Cultivation of Edible Tropical Bolete, Phlebopus spongiosus, in Thailand and Yield Improvement by High-Voltage Pulsed Stimulation

Abstract

Tropical bolete, Phlebopus spongiosus, is an edible ectomycorrhizal mushroom indigenous to northern Thailand. This mushroom has the ability to produce fruiting bodies without the need for a host plant. In this study, the technological cultivation of P. spongiosus was developed. Cultivation experiments indicated that fungal mycelia could completely colonize the cultivation substrate over a period of 85–90 days following inoculation of liquid inoculum. Primordia were induced under lower temperatures, high humidity and a 12-h photoperiod. Mature fruiting bodies were developed from young fruiting bodies within a period of one week. Consequently, yield improvement of P. spongiosus cultivation was determined by high-voltage pulsed stimulation. The results indicated that the highest degree of primordial formation, number of mature fruiting bodies and total weight values were obtained in cultivation experiments involving a high voltage of 40 kV. The total weight of the mushrooms increased by 1.4 times after applying high-voltage pulses when compared with the control. Additionally, the results revealed that the size of the fruiting body and the proximate composition of the fruiting bodies from high-voltage stimulation treatments were not different from the control. This research provides valuable information concerning successful cultivation techniques and yield improvement by high-voltage pulsed stimulation for the large-scale commercial fruiting body production of P. spongiosus.

Biochemical, nutritional, and toxicological properties of the edible species Phlebopus beniensis with ethnomycological notes from Paraguay

“…Our result for P. beniensis, obtained with the anthrone method (specific for carbohydrates) was 13%, much lower than other edible Phlebopus species (45% to 55%). However, those percentages were calculated with the difference method (Liaotrakoon & Liaotrakoon, 2018;Kumla et al, 2021Kumla et al, , 2022 which could be inaccurate for the correct determination of proximate composition.…”