Cultivation of Lovage under Exposure of Light-Emitting Diode Illumination and Analysis of Rutin Produced by High Performance Liquid Chromatography (HPLC) and Ultraviolet-Visible Spectroscopy (UV-Vis)

Abstract

To meet the requirements of a sustainable agricultural economy such as quality, resource conservation, and efficiency, the interaction between the cultivation parameters of plants and the resulting plant ingredients are becoming more and more interesting, especially regarding secondary metabolites. Therefore, the cultivation of lovage under the influence of light-emitting diode illumination and controlled environment conditions was studied and data on the rutin concentrations obtained are presented. Different extraction agents and processes were tested for the treatment of the leaves and the rutin was analyzed using high performance liquid chromatography (HPLC) and ultraviolet-visible spectroscopy (UV-Vis spectroscopy). UV-Vis spectroscopy was found to provide a simple and rapid method of predicting the rutin concentration. The significant parameter regarding the rutin concentration obtained was light intensity and overall rutin concentrations in the range of 17,005 mg to 34,759 mg of rutin per kg dry leaves were found.

How does supplementary green light and UV-radiation affect biomass and rutin content in Levisticum officinale?

“…With respect to our study from 2022, where no clear significance could be shown between the effect of two green light proportions (20 and 40% of the total PPFD) on rutin content, the presented work investigated the potential effect of lower green light proportions (Wack et al, 2022). The results indicate that green light as a sole factor does not have a significant effect, even at low levels.…”

“…In our review from 2020, we point out partially contrary results of the effect of various light treatments on flavonoid content, even for plants belonging to the same family or variety (Thoma et al, 2020). Based on our results from 2022 regarding rutin content in lovage (Levisticum officinale W.D.J KOCH), no clear significant effect of specific wavelength components could be shown (Wack et al, 2022). No significant difference between two Thoma et al 10.3389/fsufs.2023.1322443 Frontiers in Sustainable Food Systems 02 frontiersin.org relatively high proportions of green light (20 and 40% of the total photosynthetic photon flux density (PPFD)) where shown.…”

“…Most studies about lovage investigate its essential oils, their composition or germination response (Raal et al, 2008;Khodashenas et al, 2015;Spréa et al, 2020). Wack et al found one of the significant parameters regarding rutin concentration in lovage was light intensity and developed a new extraction method for rutin (Wack et al, 2022). Furthermore, Lima et al aimed in their study to evaluate the influence of red, blue and white and as well monochromatic light associated or not with UV-B on photosynthetic performance and phenolic compound production in microtomato plants cultivated via vertical agriculture.…”