2016
DOI: 10.1002/cjce.22407
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Cultivation of freshwater green alga Neochloris oleoabundans in non‐sterile media co‐inoculated with protozoa

Abstract: The effects of sodium bicarbonate on freshwater green alga Neochloris oleoabundans were evaluated with respect to cell growth, cell morphology, and control of protozoa contamination under non-sterile conditions co-inoculated with protozoa pre-cultures. While culture collapse occurred in the control and the cultures containing 40 mmol/L NaHCO 3 due to protozoa contamination, the addition of 160 or 200 mmol/L NaHCO 3 could completely inhibit the growth of microalgae-preying protozoa and allow safe cultivation of… Show more

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Cited by 12 publications
(12 citation statements)
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“…Given that protozoa are do not have protective cell wall, they might be much more sensitive to osmotic pressure than microalgae and other microorganisms with cell wall (Peng et al, 2015), which is demonstrated clearly with the results of this study.…”
Section: Figsupporting
confidence: 69%
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“…Given that protozoa are do not have protective cell wall, they might be much more sensitive to osmotic pressure than microalgae and other microorganisms with cell wall (Peng et al, 2015), which is demonstrated clearly with the results of this study.…”
Section: Figsupporting
confidence: 69%
“…A recent study has shown that safe cultivation of N. oleoabundans in non-sterile media with nonsterile aeration is achievable by adding appropriate amount of NaHCO 3 in the media (Peng et al, 2015). However, pH was not controlled in these experiments of the recent study, which increased from 7.5 in the beginning to approximately 10.5 at the end of cultivation.…”
Section: Introductionmentioning
confidence: 93%
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“…Previous studies proposed that one possible mechanism for selective inhibition of protozoa in N. oleoabundans cultivation is osmotic pressure . It was reasoned that microalgae can adapt to hyper‐osmotic environment by accumulation of compatible osmolytes and fresh green algae N. oleoabundans can resist osmotic pressure imposed by NaCl up to 600 mM by converting polysaccharides to sucrose . Besides, the rigid cell wall in microalgae could protect cells from osmotic stress, whereas the wall‐less protozoa cells may be more susceptible to high osmotic pressure .…”
Section: Resultsmentioning
confidence: 99%
“…Our previous studies demonstrated that adding 160 mM NaHCO 3 to the modified Bristol medium (MBM) at pH 9.5 can prevent protozoa contamination, enables safe cultivation of fresh water microalgae Neochloris oleoabundans and induces high lipids production at long term under unsterile conditions . However, the mechanism by which the sodium bicarbonate contributed to protozoa cell death is unclear.…”
Section: Introductionmentioning
confidence: 99%