Cultivation of Non-Proliferating Rat Heart Cells in a Roller System at Oxygen Tensions down to 0.66 mmHg

Wollenberger, A; Karsten, Uwe; Kossler, Andrea L

doi:10.1159/000169366

Cited by 7 publications

(1 citation statement)

References 5 publications

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“…However, biochemical studies on such systems as those of Ziter (1974) are complicated by the presence of non-muscle cells, which have been described as fibroblast-like cells (Polinger, 1970(Polinger, , 1973Chacko & Joseph, 1974) and endothelial cells (Kasten & Yip, 1974). Although cardiac muscle cells appear to be non-dividing in some systems (Halle & Wollenberger, 1970;Wollenberger et al, 1972), definite evidence for division at a lower rate than in non-muscle cells has been obtained by others (Chacko, 1973;Polinger, 1973). Cardiac muscle cells are therefore fairly quickly overgrown in cultures by non-muscle cells (Polinger, 1973), although such overgrowth can be prevented by treatment of cultures with the mitotic inhibitor 5-fluorodeoxyuridine (Karsten et al, 1973;Wollenberger et al, 1972) and by glutamine deprivation (Clark, 1976).…”

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Effects of 5-bromo-2'-deoxyuridine on beating heart cell cultures from neonatal hamsters

Coetzee¹,

Westhuyzen²,

Gevers³

1977

Biochemical Journal

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1. Primary heart cell cultures from neonatal hamsters yielded a heterogeneous cell population, containing muscle cells undergoing progressive differentiation, as well as non-muscle cells. 2. Addition of 5-bromo-2'-deoxyuridine, at an early stage, to such cultures enhanced the formation of beating sheets of differentiated muscle cells. Accumulation of myosin heavy chains and creatine kinase also occurred in the presence of the analogue. 3. To obtain these effects, the analogue had to be added during the initial rapid growth phase of the cells. Division of the treated cells then ceased when the cell numbers had approximately doubled. 4. Similar results were obtained with other inhibitors of DNA synthesis. Thus improved muscle cell cultures can be obtained by preventing non-muscle cells from overgrowing the cultures. 5. One effect caused only by 5-bromo-2'-deoxyuridine was a large increase in the Ca2+-stimulated ATPase (adenosine triphosphatase) activity which sedimented at low ionic strength. This increase was not due to a greater content of myofibrillar myosin, or to myosin isoenzyme changes, because purified myosin prepared from treated and untreated cultures did not exhibit the increased Ca2+-stimulated ATPase activity.

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confidence: 99%

Effects of 5-bromo-2'-deoxyuridine on beating heart cell cultures from neonatal hamsters

Coetzee¹,

Westhuyzen²,

Gevers³

1977

Biochemical Journal

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Reaction of Cultured Carotid Body Cells to Different Concentrations of Oxygen and Carbon Dioxide

Pietruschka¹,

Schäfer²,

Lübbers³

1977

Chemoreception in the Carotid Body

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Ischemic myocardial injury in cultured heart cells: Preliminary observations on morphology and beating activity

Acosta

Puckett

1977

In Vitro

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An in vitro model of myocardial ischemia has been established with primary monolayer cultures of neonatal rat heart cells. Ischemic conditions were simulated in vitro by subjecting the heart cell cultures to various levels of oxygen and glucose deprivation. After the ischemic treatments, cultures of beating muscle (M) cells were evaluated for functional and morphological changes. The experimental protocol consisted of treatment with 20% or 0% O2 and 1000, 500 or 0 mg glucose per 1 of medium for 4, 12 or 24 hr. Control cultures were treated with 20% O2 and 1000 mg glucose. The morphological alterations induced by the deficiency of O2 and glucose in the medium were the formation of pseudopodia and cytoplasmic vacuoles; increased cytoplasmic granulation; and the formation of abnormal cell shapes, such as long, spindly shaped M cells. There was a time-dependent decrease in beating activity as the M cells were exposed to longer durations of ischemic conditions. However, if the cultures were replenished with complete medium (1000 mg glucose) and 20% O2, the cells regained their ability to beat.

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Cultivation of Non-Proliferating Rat Heart Cells in a Roller System at Oxygen Tensions down to 0.66 mmHg

Cited by 7 publications

References 5 publications

Effects of 5-bromo-2'-deoxyuridine on beating heart cell cultures from neonatal hamsters

Effects of 5-bromo-2'-deoxyuridine on beating heart cell cultures from neonatal hamsters

Reaction of Cultured Carotid Body Cells to Different Concentrations of Oxygen and Carbon Dioxide

Ischemic myocardial injury in cultured heart cells: Preliminary observations on morphology and beating activity

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