Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

Olsson, Joakim; Toth, Gunilla B.; Oerbekke, Annelous; Cvijetinovic, Suzana; Wahlström, Niklas; Harrysson, Hanna; Steinhagen, Sophie; Kinnby, Alexandra; White, Joel C. B.; Edlund, Ulrica; Undeland, Ingrid; Pavia, Henrik; Albers, Eva

doi:10.1007/s10811-020-02138-9

Cited by 19 publications

(28 citation statements)

References 38 publications

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“…Since glucuronic acid also is the main component of the glucuronan polysaccharide in U. intestinalis, the link between ulvan and geographical location cannot be confirmed, although the numerous indications lend the argument some merit. In defined tank cultivation of Ulva fenestrata, it was found that low levels of nitrate increased the content of iduronic acid and low levels of phosphate increased rhamnose content in biomass, while increased irradiance affected both the iduronic acid and rhamnose contents (Olsson et al, 2020b). It is thus apparent that ulvan content and subsequently monosaccharide content is highly variable and allows Ulva species to acclimate to changes in variation in environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Carbohydrate content and composition were determined after acid hydrolysis of 25 mg samples according to Bikker et al (2016) without neutralization, and subsequent analysis by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) (Olsson et al, 2020b). The total carbohydrate content was calculated as the sum of monosaccharides and sugar acids corrected for the addition of water during hydrolysis of polysaccharides.…”

Section: Analyses Of Biomass Componentsmentioning

confidence: 99%

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Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

et al. 2020

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Macroalgal biomass has the potential to become an important source of chemicals and commodities in a future biorefinery. Currently, production of macroalgal biomass is expensive and the content of high-value compounds is often low. Therefore, in this study the biochemical composition of Ulva intestinalis along the Swedish west coast and the east coast up to Stockholm was assessed with the aim of determining how the content of potentially valuable compounds, such as rhamnose, iduronic acid and PUFAs, could be maximized by utilizing natural variation in the choice of marine cultivation site. Along the investigated coastline, the salinity dropped from 19.4‰ at high latitudes along the west coast to 5.4‰ at Stockholm. Nitrogen and phosphorus availability varied, while temperature was similar at all locations. The two major components of biomass, carbohydrates and ash, varied inversely with the highest content of ash in the west and carbohydrates in the east. In addition, total fatty acids were significantly higher in west coast samples at 3.2 g 100 g-1 dw, with a higher proportion of mono-and polyunsaturated fatty acids. Some healthbeneficial fatty acids were found, including EPA and DPA, at 10-50 mg 100 g-1 dw, respectively. The metal content and elemental composition varied widely, probably due to the influence of specific local conditions. The P content was correlated with the phosphorus concentration in waters at the locations. In PCA analysis, the monosaccharides constituting the cell wall polysaccharide ulvan were found to vary by geographical location, with higher levels possibly associated with lower salinities. However, only glucuronic acid differed significantly between sites. These results show the considerable geographical variability in the composition of Swedish U. intestinalis and suggest that different salinities could be used to create a lipid-or carbohydrate-rich biomass.

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Section: Discussionmentioning

confidence: 99%

Section: Analyses Of Biomass Componentsmentioning

confidence: 99%

Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

et al. 2020

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“…Additionally, value-added products such as functional foods, cosmeceuticals, nutraceuticals and pharmaceuticals can be produced from their many bioactive compounds [34,35]. Ulva biomass can exhibit high total carbohydrate contents (15-65% dw) [27,36,37] and comprises the soluble sulphated polysaccharide ulvan. Ulvan can be used in water-conditioning hydrogels [11] and can be processed into heparin-like oligosaccharides as well as into rare monosaccharides, such as rhamnose and iduronic acid [10].…”

Section: Introductionmentioning

confidence: 99%

“…Ulvan can be used in water-conditioning hydrogels [11] and can be processed into heparin-like oligosaccharides as well as into rare monosaccharides, such as rhamnose and iduronic acid [10]. Recent studies have shown that environmental growth conditions have significant effects on the relative growth rate as well as on the biochemical composition of the abovenamed high-value compounds, e.g., [36][37][38][39][40] which underlines the importance of the optimization of cultivation conditions in aquaculture settings [40].…”

Section: Introductionmentioning

confidence: 99%

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Steinhagen

Enge

Larsson

et al. 2021

JMSE

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The growing world population demands an increase in sustainable resources for biorefining. The opening of new farm grounds and the cultivation of extractive species, such as marine seaweeds, increases worldwide, aiming to provide renewable biomass for food and non-food applications. The potential for European large-scale open ocean farming of the commercial green seaweed crop Ulva is not yet fully realized. Here we conducted manipulative cultivation experiments in order to investigate the effects of hatchery temperature (10 and 15 °C), nutrient addition (PES and 3xPES) and swarmer density (500 and 10,000 swarmers ml−1) on the biomass yield and biochemical composition (fatty acid, protein, carbohydrate, pigment and phenolic content) of off-shore cultivated Ulva fenestrata in a Swedish seafarm. High seedling densities were optimal for the growth of this northern hemisphere crop strain and significantly increased the mean biomass yield by ~84% compared to low seedling densities. Variations of nutrients or changes in temperature levels during the hatchery phase were not necessary to increase the subsequent growth in an open-water seafarm, however effects of the factors on the thallus habitus (thallus length/width) were observed. We found no significant effect of the environmental factors applied in the hatchery on the total fatty acid or crude protein content in the off-shore cultivated Ulva. However, low seedling density and low temperature increased the total carbohydrate content and furthermore, high temperature in combination with high nutrient levels decreased the pigment content (chlorophyll a, b, carotenoids). Low temperature in combination with high nutrient levels increased the phenolic content. Our study confirms the successful and sustainable potential for large-scale off-shore cultivation of the Scandinavian crop U. fenestrata. We conclude that high seedling density in the hatchery is most important for increasing the total biomass yield of sea-farmed U. fenestrata, and that changing temperature or addition of nutrients overall does not have a large effect on the biochemical composition. To summarize, our study contributes novel insights into the large-scale off-shore cultivation potential of northern hemisphere U. fenestrata and underpins suitable pre-treatments during the hatchery phase of seedlings to facilitate a successful and cost-efficient large-scale rope cultivation.

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“…The overall aim of the present study was to investigate how changes in several cultivation conditions (irradiance, temperature, nitrate, phosphate, and pCO 2 ) affect the growth and levels of several commonly measured biochemical constituents (total fatty acid, protein, phenolics, ash, and biochar content) of Swedish U. fenestrata in the same study. Effects of environmental factors on the carbohydrate and ulvan content of U. fenestrata are presented in Olsson et al (2020). To achieve our aim, we conducted three manipulative experiments where the interactive and/or single effects of (1) irradiance and temperature, (2) nitrate and phosphate, and (3) pCO 2 at different levels were tested in indoor cultivation tanks with flow-through seawater.…”

Section: Introductionmentioning

confidence: 99%

Effects of irradiance, temperature, nutrients, and pCO2 on the growth and biochemical composition of cultivated Ulva fenestrata

et al. 2020

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Ulva fenestrata is an economically and ecologically important green algal species with a large potential in seaweed aquaculture due to its high productivity, wide environmental tolerance, as well as interesting functional and nutritional properties. Here, we performed a series of manipulative cultivation experiments in order to investigate the effects of irradiance (50, 100, and 160 μmol photons m−2 s−1), temperature (13 and 18 °C), nitrate (< 5, 150, and 500 μM), phosphate (< 1 and 50 μM), and pCO2 (200, 400, and 2500 ppm) on the relative growth rate and biochemical composition (fatty acid, protein, phenolic, ash, and biochar content) in indoor tank cultivation of Swedish U. fenestrata. High irradiance and low temperature were optimal for the growth of this northern hemisphere U. fenestrata strain, but addition of nutrients or changes in pCO2 levels were not necessary to increase growth. Low irradiance resulted in the highest fatty acid, protein, and phenolic content, while low temperature had a negative effect on the fatty acid content but a positive effect on the protein content. Addition of nutrients (especially nitrate) increased the fatty acid, protein, and phenolic content. High nitrate levels decreased the total ash content of the seaweeds. The char content of the seaweeds did not change in response to any of the manipulated factors, and the only significant effect of changes in pCO2 was a negative relationship with phenolic content. We conclude that the optimal cultivation conditions for Swedish U. fenestrata are dependent on the desired biomass traits (biomass yield or biochemical composition).

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Cultivation conditions affect the monosaccharide composition in Ulva fenestrata

Cited by 19 publications

References 38 publications

Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

Effects of geographical location on potentially valuable components in Ulva intestinalis sampled along the Swedish coast

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Effects of irradiance, temperature, nutrients, and pCO2 on the growth and biochemical composition of cultivated Ulva fenestrata

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