Cultivation of human keratinocytes without feeder cells on polymer carriers containing ethoxyethyl methacrylate: in vitro study

Abstract

The cell/tissue engineering therapy of extensive or chronic skin wounds is a highly topical task of the contemporary medicine. One of possible therapeutic approaches is grafting of in vitro cultured keratinocytes directly to the wound bed, where the cells colonize the wound, proliferate and improve the re-epithelization process. Because the successful cultivation of keratinocytes needs an application of feeder cells, the exclusion of these cells from the cultivation system is highly required. In this study we show a positive influence of 2-ethoxyethyl methacrylate as a component of cultivation support on growth of keratinocytes without feeder cells. Keratinocytes cultured on these surfaces are able to migrate to the model wound bed in vitro, where they form distinct colonies and have a normal differentiation potential.

Electrospinning of the hydrophilic poly (2-hydroxyethyl methacrylate) and its copolymers with 2-ethoxyethyl methacrylate

“…Due to its good solubility in aqueous ethanol and favourable elasticity, we have chosen 2-ethoxyethyl methacrylate (EOEMA). Its copolymer with HEMA is also biocompatible [13,17] and has very promising biological properties [27]. Figure 7 and 8, η sp /c vs. c dependences for HEMA -EOEMA copolymers and the dependence of intrinsic viscosity on the HEMA content are shown (the Kraemers method gave almost the same values).…”

“…This indicates the possible presence of large structures or even microgels in the original solution, which hinder the formation of Taylor cones. Two samples showing opposite behaviour were examined by dynamic light scattering (Figure [13]) to examine these larger structures, i. e. poly(HEMA), M w = 6.31 × 10 5 g/mol (spinnable without problems) and HEMA/EOEMA copolymer (30% HEMA) (spinnable after filtration). The poly(HEMA) contained only polymer coils, ca.…”

“…with antibacte-rial properties or with bound antibodies for selective filtration, or air filtration), wound dressing (especially for skin defects and burns), and tissue engineering (in vitro cultivation of cells) may be obtained [12][13][14][15][16][17]. In particular, macroporous materials for central nervous system implants might be prepared with incomparably larger surface area than those prepared by classic methods.…”

Epidermal cells delivered for cutaneous wound healing

“…The technique has been fully reviewed in the literature (69). The underlayer of the dermal equivalent may be substituted by a dermal scaffold such as acellular biological matrices or synthetic bio-degradable polymers in a variety of forms (59,61,66,(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80)(81)(82)(83)(84)(85)(86) or added to other ingredients, such as growth factors or proteinases, into the collagen gel to enhance the quality of the sheet or expedite wound healing (87)(88)(89)(90)(91)(92)(93)(94).…”

Re-engineering islet cell transplantation

“…In general, natural materials have the benefit of high cellular recognition and tissue compatibility, but are costly, variable, biodegradable, and require high levels of purification [98]. Synthetic materials, on the other hand, can be fabricated with uniformity between batches, precise control over rates of degradation, and modified chemical properties.…”