Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol

Torre, José R. de la; Walker, Christopher B.; Ingalls, Anitra E.; Könneke, Martin; Stahl, David A.

doi:10.1111/j.1462-2920.2007.01506.x

Cited by 604 publications

(546 citation statements)

References 44 publications

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“…The information about the effect of heavy metal on AOA was still limited up to now. Although it was reported that AOA might be in general less susceptible than AOB to inhibitor because of the remarkably different physiological characteristics of archaea compared with bacteria (Schauss et al 2009), the mechanistic information about the response of AOA to environmental stress remains to be unclear due to limited cultivated AOA cluster so far (Konneke et al 2005;de la Torre et al 2008). According to the results in this study, it was not an appropriate option to use the amoA gene copy number of AOB or AOA for the purpose of soil pollution assessment.…”

Section: Discussionmentioning

confidence: 74%

Effects of mercury on the activity and community composition of soil ammonia oxidizers

Liu

Zheng

Shen

et al. 2010

Environ Sci Pollut Res

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Purpose Experiments were conducted to examine the effects of mercury (Hg) on soil nitrification activities and the microbial communities of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). Methods The soil samples spiked with different Hg concentrations were incubated for a period of 1, 2, 4, and 8 weeks in triplicate and the potential nitrification rate (PNR) of the samples was determined. The abundance of AOB and AOA was measured after an 8-week incubation by real-time polymerase chain reaction (PCR) assay of the amoA genes, while the community compositions by cloning and sequencing approaches. Results The soil PNR differed with different incubation periods. It tended to decrease with increasing soil Hg concentrations at week1, basing on which the half-maximal effective concentration (EC50) was 1.59 mg kg −1 . There was no significant difference in the abundance of AOB or AOA among the treatments. The AOB community was dominated by Nitrosospira-like sequences and more than 70% of the obtained clones were affiliated with the cluster 3a.2. The percentage of cluster 3a

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Section: Discussionmentioning

confidence: 74%

Effects of mercury on the activity and community composition of soil ammonia oxidizers

Liu

Zheng

Shen

et al. 2010

Environ Sci Pollut Res

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“…The recent discovery of 'Candidatus Nitrosocaldus yellowstonii', an archaeon that grows up to 74°C, however broadened the phylogenetic spectrum of ammonia oxidizers active at high temperatures (de la Torre et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

et al. 2016

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The increasing production of nitrogen-containing fertilizers is crucial to meet the global food demand, yet high losses of reactive nitrogen associated with the food production/consumption chain progressively deteriorate the natural environment. Currently, mesophilic nitrogen-removing microbes eliminate nitrogen from wastewaters. Although thermophilic nitrifiers have been separately enriched from natural environments, no bioreactors are described that couple these processes for the treatment of nitrogen in hot wastewaters. Samples from composting facilities were used as inoculum for the batch-wise enrichment of thermophilic nitrifiers (350 days). Subsequently, the enrichments were transferred to a bioreactor to obtain a stable, high-rate nitrifying process (560 days). The community contained up to 17% ammonia-oxidizing archaea (AOAs) closely related to 'Candidatus Nitrososphaera gargensis', and 25% nitrite-oxidizing bacteria (NOBs) related to Nitrospira calida. Incorporation of 13 C-derived bicarbonate into the respective characteristic membrane lipids during nitrification supported their activity as autotrophs. Specific activities up to 198 ± 10 and 894 ± 81 mg N g − 1 VSS per day for AOAs and NOBs were measured, where NOBs were 33% more sensitive to free ammonia. The NOBs were extremely sensitive to free nitrous acid, whereas the AOAs could only be inhibited by high nitrite concentrations, independent of the free nitrous acid concentration. The observed difference in product/substrate inhibition could facilitate the development of NOB inhibition strategies to achieve more cost-effective processes such as deammonification. This study describes the enrichment of autotrophic thermophilic nitrifiers from a nutrient-rich environment and the successful operation of a thermophilic nitrifying bioreactor for the first time, facilitating opportunities for thermophilic nitrogen removal biotechnology.

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“…in situ production of a small amount of IPL iGDGTs in the oxidized sediment by sedimentary aerobic Thaumarchaeota. These likely function via aerobic oxidation of ammonium (Könneke et al, 2005;Wuchter et al, 2006b;de la Torre et al, 2008;Walker et al, 2010;Tourna et al, 2011), formed from the break-down of OM when O 2 was penetrating into the sediments. To examine the oxic degradation and potential production of individual IPL iGDGTs, we performed direct analysis of IPL crenarchaeol.…”

Section: Oxic Degradation Of Igdgts CL and Iplmentioning

confidence: 99%

Differential degradation of intact polar and core glycerol dialkyl glycerol tetraether lipids upon post-depositional oxidation

Lengger

Kraaij

Tjallingii

et al. 2013

Organic Geochemistry

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Archaeal and bacterial glycerol dialkyl glycerol tetraether lipids (GDGTs) are used in various proxies, such as TEX 86 and the BIT index. In living cells, GDGTs contain polar head groups (intact polar lipids -IPLs). IPL GDGTs have also been detected in ancient marine sediments and it is unclear whether or not they are fossil entities or are part of living cells. In order to determine the extent of degradation of IPL GDGTs over geological timescales, we analyzed turbidite deposits, which had been partly reoxidized for several kyr after deposition on the Madeira Abyssal Plain. Analysis of core lipid (CL) and IPL-derived GDGTs showed a reduction in concentration by two orders of magnitude upon post-depositional oxidation, while IPL GDGTs with a mono-or dihexose head group decreased by 2-3 orders of magnitude. The BIT index for CL-and IPL-derived GDGTs increased substantially upon oxidation from 0.1 to up to 0.5. Together with changing MBT/ CBT values, this indicates preferential preservation of soil-derived branched GDGTs over marine isoprenoid GDGTs, combined with in situ production of branched GDGTs in the sediment. The TEX 86 value for IPL-derived GDGTs decreased by 0.07 upon oxidation, while that of CL GDGTs showed no significant change. Isolation of IPLs revealed that the TEX 86 value of monohexose GDGTs was 0.55, while the TEX 86 value for dihexose GDGTs was substantially higher, 0.70. Thus, the decrease in TEX 86 for IPL-derived GDGTs was in agreement with the dominance of monohexose GDGTs in the oxidized turbidite, probably caused by a combination of in situ production as well as selective preservation of terrestrial isoprenoid GDGTs. Due to the low amount of IPL GDGTs vs. CL GDGTs, the impact of IPL degradation on CL-based TEX 86 paleotemperature estimates is negligible.

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Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol

Cited by 604 publications

References 44 publications

Effects of mercury on the activity and community composition of soil ammonia oxidizers

Effects of mercury on the activity and community composition of soil ammonia oxidizers

A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

Differential degradation of intact polar and core glycerol dialkyl glycerol tetraether lipids upon post-depositional oxidation

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