Cultivation of L Strain (Earle) Mouse Cells in Bacteriological Media Combined with Horse Serum

Detection, Elimination, and Prevention of Contamination of Cell Cultures With Pleuropneumonialike Organisms

“…3 (Difco) medium containing 10 or 20 per cent horse serum (YE-PP no. 390HoSj0) (Mayyasi and Schuurmans, 1956), (b) a 0.5 per cent lactalbumin hydrolyzate medium in Hanks' balanced salt solution with 10 per cent calf serum added (lacto.5BSS89.5CaSjo), (c) Eagle's medium (1955) containing 10 per cent horse serum (EaglegoHoS,o), (d) medium 199 (Morgan et al, 1950) containing 20 per cent horse serum (1998oHoS20). The horse serum was obtained from horses quartered at Fort Detrick.…”

The Growth of Asibi Strain Yellow Fever Virus in Tissue Cultures. I. Sensitivity and Capacity of Tissue Cultures

“…It Was foin4 in further experiments that no effect due to'wanthing.was observed beyond two hours post-inoculation 6 In view of the results shown in Figures I and 2, an experimunt wast n.xt 'dosigned to test the effect of washing on the sensittvlty and capacity of the HeLa cells., ,Five HeLa cell flask cultures were subjected to a.preinoculti6n wash cycle". Each culture was inoculated with a one-milliliter dl.tftion of Asibi strain virus.…”

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