Cultivation of the yeastCandida lipolytica on hydrocarbon

Munk, V.; Volfová, O.; Dostálek, Milan; Mostecký, J.; Pecka, K.

doi:10.1007/bf02872701

Cited by 16 publications

(8 citation statements)

References 16 publications

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“…XI, ISSUE 3 of real systems, it should be possible to evaluate the parameters of the models using batch fermentation data, and then closely predict the behavior of continuous fermentation systems based on the results of the batch fermentation data. In Figure 20, we have attempted to do this for the data of Alunk et al 3 Using the batch fermentation parameter values reported in the previous paper' of pmnx = 0.335 hr-', Nd/YfdSO = 0.25, Ks/So = 0.01 and assuming the yield to be the same for batch and continuous fermentation, one can predict the behavior of a continuous fermentor a t any dilution rate. The continuous curve in Figure 20, which is obtained by using equations (7), (ll), and (14) and the above parameter values, is for Case 1 which assumes all growth to be a t the surface of the hydrocarbon drops.…”

Section: Discussion Of Resultsmentioning

confidence: 98%

“…In practice, there may be adsorption and desorption of cells from the surface of the hydrocarbon drops such that the drop surfaces are never completely covered BIOTECHNOLOGY AND BIOENGINEERING, VOL. XI, ISSUE 3 with cells. There may also be more than one layer of cells attached to the drop.…”

Section: Discussion Of Resultsmentioning

confidence: 99%

“…VOL. XI, ISSUE 3 this region of operation. Likewise for dimensionless dilution rates in the region 0.8 to 1.0, the results for Case 2 and Case 4 are similar indicating that continuous phase growth is occuring about as rapidly as the phase equilibrium constant K , and the saturation constant K 1 will allow.…”

Section: Case 4 Continuous Phase Growth Limited By Both Transport Anmentioning

confidence: 95%

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Growth models of cultures with two liquid phases. III. Continuous cultures

Erickson

Humphrey

1969

Biotech & Bioengineering

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SummarySome mathematical models, which have been used to describe batch growth in fermentations with two liquid phases present, are used to predict the behavior of continuous fermentations in a chemostat. Two types of dispersed systems are considered in this investigation. In the first type, it is assumed that the composition of the dispersed phase is such that increased substrate utilization results in a decreased substrate concentration with no change in the interfacial area. In the second type of system, the dispersed phase is assumed to be pure substrate; therefore, the substrate concentration in the dispersed phase remains constant but the interfacial area is affected by changes in dilution rate. Three special cases are examined for each type of system in order to examine the effect of the interfacial area, the phase equilibrium constant, and the mass transfer coefficient on system performance. Comparison of two of the models with available experimental data shows fair agreement between model and data.In two previous growth models for cultures with two liquid phases were considered and equations to predict batch growth were developed. It is well known that kinetic models developed from batch data can often be used together with flow models to predict the performance of continuous systems. The simple kinetic models introduced previously's2 will be used together with a complete mixing flow model to investigate the implicatioiis of the kinetic models for continuous cultivation in a chemostat. Although very little experimental data on continuous cultivation with two liquid phases present has appeared in the l i t e r a t~r e ,~.~ it is important to examine the behavior of continuous systems because of the widespread interest in the fermentation of hydrocarbons. 489

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Section: Discussion Of Resultsmentioning

confidence: 98%

Section: Discussion Of Resultsmentioning

confidence: 99%

Section: Case 4 Continuous Phase Growth Limited By Both Transport Anmentioning

confidence: 95%

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Growth models of cultures with two liquid phases. III. Continuous cultures

Erickson

Humphrey

1969

Biotech & Bioengineering

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“…The factor, necessary for calculation of equivalent dry weight, was estimated from the boundary conditions of fermentation, e.g., from the amount of nitrogen and biomass a t the beginning and end points of fermentation. The value of this factor varied from 9 to 15 g cells dry weightlg nitrogen, according to experimental conditions, and differed from the theoretical value 12.5, estimated by Munk et al, 6 which assumes constant protein content of dry weight during the entire fermentation.…”

Section: Methodsmentioning

confidence: 99%

“…The sediment on the frit was washed with acetone and petroleum ether in order to remove residual hydrocarbons and was then dried a t 105°C to constant weight. 6 Ammoniacal nitrogen was determined in the fermentation medium by filtration after distillation from a Markham apparatus,lo using an indicator according to Ma and Zuaaaga. " Besides the direct dry weight estimation of the population density, the growth kinetics were followed indirectly from the consumption of ammoniacal nitrogen in the fermentation medium during the cultivation.…”

Section: Methodsmentioning

confidence: 99%

Growth models of cultures with two liquid phases. V. Substrate dissolved in dispersed phase—experimental observations

Prokop

Erickson

Paredes‐López

1971

Biotech & Bioengineering

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SummaryThe effects of inoculum size, dispersed phase volume and substrate concentration on the batch growth of Candida lipolytica are investigated in a model system composed of n-hexadecane dissolved in dewaxed gas oil. Tabular values and parameters are presented for 16 different experiments. All of the batch growth curves exhibited a linear growth region with the length of the region ranging from 1.5 to 9.5 hours. The rate of h e a r growth varied both with change in dispersed phase volume and initial dispersed phase substrate concentration. A qualitative analysis of the results is presented and possible explanations for the observed linear growth rates are discussed.

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Russian Article pp. 213–225

1982

Acta Biotechnologica

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The kinetics of assimilation of hydrocarbons having a different structure by Candida yeasts was studied. The rates of oxidation of various carbon atoms in the molecules of isoalkanes, alkylaromatic hydrocarbons and n‐alkanes were evaluated in the range of C11 to C28. Metabolic inhomogeneity of carbon atoms in the molecules of isolakanes and alkylaromatic hydrocarbons was observed. A competition in the assimilation of the called hydrocarbons and n‐alkanes and also a competition in the assimilation of n‐alkanes of a different molecular weight, i.e. metabolic inhomogeneity of carbon atoms of n‐dodecane and n‐tetracosane was found out for yeasts.

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Cultivation of the yeastCandida lipolytica on hydrocarbon

Cited by 16 publications

References 16 publications

Growth models of cultures with two liquid phases. III. Continuous cultures

Growth models of cultures with two liquid phases. III. Continuous cultures

Growth models of cultures with two liquid phases. V. Substrate dissolved in dispersed phase—experimental observations

Russian Article pp. 213–225

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