Cultivation of Toxoplasma in Embryonated Egg. An Antigen Derived from Choriolallantoic Membrane

Warren, Joel; Russ, Sudie B.

doi:10.3181/00379727-67-16213

Cited by 69 publications

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“…Embryonated eggs have been used over decades as a model for protozoan isolation (Buttitta, 1951), propagation (Warren and Russ, 1948 ;Roiron and Galistin, 1963 ;Wunderlin et al 1997), and parasite biology studies (Mello and Deane, 1976 ;Que et al 2004). The use of chicken embryos was a very feasible method to produce T. gondii antigens from the late 1940's until early 1970's, due to its low cost and sterile nature.…”

Section: Sag1 Sag4mentioning

confidence: 99%

“…An additional egg lot with 8 days of incubation was also inoculated with 10 6 tachyzoites, to observe possible age differences in the infection outcome. Inoculation was performed in the chorioallantoic (CA) liquid, according to a previous description (Warren and Russ, 1948). The embryos were observed until hatching ; however, prior mortality was recorded.…”

Section: Animals and Experimental Infectionsmentioning

confidence: 99%

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Neospora caninuminfection in birds: experimental infections in chicken and embryonated eggs

et al. 2007

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S U M M A R YNeospora caninum causes economical impact in cattle-raising farms since it is implicated as the major cause of bovine abortions. Although infection by the parasite has been widely described in mammals, the role of birds in its life-cycle is still obscure. Therefore, this work aimed to evaluate the infection by N. caninum in different chicken models. Experimental infections were conducted in 7-day-old chicks, laying hens and embryonated eggs, where samples were analysed for parasite burden, IgG antibodies and lesions promoted. Chickens demonstrated an asymptomatic infection, although with seroconversion and systemic replication of the parasite. In laying hens, no signs of vertical transmission were observed. However, embryonated eggs inoculated by the allantoic cavity route demonstrated susceptibility to infection, with mortality rates around 50% independent of the inoculum dose. Additionally, dogs became infected after ingestion of different amounts of inoculated eggs, producing either oocysts or specific IgG antibodies. The results herein presented demonstrate that chickens may be intermediate hosts of N. caninum and that embryonated eggs could be a useful model to study the parasite's biology.

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Section: Sag1 Sag4mentioning

confidence: 99%

Section: Animals and Experimental Infectionsmentioning

confidence: 99%

Neospora caninuminfection in birds: experimental infections in chicken and embryonated eggs

et al. 2007

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“…This has led to a search for less exacting but equally effective techniques. Among these have been various types of complement-fixation test (CFT), (Warren and Russ, 1948;Sabin, 1949;Steen and Kass, 1951;Thalhammer, 1956;Fulton and Fulton, 1965), the indirect haemagglutination test (IHAT), (Jacobs and Lund, 1957), the direct agglutination test (DAT), (Fulton and Turk, 1959;Couzineau and Baufine-Ducrocq, 1970), and the immunofluorescent antibody test (IFAT) for detecting either antitoxoplasma IgG/IgM antibodies (Goldman, 1957;Kelen, Ayllon-Leindl, and Labzoffsky, 1962;Fletcher, 1965) or antitoxoplasma IgM antibody alone (Remington, Miller, and Brownlee, 1968;Dropsy, Carquin, and Croix, 1970). In the work reported here, parallel tests have been carried out by these different techniques with sera from large numbers of patients with suspected glandular or ocular toxoplasmosis.…”

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The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis.

Karim¹,

Ludlam²

1975

J Clin Pathol

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Three types of antibody curve have been demonstrated by testing sera during the course of acquired toxoplasmosis by six different techniques. These three types are due to cell-wall antibody, (demonstrated by four of the techniques), to antibody to soluble antigen, and to IgM antibody to the cell wall. These findings have been supported by absorption experiments. A scheme is presented for testing single sera by two or three different tests to indicate the stage and duration of the infection.

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“…The complement fixation test of Warren and Russ (1948) was also carried out on the sera with some of the modifications as adopted by Lainson (1955). The sera for the complement fixation test were inactivated for half an hour in a water-bath at 560 C. They were serially diluted in two-fold dilutions up to a maximum dilution of 1:16, using 0.85% saline.…”

Section: Methodsmentioning

confidence: 99%

The Specificity of the Sabin-Feldman Dye Test With Reference to Protozoal Infections

Kulasiri

1960

J Clin Pathol

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Infections ofEimeria stiedaein normal and splenectomized rabbits and ofLeishmania enriettiiin normal rabbits and guinea-pigs and in splenectomized rabbits did not produce dye test antibodies. Guinea-pigs injected withAtoxoplasma sp.and rabbits immunized with cultures ofCrithidia fasciculatawere found to be negative for dye test antibodies. Mice which had been infected withTrypanosoma cruzidid not show dye test antibodies. All these animals, except one guinea-pig infected withLeishmania enriettii,were negative in the complement-fixation test for toxoplasmosis using the egg antigen. These findings are discussed in the light of previous reports.

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Cultivation of Toxoplasma in Embryonated Egg. An Antigen Derived from Choriolallantoic Membrane

Cited by 69 publications

References 0 publications

Neospora caninuminfection in birds: experimental infections in chicken and embryonated eggs

Neospora caninuminfection in birds: experimental infections in chicken and embryonated eggs

The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis.

The Specificity of the Sabin-Feldman Dye Test With Reference to Protozoal Infections

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