Cultivation of Excised Stem Tips of Asparagus in Vitro

Loo, Shih-Wei

doi:10.1002/j.1537-2197.1945.tb05081.x

Cited by 48 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result contradicts the interpretation of de Ropp "(1945) that the stem tip is not physiologically adapted to absorb already elaborated nutrients direct from an external medium. The earlier works of La Rue (1936) and Loo (1945) also agree with the present writer's results.…”

supporting

confidence: 93%

“…The problems of differentiation, or ma-the effects of various accessory growth factors in turation of tissues down the plant shoot, have been the medium on the rates of growth. Loo (1945) subjected to few physiological studies. Higher grew stem tips (5 to 10 mm, long) of Asparagus plants, with the young, embryonic tissues at the officinalis in sterile culture through twenty successtem tip, and progressively more differentiated or sive transfers.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Development in Sterile Culture of Stem Tips and Subjacent Regions of Tropaeolum Majus L. And of Lupinus Albus L

Ball

1946

American J of Botany

View full text Add to dashboard Cite

THEORETICALLY, the shoot apex of higher plants that length rooted only in light. Large plants were should prove the ideal object for experimental mor-grown from these cultures. Smith (1940) grew stem phology. It produces by its activities tissues from tips (0.55 to 4.0 mm, long) of Helianthus annuus which all the vegetative parts of the shoot are dif-on agar in White's solution. Studies were made of ferentiated. The problems of differentiation, or ma-the effects of various accessory growth factors in turation of tissues down the plant shoot, have been the medium on the rates of growth. Loo (1945) subjected to few physiological studies. Higher grew stem tips (5 to 10 mm, long) of Asparagus plants, with the young, embryonic tissues at the officinalis in sterile culture through twenty successtem tip, and progressively more differentiated or sive transfers. He considered these cultures to be mature tissues in the subjacent regions, are excel-the first true stem (organ) cultures that have been lent material for in vitro studies.achieved and to be comparable with the organ cul-Little consideration in the published work has tures of excised roots described by White, Robbins, been given to such significant morphological char-Bonner and others. De Ropp (1945) reported that acteristics as polarity, potentiality for development the growth of excised stem tips of rye embryos was of tissues during maturation, and correlation. The confined to the first leaf. When roots were produced, great theoretical interest of such data prompted the which was rarely, growth was more normal. writer to carry out several series of sterile cultures Much controversy has occurred in the literature of shoot apices (with little additional tissues), and concerning the capacity for growth and developof subjacent tissues undergoing maturation. ment of vegetative plant cells. It was the purpose Rechinger (1893) grew isolated buds of Populus of the writer to obtain evidence on this matter by nigra, Fraeinus ornus and other plants in sand with-comparing the growth in vitro of the apical, meriout nutrient materials. Some basal callus and a stematic tissues with that of the subjacent maturslight expansion of upper parts occurred, but no ing tissues of the shoot. roots were formed. Goebel (1908) grew isolated Also, since the respiratory rates of the tissue buds of Ceratopteris thalictroides in moist peat and pieces used in these experiments had been previfound that they produced leaves much smaller and ously determined (Ball and Boell, 1944), it was simpler morphologically than those of normal considered worth while to find whether a relationplants. Robbins (1922) apparently first grew shoot ship exists between rate of respiration and developtips in sterile conditions with sugar. He grew stem mental capacity. tips (1.75 to 3.75 em, long) of pea, corn and cotton Further, these cultures lent themselves well to in the dark in agar with glucose. The tips produced studies of the polarity of the buds and subjacent shoots with small chlorotic leaves and numerous...

show abstract

supporting

confidence: 93%

mentioning

confidence: 99%

Development in Sterile Culture of Stem Tips and Subjacent Regions of Tropaeolum Majus L. And of Lupinus Albus L

Ball

1946

American J of Botany

View full text Add to dashboard Cite

show abstract

“…Robbins (1922) seems to have been the first person to have successfully cultured excised shoot tips on a medium containing sugar, but a significant shoot growth from vegetative shoot tip explants was first achieved by Loo (1945aLoo ( , b, 1946a in Asparagus and dodder plants. He has made several significant observations in Asparagus cultures showing that:…”

Section: The Propagation Of Plants From Axillary Buds or Shootsmentioning

confidence: 98%

“…Lyophilized leaf extract from leaves of dodder host plants (a preparation which probably contained auxin) and pure IAA were incorporated in culture media with mixed results starting about a decade after the discovery of this hormone (Fiedler 1936;Geiger-Huber and Burlet 1936;Gautheret 1935Gautheret , 1937Loo 1945a). Skoog and Tsui (1951) reported the continued cell division and bud formation in the cultured pith tissues of tobacco on nutrient media containing adenine and high levels of phosphate.…”

Section: Introductionmentioning

confidence: 98%

Review of Literature

Varshney¹,

Anis²

2014

Trees: Propagation and Conservation

View full text Add to dashboard Cite

Gottlieb Haberlandt (Math Naturwiss 111:69-92), a German plant physiologist, for the first time initiated the work on tissue culture. His work arose as a research tool and attempts were made to culture the isolated, fully differentiated cells in nutrient medium in vitro as early as 1898. The theoretical basis of tissue culture lies in the cell theory given by Schleiden and Schwann (1838-1839). Practically, this technique stands on the concept of "totipotency," i.e., each cell has the ability to regenerate into a new plant. The field finds a wide range of applications starting from mass clonal propagation to plant improvement, molecular biology, bio-processing as well as a basic research tool. It has advanced the production in forestry and agriculture to many folds. There are a number of reviews published on tissue culture of woody and tree species which provide the wide-ranging micropropagation reports of various plant species. In this way, this chapter highlights the current review in tree tissue culture in vitro and micropropagation of other valuable plants, their significance, and the wide scope existing for investigations on mass multiplication and conservation of these plants.

show abstract

“…In the following few years, single somatic cells of some green plants have been induced to develop into entire individuals and eventually produce flowers and fruits (Vasil & Hilderbrandt, 1965). In addition, studies of plant tissue culture in monocotyledons were a bit later than that in dicotyledons: Loo (1945) firstly performed stem cultures in vitro from apical meristems of monocotyledonous Asparagus officinalis; until in 1951, Morel & Wetmore (1951) successfully obtained the proliferation in vitro from tuber of monocotydelonous Amorphophallus rivieri. Based on one hundred years' investigation, plant tissue culture technologies have achieved a great progress in many aspects including the effects of plant growth regulators, auxins, and cytokinins, genotype-dependence, callus type-dependence and so on.…”

Section: Introductionmentioning

confidence: 99%