the maximum biological efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Heat Treatment and Solid-State Fermentation on the Lignocellulosic Fractions of Substrates Supporting Lentinula edodes (Berk.) Pegler Cultivation: Implications for Commercial Production

et al. 2023

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Mushroom production in India has registered a considerable growth in the recent times. However, cultivation of shiitake mushroom, which represents a major share at a global level, is still at a primitive stage in the Indian subcontinent. The scarcity of raw materials and the cost of energy for substrate sterilization are the major hurdles for a large-scale production. The present study delves into the possibility of growing shiitake mushroom on lignocellulosic biomass (saw dust and wheat straw) processed with different heat treatments to develop a cost-effective production technology. Six different strains of shiitake mushroom, viz., DMRO-35, 51, 297, 388s, 410, 412, were used in this study. The substrates were exposed to a pasteurization temperature of 80 ± 5°C in a bulk pasteurization chamber for three different times (H1–H3) and also to a high-pressure sterilization (H4) in an autoclave. DMRO-388s was found to be the most productive strain, irrespective of the substrate and heat treatment method used. Significant differences were observed in the biological yield depending on the type of substrate and heat treatment. Changes in the biochemical composition of the lignocellulosic residues in three different stages, viz., pre heat treatment, inoculation and primordial formation stages, were recorded. Changes in heat treatment levels and duration significantly altered the cellulose/lignin ratio of the growing substrate. High-pressure sterilization aided the rapid degradation of lignin in the substrate and increased its bioavailability, thereby facilitating the fungus achieving its potential yield. A significant correlation in the positive direction between the yield levels of the tested strains and the consumption of lignin in the growing substrate was found, suggesting the significance of pre heat treatment for the bioconversion of lignin and its subsequent utilization in the solid-state fermentation process. The substrate pre heat treatment under high-pressure sterilization was proved to be beneficial to obtain the maximum yields of shiitake mushroom.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Heat Treatment and Solid-State Fermentation on the Lignocellulosic Fractions of Substrates Supporting Lentinula edodes (Berk.) Pegler Cultivation: Implications for Commercial Production

et al. 2023

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“…It is considered an essential ingredient for many Chinese and Japanese dishes [14]. Due to favorable climatic conditions, L. edodes cultivation is growing in Brazil using eucalypt logs, especially in the South and Southeastern regions [15]. L. edodes is ideal among functional mushrooms for the widespread research of its bioactivity, primarily the isolation of pure compounds which have important pharmaceutical use.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization and Biological Activities of Zinc Oxide Nanoparticles Derived from Secondary Metabolites of Lentinula edodes

et al. 2023

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Zinc oxide nanoparticles (ZnO NPs) are the second most prevalent metal oxide, owing to their characteristics of low cost, safe, and easily prepared. ZnO NPs have been found to exhibit unique properties which show their potential to be used in various therapies. Numerous techniques have been devised for the manufacture of zinc oxide because it is one of the nanomaterials that has received major research interest. Mushroom sources are proven to be efficient, ecologically friendly, inexpensive, and safe for humankind. In the current study, an aqueous fraction of methanolic extract of Lentinula edodes (L. edoes) was used to synthesize ZnO NPs. The biosynthesis of ZnO NPs was achieved by using the reducing and capping capability of an L. edodes aqueous fraction. Bioactive compounds from mushroom, such as flavonoids and polyphenolic compounds, are used in the green synthesis process to biologically reduce metal ions or metal oxides to metal NPs. Biogenically synthesized ZnO NPs were further characterized by using UV–Vis, FTIR, HPLC, XRD, SEM, EDX, zeta sizer and zeta potential analyses. The FTIR showed the functional group at the spectra in the range 3550–3200 cm−1 indicated the presence of the hydroxyl (OH) group, while bands in the range 1720–1706 cm−1 indicated C=O carboxylic stretches bonds. Furthermore, the XRD pattern of ZnO NPs created in the current study was found to be nanocrystals which are hexagonal. The SEM analysis of ZnO NPs showed spherical shapes and size distributions in the range 90–148 nm. Biologically synthesized ZnO NPs have substantial biological activities including antioxidant, antimicrobial, antipyretic, antidiabetic and anti-inflammatory potential. Biological activities showed significant antioxidant (65.7 ± 1.09), antidiabetic (85.18 ± 0.48), and anti-inflammatory potential (86.45 ± 0.60) at 300 µg inhibition in paw inflammation of (1.1 ± 0.06) and yeast-induced pyrexia (97.4 ± 0.51) at 10 mg in a dose-dependent manner. The outcomes of this research indicated that ZnO NPs significantly reduced inflammation and have the ability to scavenge free radicals and prevent protein denaturation, while also indicating their possible use in food and nutraceutical applications to treat various ailments.

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“…However, the cultivation of L. edodes takes a relatively long time, and there are great uncertainties with regard to yield and quality. The cultivation model of L. edodes has changed from logs to bags, shortening the cultivation time to some extent and increasing the yield of fruiting bodies [ 6 ]. In addition, extracting and purifying polysaccharides from liquid fermentation shows the potential for shortening the production cycle.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes

et al. 2022

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The polysaccharides found in Lentinula edodes have a variety of medicinal properties, such as anti-tumor and anti-viral effects, but their content in L. edodes sporophores is very low. In this study, Fe2+ was added to the liquid fermentation medium of L. edodes to analyze its effects on mycelial growth, polysaccharide and enzyme production, gene expression, and the activities of enzymes involved in polysaccharide biosynthesis, and in vitro antioxidation of polysaccharides. The results showed that when 200 mg/L of Fe2+ was added, with 7 days of shaking at 150 rpm and 3 days of static culture, the biomass reached its highest value (0.28 mg/50 mL) 50 days after the addition of Fe2+. Besides, Fe2+ addition also enhanced intracellular polysaccharide (IPS) and exopolysaccharide (EPS) productions, the levels of which were 2.98- and 1.79-fold higher than the control. The activities of the enzymes involved in polysaccharides biosynthesis, including phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), and UDPG-pyrophosphorylase (UGP) were also increased under Fe2+ addition. Maximum PGI activity reached 1525.20 U/mg 30 days after Fe2+ addition, whereas PGM and UGP activities reached 3607.05 U/mg and 3823.27 U/mg 60 days after Fe2+ addition, respectively. The Pearson correlation coefficient showed a strong correlation (p < 0.01) between IPS production and PGM and UGP activities. The corresponding coding genes of the three enzymes were also upregulated. When evaluating the in vitro antioxidant activities of polysaccharides, EPS from all Fe2+-treated cultures exhibited significantly better capacity (p < 0.05) for scavenging -OH radicals. The results of the two-way ANOVA indicated that the abilities of polysaccharides to scavenge O2− radicals were significantly (p < 0.01) affected by Fe2+ concentration and incubation time. These results indicated that the addition of iron provided a good way to achieve desirable biomass, polysaccharide production, and the in vitro antioxidation of polysaccharides from L. edodes.

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Cultivation and Utilization of Shiitake Mushroom

Cited by 7 publications

References 136 publications

Influence of Heat Treatment and Solid-State Fermentation on the Lignocellulosic Fractions of Substrates Supporting Lentinula edodes (Berk.) Pegler Cultivation: Implications for Commercial Production

Influence of Heat Treatment and Solid-State Fermentation on the Lignocellulosic Fractions of Substrates Supporting Lentinula edodes (Berk.) Pegler Cultivation: Implications for Commercial Production

Synthesis, Characterization and Biological Activities of Zinc Oxide Nanoparticles Derived from Secondary Metabolites of Lentinula edodes

Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides from Lentinula edodes

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