Cultivation media for lactic acid bacteria used in dairy products

Hayek, Saeed A.; Gyawali, Rabin; Aljaloud, Sulaiman O.; Krastanov, Albert; Ibrahim, Salam A.

doi:10.1017/s002202991900075x

Cited by 84 publications

(86 citation statements)

References 106 publications

(214 reference statements)

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“…Nevertheless, this aim remains challenging, particularly for Lactobacillus and Bifidobacterium species. In fact, some selective media can impair growth of the target microbe or allow growth of other microorganisms, thus resulting not completely selective ( Roy, 2001 ; Davis, 2014 ; Hayek et al, 2019 ; Margolles and Ruiz, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

et al. 2021

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On the worldwide market, a great number of probiotic formulations are available to consumers as drugs, dietary supplements, and functional foods. For exerting their beneficial effects on host health, these preparations should contain a sufficient amount of the indicated living microbes and be pathogen-free to be safe. Therefore, the contained microbial species and their amount until product expiry are required to be accurately reported on the labels. While commercial formulations licensed as drugs are subjected to rigorous quality controls, less stringent regulations are generally applied to preparations categorized as dietary supplements and functional foods. Many reports indicated that the content of several probiotic formulations does not always correspond to the label claims in terms of microbial identification, number of living organisms, and purity, highlighting the requirement for more stringent quality controls by manufacturers. The main focus of this review is to provide an in-depth overview of the microbiological quality of probiotic formulations commercialized worldwide. Many incongruences in the compositional quality of some probiotic formulations available on the worldwide market were highlighted. Even if manufacturers carry at least some of the responsibility for these inconsistencies, studies that analyze probiotic products should be conducted following recommended and up-to-date methodologies.

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Section: Discussionmentioning

confidence: 99%

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

et al. 2021

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“…2016), and considering that LAB are nutritionally demanding, whey permeate should be supplemented with nitrogen sources (Hayek et al . 2019). In this work, peptone and yeast extract were added to whey permeate‐based culture media.…”

Section: Discussionmentioning

confidence: 99%

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

et al. 2020

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Aims To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4°C after spray‐drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150°C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3·96, 73·68 and 90·36% respectively. Those conditions also showed a decrease of 0·855 log CFU per gram after drying, no loss in viability at 4°C for 6 months and 1 log CFU per gram reduction at 25°C. Conclusions Stable and economically feasible dehydrated LAB cultures were obtained using alternative culture media, fermentation under uncontrolled pH and optimizing spray‐drying process conditions through the desirability function method. Significance and Impact of the Study Our results can be utilized for efficient production and commercialization of several dry LAB.

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“…Glucose, lactose, aerosil, starch and intestinal soluble capsules were used in various ratios as constituents of the probiotic. Cultivation of lactobacilli and bifidobacteria was carried out on MRS agar and Blaurock nutrient medium as well as on a skim milk at 37 °C for 24-72 hours (Süle et al, 2014;Hayek et al, 2019;Paliy et al, 2020c). A lyophilic drying of lactic acid bacteria was carried out on the "LZ-4527" installation according to the following technological regime: the temperature increased from minus 72 °C to 26 °C.…”

Section: Methodsmentioning

confidence: 99%

Development of technical regulations for the capsulated probiotic manufacture

Paliy¹,

Gujvinska²,

Kalashnyk³

2020

Ukr J Ecol

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The aim of this work was to develop a technological regulation for the production of probiotic based on lacto- and bifidobacteria in the form of intestinal-soluble capsules for farm animals. Three experimental series of probiotic drug were made in the form of enteric capsules based on lactic acid bacteria. It has been established that the Bifidobacterium adolectentis No 17-316 strain and the Lactobacillus plantarum No 7-317 strain are the most adapted for the bacterial consortium. These strains have high biological activity and correspond to certain authentic characteristics. The pharmaceutical composition is proposed in the form of capsules with following ingredients: 50% of dry biomass of Lactobacillus plantarum No 7-317 and Bifidobacterium adolectentis No 17-316 strains, 5% of glucose, 5% of lactose, 9% of aerosil and 31% of starch. The content of lyophilized bacteria in one capsule is not less than 1 × 107 CFU of bifidobacteria/cm3 and 1 × 107 CFU of lactobacilli/cm3. A scheme of the manufacturing process of the drug in form of capsules includes the following stages: production of nutrient media and working solutions; growing cultures of lacto- and bifidobacteria, freeze drying of cultures of lacto- and bifidobacteria, obtaining a dry mass of lactic acid bacteria for encapsulation, encapsulation of the drug, control of the manufactured product before release, marking, packaging of capsules, transportation, storage of a drug.

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Cultivation media for lactic acid bacteria used in dairy products

Cited by 84 publications

References 106 publications

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

Development of technical regulations for the capsulated probiotic manufacture

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