Cultivation of hamster bone marrow haematopoietic stem and progenitor cells

Abstract

Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia

Solar light induced rhodamine B degradation assisted by TiO2–Zn–Al LDH based photocatalysts

“…It was observed that these bands are present but have low intensities indicating small amount of the ZnO phase in this sample. This conclusion is in good agreement with the results from XRD analysis of the ZnAl-LDH sample where the presence of a crystalline ZnO phase dispersed over the brucite-like layers was detected [25]. ZnO phase presence is of great importance due to the fact that this phase has been reported to highly contribute to the photocatalytic activity in the sunlight region [17,46].…”

“…This FTIR spectrum reveals also absorption peaks at 696 and 467 cm À1 that can be attributed to the stretching mode of Al-O in an octahedral coordination state suggesting the transformation of the crystal phase to spinel Al 2 O 4 [42]. These assumptions are verified by previous XRD analysis of this calcined sample where the collapse of the layered structure after calcination was confirmed leading to the zincite, ZnO phase and the spinel phase (ZnAl 2 O 4 ) [25].…”

“…All synthesized samples were investigated by XRD analysis and the detected phases from the XRD spectra reported in our previous work [20,25,31] are presented in Fig. 2.…”

“…2. The LDH phase was detected as the dominant phase in ZnAl-LDH and IM_ZnAl_1Ti samples, whereas in samples C-ZnAl, IM_ZnAl_2Ti and IM_ZnAl_3Ti the detected dominant phase was the ZnO assigned to non-stoichiometric mixed oxides, Zn(Al)O phase, since the origin of the ZnO phase is the Zn-Al LDH (JCPDS 80-0075) [25,35]. After impregnation, although the concentration of TiO 2 was very low, the reflections of the TiO 2 anatase phase (JCPDS 84-1286) was detected in all samples.…”

“…This especially refers to the activity in the sunlight spectrum which is assigned to the ZnO phase developed in Zn(Al)O mixed oxides structure after thermal activation of Zn-Al LDHs [24,25]. The TiO 2 loading amount was varied during the impregnation process onto the calcined LDHs in order to establish the influence of the loading amount on the photocatalytic performances of LDHs.…”