Ocean acidification (OA) and coastal eutrophication affect coastal marine organisms. We studied the physiological responses of Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta) to increased concentrations of CO 2 and NH 4 + . Incubation treatments were applied at two different pH units (low, 7.5; high (control), 7.9) and three different NH 4 + concentrations (low, 10; medium, 50; high, 100 μM). Growth, rates of photosynthetic oxygen evolution, and NH 4 + uptake rates were affected by both elevated CO 2 and NH 4 + conditions. The changes in the pH of culture media were influenced by elevated CO 2 or NH 4 + treatments. However, chlorophyll fluorescence was affected only by the level of NH 4 + . These results indicate that the physiological responses of G. lemaneiformis might be enhanced when the concentrations of CO 2 and NH 4 + rise. Therefore, cultures of this alga could provide a good mitigation solution against ongoing problems with OA and coastal eutrophication.